<1. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26778924 AU - Randlett O AU - Wee CL AU - Naumann EA AU - Nnaemeka O AU - Schoppik D AU - Fitzgerald JE AU - Portugues R AU - Lacoste AM AU - Riegler C AU - Engert F AU - Schier AF FA - Randlett, Owen FA - Wee, Caroline L FA - Naumann, Eva A FA - Nnaemeka, Onyeka FA - Schoppik, David FA - Fitzgerald, James E FA - Portugues, Ruben FA - Lacoste, Alix M B FA - Riegler, Clemens FA - Engert, Florian FA - Schier, Alexander F TI - Whole-brain activity mapping onto a zebrafish brain atlas. SO - Nature Methods. 12(11):1039-46, 2015 Nov. AS - Nat Methods. 12(11):1039-46, 2015 Nov. NJ - Nature methods AB - In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal-regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors. IL - 1548-7091 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English <2. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26820010 AU - White SM AU - Huang CJ AU - Huang SC AU - Sun Z AU - Eldredge JD AU - Mallya SM FA - White, Susan M FA - Huang, Chien-Jung FA - Huang, Shao-Ching FA - Sun, Zhengzheng FA - Eldredge, Jeff D FA - Mallya, Sanjay M TI - Evaluation of the Upper Airway Morphology: The Role of Cone Beam Computed Tomography. SO - Journal of the California Dental Association. 43(9):531-9, 2015 Sep. AS - J Calif Dent Assoc. 43(9):531-9, 2015 Sep. NJ - Journal of the California Dental Association AB - Cone beam computed tomography (CBCT) has several applications in dentomaxillofacial diagnosis. Frequently, the imaged volume encompasses the upper airway. This article provides a systematic approach to airway analysis and the implications of the anatomic and pathologic alterations. It discusses the role of CBCT in management of obstructive sleep apnea (OSA) patients. This paper also highlights technological advances that combine CBCT imaging with computational modeling of the airway and the potential clinical applications of such technologies. IL - 1043-2256 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <3. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26457047 AU - Northridge ME AU - Metcalf SS AU - Birenz SS AU - Kunzel C AU - Wang H AU - Schrimshaw EW AU - Marshall SE FA - Northridge, Mary E FA - Metcalf, Sara S FA - Birenz, Shirley S FA - Kunzel, Carol FA - Wang, Hua FA - Schrimshaw, Eric W FA - Marshall, Stephen E TI - The Impact of Medicaid Expansion on Oral Health Equity for Older Adults: A Systems Perspective. SO - Journal of the California Dental Association. 43(7):369-77, 2015 Jul. AS - J Calif Dent Assoc. 43(7):369-77, 2015 Jul. NJ - Journal of the California Dental Association AB - This paper uses a collaborative, interdisciplinary systems science inquiry to explore implications of Medicaid expansion on achieving oral health equity for older adults. Through an iterative modeling process oriented toward the experiences of both patients and oral health care providers, complex feedback mechanisms for promoting oral health equity are articulated that acknowledge the potential for stigma as well as disparities in oral health care accessibility. Multiple factors mediate the impact of Medicaid expansion on oral health equity. IL - 1043-2256 PT - Journal Article LG - English <4. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26775347 AU - Xia X AU - Ji T AU - Liu R AU - Weng Y AU - Fang Y AU - Wang Z AU - Xu H FA - Xia, X FA - Ji, T FA - Liu, R FA - Weng, Y FA - Fang, Y FA - Wang, Z FA - Xu, H TI - Cytoplasmic p21 is responsible for paclitaxel resistance in ovarian cancer A2780 cells. SO - European Journal of Gynaecological Oncology. 36(6):662-6, 2015. AS - Eur J Gynaecol Oncol. 36(6):662-6, 2015. NJ - European journal of gynaecological oncology AB - PURPOSE: P21 which bound to cyclin-dependent kinase complexes was originally described as a suppressor of cancer cell prolifera- tion, while many recent studies have shown p21, when accumulated in the cell cytoplasm, could promote tumor progression. This study was conducted to investigate the role of p21 in the paclitaxel (PTX) resistance of ovarian cancer. AB - MATERIALS AND METHODS: Regulation of cytoplasmic p21 was performed through transfection of Akt2 constitutively active vector, Akt2 shRNA and p21 siRNA in the ovar- ian cancer cell line A2780. Akt2, p-Akt, and p21 expression were examined by Western blot and cell apoptosis rates were assessed by flow cytometry after treatment with PTX. AB - RESULTS: Induction of p21 translocation into the cytoplasm via constitutively active Akt2 transfection in A2780 enhanced the resistance to PTX, while inhibition of p21 translocation into the cytoplasm via Akt2 shRNA trans- fection in A2780 cells significantly increased PTX treatment sensitivity. Furthermore, knockdown of cytoplasmic p21 by direct p21 siRNA transfection in Akt2 overexpressed A2780 cells notably increased PTX-induced apoptosis. AB - CONCLUSION: Cytoplasmic p21 may represent a potential therapeutic target for ovarian tumors that are resistant to PTX treatment. IL - 0392-2936 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. LG - English <5. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26767271 AU - Yuan MZ AU - Wang JS AU - He LC AU - Tian KQ AU - Peng XW FA - Yuan, Mei-zhi FA - Wang, Jia-song FA - He, Liang-cai FA - Tian, Ke-qing FA - Peng, Xiao-wu TI - [Effect of schistosomiasis control in Jingzhou City from 2009 to 2013]. [Chinese] SO - Chinese Journal of Schistosomiasis Control. 27(4):422-4, 2015 Aug. AS - Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 27(4):422-4, 2015 Aug. NJ - Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control AB - OBJECTIVE: To evaluate the effect of schistosomiasis control in Jingzhou City of Hubei Province from 2009 to 2013, so as to provide the reference for the establishment of control strategy in the future. AB - METHODS: The annual reports on schistosomiasis control from 2009 to 2013 in nine counties (cities, districts) in Jingzhou City were collected and analyzed sta- tistically. AB - RESULTS: The estimated number of schistosomiasis patients in Jingzhou City dropped from 78 903 in 2009 to 28 034 in 2013, with a reduction rate of 63.37%. The schistosome infection rate of residents dropped from 2.21% in 2009 to 0.65% in 2013, which showed an exponential decline trend (y = 3.196e(-0.317x)). The number of cattle with schistosomiasis decreased from 2 132 in 2009 to 0 in 2013, and the infection rate of cattle dropped from 1.87% in 2009 to 0% in 2013. Only 2 cases with acute infection of schistosome were found in 2009, and no schistosome infected snails were found out in 2 consecutive years, 2012 and 2013. AB - CONCLUSIONS: The endemic level of schistosomiasis in Jingzhou City has decreased significantly. In order to consolidate the achievement of control, the comprehensive strategy including infection source control, snail control with mol- luscicides and snail surveillance should be implemented. IL - 1005-6661 PT - English Abstract PT - Journal Article LG - Chinese <6. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26767262 AU - He JC AU - Zhang SQ AU - Wang TP AU - Zhu LQ AU - Zhang GH AU - Wang H AU - Gao FH AU - Yang WP AU - Cao JR AU - Yin XM AU - Liu YP AU - Zhou L AU - Zhang LS AU - Wang FF AU - Hu MC AU - Si WM AU - Ding SJ AU - Xu XJ FA - He, Jia-chang FA - Zhang, Shi-qing FA - Wang, Tian-ping FA - Zhu, Liang-qiang FA - Zhang, Gong-hua FA - Wang, Hao FA - Gao, Feng-hua FA - Yang, Wei-ping FA - Cao, Jin-rong FA - Yin, Xiao-mei FA - Liu, Yi-ping FA - Zhou, Li FA - Zhang, Le-sheng FA - Wang, Feng-feng FA - Hu, Ming-chuang FA - Si, Wu-min FA - Ding, Song-jun FA - Xu, Xiao-juan TI - [Investigation on endemic situation of schistosomiasis in infection-controlled regions in Anhui Province]. [Chinese] SO - Chinese Journal of Schistosomiasis Control. 27(4):390-4, 2015 Aug. AS - Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 27(4):390-4, 2015 Aug. NJ - Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control AB - OBJECTIVE: To understand the epidemic situation of schistosomiasis in 27 counties (cities, districts) that reached the criteria of schistosomiasis infection controlled in Anhui Province. AB - METHODS: According to the requirement of The National Assessment Scheme of Schistosomiasis, 81 administrative villages where the schistosomiasis epidemic situation was relatively heaver in above-mentioned 27 counties (1 village per town, 3 towns per county) were sampled and investigated. AB - RESULTS: From 2012 to 2014, 81 villages were investigated, and 34,293 residents received the serum examinations, and 1,086 were positive with a positive rate of 3.17% (0.65%-9.58%), and the positives received stool examinations and the average stool positive rate was 0.37% (0-4.0%). The calculated prevalence of human infection was 0.01%. A total of 3 057 domestic animals were investigated including 438 cattle, 2,550 sheep, and 69 other animals, and no infections were detected. A total of 11,261 living Oncomelania hupensis snails were collected and detected, but no schistosome infected snails were found. Before this investigation, no infected snails were detected for more than 2 years [average 2.3 (2-6) years], and no acute schistosome infection cases were found for more than 2 years [average 4.9 (2-9) years]. AB - CONCLUSION: The infection rates of schistosomiasis in residents and domestic animals are relatively low, and no schistosome infected snails are found in the regions. IL - 1005-6661 PT - English Abstract PT - Journal Article LG - Chinese <7. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26767256 AU - Wang ZY AU - Zhang YG AU - Jiang L AU - Li M AU - Zhu M AU - Cal Li FA - Wang, Zhen-yu FA - Zhang, Yao-guang FA - Jiang, Li FA - Li, Mei FA - Zhu, Min FA - Cal Li TI - [Laboratory analysis and diagnosis of one transfusion-transmitted quartan malaria case in Shanghai City]. [Chinese] SO - Chinese Journal of Schistosomiasis Control. 27(4):362-6, 2015 Aug. AS - Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 27(4):362-6, 2015 Aug. NJ - Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control AB - OBJECTIVE: To diagnose a case of quartan malaria with unknown origin by laboratory detection. AB - METHODS: The clinical data of the case were collected and the epidemiological survey was conducted. The blood samples of the patient and the blood donor were detected by microscopy, rapid diagnostic test (RDT) and nested PCR, and the positive results were sequenced. AB - RESULTS: The patient did not visit malaria endemic areas and have no history of malaria infection, but have history of massive blood transfusion in surgical operation. The patient was confirmed as quartan malaria by microscopy with peripheral blood smears. The patient was transfused from three donors whose peripheral blood was none of malaria infection by microscopy, RDT and nested PCR. But sequence analysis showed that the amplified band of one donor was 100% homology to the patient' s with the improved nest-multi PCR examination. AB - CONCLUSION: This patient is confirmed of Plasmodium malariae infection via blood transfusion. Laboratory analysis and diagnosis of undefined malaria cases require multiple methods, and the improved nest-multi PCR could effectively detect the low parasitized malaria infection. IL - 1005-6661 PT - Case Reports PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - Chinese <8. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26767255 AU - Li BG AU - Chen F AU - Li WB AU - Yang H AU - Duan YC AU - Wang HY AU - Mu LX AU - Tian SH AU - Li P AU - Chen SR AU - Luo JJ FA - Li, Bing-gui FA - Chen, Feng FA - Li, Wen-bao FA - Yang, Hui FA - Duan, Yu-chun FA - Wang, Hai-ying FA - Mu, Liang-xian FA - Tian, Shu-hni FA - Li, Ping FA - Chen, Shao-rong FA - Luo, Jia-jun TI - [Research of freely grazing and wild feces behaviors in schistosomiasis endemic areas of plateau mountain area in Yunnan Province]. [Chinese] SO - Chinese Journal of Schistosomiasis Control. 27(4):353-8, 2015 Aug. AS - Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 27(4):353-8, 2015 Aug. NJ - Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control AB - OBJECTIVE: To understand the situation of the freely grazing and wild feces behaviors of residents in plateau mountain area of schistosomiasis endemic areas in Yunnan Province. AB - METHODS: Two villages of Xidian and Moguang in Heqing County, Yunnan Province were selected as the study area and the questionnaire surveys were performed to the randomly selected villagers aged 6 to 65 years with the sampling ratio of 30%. Then the respondents were tested for the infection of schistosomiasis by indirect hemagglutination assay (IHA). AB - RESULTS: Totally 412 residents were surveyed. In all the responds, the ratios of "captive breed", "freely grazing "unknown" and "no response" were 55.34%, 4.85%, 26.94% and 12.86% respectively; and the ratios of "no grazing", "less than 5/week" and "no less than 5/week" were 75.49%, 16.02% and 8.50% respectively; and the ratios of "no wild feces", "less than 5/week", "no less than 5/week" and "no answer" were 68.45%, 27.67%, 2.91% and 0.97% respectively. AB - CONCLUSIONS: Freely grazing and wild feces behaviors of residents in schistosomiasis endemic area of Heqing County are widespread, could heavily affect the control of schistosomiasis in Heqing County. The further work is to strengthen the management of human and animal feces and grazing and consolidate the results of the prevention and control of schistosomiasis and finally achieve the aim of the transmission interruption. IL - 1005-6661 PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - Chinese <9. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26749841 AU - Zhang L AU - Tu ZC AU - Yuan T AU - Ma H AU - Niesen DB AU - Wang H AU - Seeram NP FA - Zhang, Lu FA - Tu, Zong-cai FA - Yuan, Tao FA - Ma, Hang FA - Niesen, Daniel B FA - Wang, Hui FA - Seeram, Navindra P TI - New Gallotannin and other Phytochemicals from Sycamore Maple (Acer pseudoplatanus) Leaves. SO - Natural Product Communications. 10(11):1977-80, 2015 Nov. AS - Nat Prod Commun. 10(11):1977-80, 2015 Nov. NJ - Natural product communications AB - The maple (Acer) genus is a reported source of bioactive (poly)phenols, including gallotannins, but several of its members, such as the sycamore maple (A. pseudoplatanus), remain uninvestigated. Herein, thirty-nine compounds, including a new gallotannin, 1,2,3-tri-O-galloyl-6-O-(p-hydroxybenzoyl)-beta-D- glucopyranoside (1), and thirty-eight (2-39) known compounds, consisting of four gallotannins, one ellagitannin, thirteen flavonoids, eight hydroxycinnamic acids, ten benzoic acid derivatives, and two sesquiterpenoids, were isolated from sycamore maple leaves. Their structures were determined based on NMR and mass spectral analyses. The isolates were evaluated for alpha-glucosidase inhibitory and antioxidant activities. Among the isolates, the gallotannins were the most potent alpha-glucosidase inhibitors with thirteen-fold more potent activity compared with the clinical drug, acarbose (IC50 = 16-31 vs. 218 micro M). Similarly, the gallotannins showed the highest antioxidant activities, followed by the other phenolic sub-classes, while the sesquiterpenoids were inactive. IL - 1555-9475 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. LG - English <10. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26749829 AU - Kang HR AU - Eom HJ AU - Lee SR AU - Choi SU AU - Kang KS AU - Lee KR AU - Kim KH FA - Kang, Hee Rae FA - Eom, Hee Jeong FA - Lee, Seoung Rak FA - Choi, Sang Un FA - Kang, Ki Sung FA - Lee, Kang Ro FA - Kim, Ki Hyun TI - Bioassay-guided Isolation of Antiproliferative Triterpenoids from Euonymus alatus Twigs. SO - Natural Product Communications. 10(11):1929-32, 2015 Nov. AS - Nat Prod Commun. 10(11):1929-32, 2015 Nov. NJ - Natural product communications AB - Euonymus alatus (Celastraceae) has been used as an anticancer agent in Korean traditional medicine. However, the potential bioactive contributors to the anticancer effects have not been thoroughly studied. Our screening test revealed that the MeOH extract of E. alatus twigs exhibited significant cytotoxicity against A549, SK-OV-3, and SK-MEL-2 cell lines. A bioassay-guided separation of the MeOH extract of E. alatus twigs resulted in the isolation and identification of 14 triterpenes as main phytochemicals. The structures of the compounds were elucidated on the basis of spectroscopic evidence as lupeol (1), betulin (2), 3beta,28,30-lup-20(29)-ene triol (3), lupenone (4), betulone (5), 28,30-dihydroxy-3-oxolup-20(29)-ene (6), messagenin (7), glut-5-en-3beta-ol (8), maslinic acid (9), hederagenin (10), 3-oxo-11alpha-methoxyolean-12-ene (11), 3beta-hydroxy-1-oxo-olean-12-en-28-oic acid (12), ursolic acid (13), and 2a-hydroxy- ursolic acid (14). Of these compounds, 3, 6-8, and 10-14 were isolated for the first time from this plant. All isolated triterpenoids had consistent antiproliferative activities against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines. Compounds 2, 5, and 7 showed significant cytotoxicity against all four cell lines tested, with IC50 values of 3.26-8.61 micro M. IL - 1555-9475 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <11. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26731383 AU - Chang J AU - McGrory BJ AU - Rana A AU - Becker MW AU - Babikian GM AU - Guay P AU - Smith KA FA - Chang, Justin FA - McGrory, Brian J FA - Rana, Adam FA - Becker, Michael W FA - Babikian, George M FA - Guay, Peter FA - Smith, Kahsi A IN - Chang,Justin. Division of Joint Replacement Surgery, Maine Medical Center, and Maine Joint Replacement Institute, Portland, Maine. TI - Current Orthopaedic Surgeon Practices for Nonarthroplasty Treatment of Osteoarthritis of Adult Hip and Knee. SO - Journal of Surgical Orthopaedic Advances. 24(4):213-20, 2015. AS - J Surg Orthop Adv. 24(4):213-20, 2015. NJ - Journal of surgical orthopaedic advances AB - Evidence-based guidelines have recently been published for the nonarthroplasty treatment of osteoarthritis of the hip and knee and are becoming an expected part of comprehensive patient care. To understand how current treatment practices correlate with these guidelines, a survey was administered to 50 consecutive hip replacement and 50 consecutive knee replacement patients immediately before arthroplasty for osteoarthritis in one group practice. This article is a compilation of patients' usage of the modalities suggested in two such exemplary guidelines and demonstrates that patient and surgeon preferences, as well as factors such as preoperative Oxford score, body mass index, age, and joint involved, affect usage of one or more of the commonly employed preoperative modalities. This information provides orthopaedic surgeons and administrators with a compilation of responses that reflects surgeon and patient preferences for treatment before surgery. IL - 1548-825X PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <12. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26710606 AU - Tian L AU - Xu R AU - Wang Z AU - Liu N AU - Feng F AU - Qu Z FA - Tian, Li FA - Xu, Rongqi FA - Wang, Zhuanbin FA - Liu, Na FA - Feng, Fengjuan FA - Qu, Zhicai TI - [Vesicular transport protein VdSec22 is involved in secretion of extracellular protein and pathogenicity in Verticillium dahliae]. [Chinese] SO - Wei Sheng Wu Hsueh Pao - Acta Microbiologica Sinica. 55(7):873-81, 2015 Jul 4. AS - Wei Sheng Wu Hsueh Pao. 55(7):873-81, 2015 Jul 4. NJ - Wei sheng wu xue bao = Acta microbiologica Sinica AB - OBJECTIVE: The aim of this study was to characterize VdSec22 of Verticillium dahliae, which is an intracellular vesicle fusion protein involved in fungal secretory pathway, and to provide a potential gene target for controlling Verticillium wilt disease. AB - METHODS: VdSec22 deletion mutant DELTAQF and functional complementation strain CDELTAQF by reintroducing the VdSec22 intoAQF were constructed. Secretion ability of extracellular protein (including pectinase, cellulose, and phytotoxin protin) and pathogenicity of DELTAQF and CDELTAQF were studied compared with that of wild type strain Vd991. Expression level of ER molecular chaperones by quantitative PCR was also performed to infer whether ER stress was induced in DELTAQF. AB - RESULTS: We successfully constructed VdSec22 deletion mutant strain DELTAQF and functional complementation strain CDELTAQF. VdSec22 deficiencies did disturb secretion ability of extracellular protein such as pectinase, cellulose, and phytotoxin protin. Pathogenicity of DELTAQF was dramatically reduce accordingly. We also found loss of VdSec22 resulted in ER stress in V. dahliae cells. Reintroducing functional VdSec22 into DELTAQF can compensate for the deficiencies mentioned above. AB - CONCLUSION: VdSec22 is an important secretion pathway protein involved in secretion of extracellular protein and pathogenicity in V. dahliae. VdSec22 provides a potential gene target for controlling the devastating disease. IL - 0001-6209 PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - Chinese <13. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26620032 AU - Khan MH AU - Ashrafuzzaman SM AU - Taib AN AU - Alam MT AU - Khan SH AU - Goldstein SK AU - Rahman R FA - Khan, M H FA - Ashrafuzzaman, S M FA - Taib, A N FA - Alam, M T FA - Khan, S H FA - Goldstein, S K FA - Rahman, R IN - Khan,M H. Dr Mahfujul Haq Khan, Professor and Head, Department of Dentistry, Bangladesh Institute of Research & Rehabilitation in Diabetes, Endocrine & Metabolic Disorder (BIRDEM) and Ibrahim Medical College, Dhaka, Bangladesh. TI - Ambras Syndrome: First Reported Case in Bangladesh and its Oral Rehabilitation. SO - Mymensingh Medical Journal: MMJ. 24(4):859-63, 2015 Oct. AS - Mymensingh Med J. 24(4):859-63, 2015 Oct. NJ - Mymensingh medical journal : MMJ AB - People with rare hypertrichosis syndromes became crowd-drawing money-making phenomena in many 19th century sideshow acts. These individuals have been referred to as dog-men, hair-men, and werewolves. In 1993, Baumister et al. described congenital hypertrichosis lanuginose or Ambras syndrome: a distinct form of congenital hypertrichosis characterized by excessive hair growth over the body and face associated with facial and occasional dental anomalies. Much is not known about this syndrome since fewer than 50 cases have been documented worldwide. In this case report, a nine year old girl presented with excessive hair growth throughout her body that was denser along her midline. Furthermore, her face displayed the typical dysmorphic features characteristic of Ambras syndrome: a round tip nose, thickened nasal cartilage, antiverted nares, prominent philtrum with deep groove, and a trapezoid mouth. Oral examination revealed normal oral mucosa with completely missing and unerupted decidious and permanent teeth. Panoramic radiographs confirmed unerupted deciduous teeth. Previous case reports have mentioned the presence of occasional dental anomalies such as retarded first and second dentition and absence of some teeth. However, this is the first reported case of Ambras syndrome presenting with complete anodontia. Prior cytogenetic studies performed on persons with Ambras syndrome have implicated a balanced pericentric inversion of chromosome 8. However, it is likely that dental anomalies are likely a result of a different genetic rearrangement. Further studies are needed to explore the cause of this rare phenotype of Ambras syndrome with complete unerupted dentition. IL - 1022-4742 PT - Case Reports PT - Journal Article LG - English <14. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26575882 AU - He J AU - Wang HZ AU - Xu FL AU - Yang X AU - Wang R AU - Zou HY AU - Yu WZ FA - He, Jiang FA - Wang, Hui-Zhen FA - Xu, Fa-Liang FA - Yang, Xi FA - Wang, Rui FA - Zou, Hong-Yun FA - Yu, Wu-Zhong IN - He,Jiang. Institute of Clinical Medicine, Urumqi General Hospital of Lanzhou Military Region, Urumqi 830000, China. yuwz2013@126.com. TI - [Mutation analysis of the PAH gene in children with phenylketonuria from the Qinghai area of China]. [Chinese] SO - Zhongguo Dangdai Erke Zazhi. 17(11):1221-7, 2015 Nov. AS - Zhongguo Dang Dai Er Ke Za Zhi. 17(11):1221-7, 2015 Nov. NJ - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics AB - OBJECTIVE: To study the mutation characteristics of the phenylalanine hydroxylase (PAH) gene in children with phenylketonuria (PKU) from the Qinghai area of China, in order to provide basic information for genetic counseling and prenatal diagnosis. AB - METHODS: Mutations of the PAH gene were detected in the promoter and exons 1-13 and their flanking intronic sequences of PAH gene by PCR and DNA sequencing in 49 children with PKU and their parents from the Qinghai area of China. AB - RESULTS: A total of 30 different mutations were detected in 80 out of 98 mutant alleles (82%), including 19 missense (63%), 5 nonsense (17%), 3 splice-site (10%) and 3 deletions (10%). Most mutations were detected in exons 3, 6, 7, 11 and intron 4 of PAH gene. The most frequent mutations were p.R243Q (19%), IVS4-1G>A (9%), p.Y356X (7%) and p.EX6-96A>G(5%). Two novel mutations p.N93fsX5 (c.279-282delCATC) and p.G171E (c.512G>A) were found. p.H64fsX9(c.190delC) was documented for the second time in Chinese PAH gene. The mutation spectrum of the gene PAH in the Qinghai population was similar to that in other populations in North China while significantly different from that in the populations from some provinces in southern China, Japan and Europe. AB - CONCLUSIONS: The mutations of PAH gene in the Qinghai area of China demonstrate a unique diversity, complexity and specificity. IL - 1008-8830 PT - English Abstract PT - Journal Article LG - Chinese <15. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26575876 AU - Wang Z AU - Li YC AU - Chen L FA - Wang, Zhen FA - Li, Ya-Chun FA - Chen, Lu IN - Wang,Zhen. Department of Pediatrics, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 201600, China. yachunli@126.com. TI - [Early identification of refractory Mycoplasma pneumoniae pneumonia in children]. [Chinese] SO - Zhongguo Dangdai Erke Zazhi. 17(11):1189-92, 2015 Nov. AS - Zhongguo Dang Dai Er Ke Za Zhi. 17(11):1189-92, 2015 Nov. NJ - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics AB - OBJECTIVE: To investigate the clinical indicators for early identification of refractory Mycoplasma pneumoniae pneumonia (RMPP) in children. AB - METHODS: The clinical data of 142 children with Mycoplasma pneumoniae pneumonia (MPP) between January 2014 and June 2015 were retrospectively studied. Among the 142 children, there were 32 cases of RMPP and 110 cases of non-refractory MPP. The clinical data were compared between the RMPP and non-refractory MPP groups. AB - RESULTS: The percentage of school-age children in the RMPP group was higher than in the non-refractory MPP group (P<0.05). The mean onset age in the RMPP group was older than the non-refractory MPP group (P<0.05). Steroid was used in 93.8% of RMPP children compared with 7.3% of non-refractory MPP children (P<0.001). Consolidation of lung on chest X-Ray was shown in 87.5% of RMPP children compared with 42.7% of non-refractory MPP children (P<0.001). The incidence of pleural effusion in the RMPP group was higher than in the non-refractory MPP group (P<0.001). The RMPP group had higher percentages of individuals with CRP>40 mg/L and ESR>30 mm/h+LDH>300 IU/L than the non-refractory MPP group (P<0.05). AB - CONCLUSIONS: RMPP is common in school-age children. Consolidation of lung on chest X-Ray, pleural effusion and increased levels of CRP and ESR+LDH may be helpful to early identification of RMPP in children. IL - 1008-8830 PT - English Abstract PT - Journal Article LG - Chinese <16. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26572677 AU - Tremmel JA AU - Fearon WF AU - Lee BK AU - Lim HS AU - Yong AS AU - Yamada R AU - Tanaka S AU - Lee DP AU - Yeung AC FA - Tremmel, Jennifer A FA - Fearon, William F FA - Lee, Bong-Ki FA - Lim, Hong-Seok FA - Yong, Andy S FA - Yamada, Ryotaro FA - Tanaka, Shigemitsu FA - Lee, David P FA - Yeung, Alan C IN - Tremmel,Jennifer A. Division of Cardiovascular Medicine, Stanford University Medical Center, Stanford, CA. Fearon,William F. Division of Cardiovascular Medicine, Stanford University Medical Center, Stanford, CA. Lee,Bong-Ki. Division of Cardiology, Kangwon National University School of Medicine, Chuncheon, Republic of Korea. Lim,Hong-Seok. Department of Cardiology, Ajou University School of Medicine, Suwon, Republic of Korea. Yong,Andy S. Department of Cardiology, Royal Prince Alfred and Concord Hospitals, University of Sydney, Sydney, Australia. Yamada,Ryotaro. Department of Cardiology, Tokai University Oiso Hospital, Oiso, Japan. Tanaka,Shigemitsu. Department of Cardiology, Kawasaki Medical School, Kurashiki, Japan. Lee,David P. Division of Cardiovascular Medicine, Stanford University Medical Center, Stanford, CA. Yeung,Alan C. Division of Cardiovascular Medicine, Stanford University Medical Center, Stanford, CA. TI - Response to Letters Regarding Article, "Invasive Evaluation of Patients With Angina in the Absence of Obstructive Coronary Artery Disease". SO - Circulation. 132(20):e244, 2015 Nov 17. AS - Circulation. 132(20):e244, 2015 Nov 17. NJ - Circulation LM - Available online from MWHC library: 1950 - present, Available in print through MWHC library: 1999 - 2006 IL - 0009-7322 DO - http://dx.doi.org/10.1161/CIRCULATIONAHA.115.017741 PT - Comment PT - Letter LG - English <17. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26554809 AU - Wang H AU - Zhou Y AU - Ou L AU - Li C AU - Liu J AU - Xiang L FA - Wang, Hongwei FA - Zhou, Yue FA - Ou, Lan FA - Li, Changqing FA - Liu, Jun FA - Xiang, Liangbi IN - Wang,Hongwei. From the Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang, Liaoning (HW, JL, LX); Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing (YZ, CL); and Department of Radiology, Southwest Hospital, Third Military Medical University, Chongqing, China (LO). TI - Traumatic Vertebral Fractures and Concomitant Fractures of the Rib in Southwest China, 2001 to 2010: An Observational Study. SO - Medicine. 94(44):e1985, 2015 Nov. AS - Medicine (Baltimore). 94(44):e1985, 2015 Nov. NJ - Medicine LM - Available online from MWHC library: 1995 - present, Available in print through MWHC library: 1999 - 2006 AB - To our knowledge, the clinical characteristics of traumatic vertebral fractures and concomitant fractures of the rib (TVF-RF) have not been described in previous studies.To investigate the clinical characteristics of patients managed for TVF-RF. A retrospective study of 3142 patients who presented with traumatic vertebral fractures was performed. Two hundred twenty-six patients (7.2%) suffered from TVF-RF.Incidence rate ratios were then calculated with respect to the level of injury to the spine, the ASIA classification of neurological deficits and age.There were 171 male (75.7%) and 55 female (24.3%) patients with a mean age of 43.8 years. The most common mechanisms were falls from high heights in 81 cases and road traffic crashes in 67 cases. Right-sided rib injury occurred in 106 cases, left-sided injury occurred in 76 cases, and bilateral injury occurred in 44 cases. The most frequent location of the rib fractures was from the fourth rib to the ninth rib (70.3%, 510/725). Initial pulmonary complications (IPC) after trauma occurred in 116 cases (51.3%). The mortality rate for the entire group was 1.3% (3/226). The patients with thoracic vertebral fractures and neurological deficits had a higher frequency of multiple rib fractures and IPC than the other patients (P < 0.05). With the increased number of rib fractures, the frequency of IPC and mean intensive care unit (ICU) length of stay also increased.The rates of complications for patients with rib fractures were significantly different from those without rib fractures. We should pay much attention to the patients who presented with thoracic vertebral fractures and neurological deficits for minimizing further complications and mortality in such patients who had a higher frequency of multiple rib fractures and IPC than the other patients. IL - 0025-7974 DO - http://dx.doi.org/10.1097/MD.0000000000001985 PT - Journal Article PT - Multicenter Study PT - Observational Study PT - Research Support, Non-U.S. Gov't LG - English <18. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26554804 AU - Chen IW AU - Yang HM AU - Chiu CH AU - Yeh JT AU - Huang CH AU - Huang YY FA - Chen, I-Wen FA - Yang, Hui-Mei FA - Chiu, Cheng-Hsun FA - Yeh, Jiun-Ting FA - Huang, Chung-Huei FA - Huang, Yu-Yao IN - Chen,I-Wen. From the Division of Endocrinology and Metabolism, Department of Internal Medicine (I-WC, H-MY, C-HH, Y-YH), Molecular Infectious Disease Research Center, Division of Pediatric Infectious Diseases, Department of Pediatrics (C-HC); and Division of Trauma Plastic Surgery, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan City, Taiwan (J-TY). TI - Clinical Characteristics and Risk Factor Analysis for Lower-Extremity Amputations in Diabetic Patients With Foot Ulcer Complicated by Necrotizing Fasciitis. SO - Medicine. 94(44):e1957, 2015 Nov. AS - Medicine (Baltimore). 94(44):e1957, 2015 Nov. NJ - Medicine LM - Available online from MWHC library: 1995 - present, Available in print through MWHC library: 1999 - 2006 AB - Patients with diabetes are at a higher risk of having diabetic foot ulcers (DFUs) or necrotizing fasciitis (NF). The present study aims to examine the clinical characteristics and associated risk factors for lower-extremity amputation (LEA) in patients with DFU complicated by NF.We retrospectively reviewed patients treated at a major diabetic foot center in Taiwan between 2009 and 2014. Of the 2265 cases 110 had lower-extremity NF. Limb preservation outcomes were classified as major LEA, minor LEA, or limb-preserved. Clinical characteristics, laboratory data, and bacterial culture results were collected for analysis.Of the 110 patients with NF, 100 had concomitant DFUs (NF with DFU) and the remaining 10 had no DFU (NF without DFU). None of the NF patients without DFU died nor had their leg amputated. Two NF patients with DFU died of complications. The amputation rate in the surviving 98 NF patients with DFU was 72.4% (46.9% minor LEA and 25.5% major LEA). Seventy percent of the NF patients without DFU had monomicrobial infections (60% with Streptococcus species), and 81.4% NF patients with DFU had polymicrobial infections. Anaerobic organisms were identified in 66% of the NF patients with DFU. Multinomial logistic regression analysis revealed an association between high-grade Wagner wound classification (Wagner 4 and Wagner 5) and LEA (adjusted odds ratio [aOR] = 21.856, 95% confidence interval [95% CI] = 1.625-203.947, P = 0.02 and aOR = 20.094, 95% CI = 1.968-205.216, P = 0.01 for major and minor LEA, respectively) for NF patients with DFU. In addition, a lower serum albumin level was associated with major LEA (OR = 0.066, P = 0.002).In summary, once DFUs were complicated by NF, the risk of amputation increased. Empirical treatment for NF patients with DFU should cover polymicrobial infections, including anaerobic organisms. The high-grade wound classification and low serum albumin level were associated with LEA. IL - 0025-7974 DO - http://dx.doi.org/10.1097/MD.0000000000001957 PT - Journal Article PT - Observational Study PT - Research Support, Non-U.S. Gov't LG - English <19. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26554802 AU - Yang JH AU - Huang PY AU - Shie SS AU - Yang S AU - Tsao KC AU - Wu TL AU - Leu HS AU - Huang CT FA - Yang, Jeng-How FA - Huang, Po-Yen FA - Shie, Shian-Sen FA - Yang, Shuan FA - Tsao, Kuo-Chien FA - Wu, Tsu-Lan FA - Leu, Hsieh-Shong FA - Huang, Ching-Tai IN - Yang,Jeng-How. From the Division of Infectious Diseases, Department of Medicine (J-HY, P-YH, S-SS, H-SL, C-TH); and Department of Laboratory Medicine, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan (YS, K-CT, T-LW). TI - Predictive Symptoms and Signs of Laboratory-confirmed Influenza: A Prospective Surveillance Study of Two Metropolitan Areas in Taiwan. SO - Medicine. 94(44):e1952, 2015 Nov. AS - Medicine (Baltimore). 94(44):e1952, 2015 Nov. NJ - Medicine LM - Available online from MWHC library: 1995 - present, Available in print through MWHC library: 1999 - 2006 AB - Influenza infection poses annual threats and leads to significant morbidity and mortality. Early diagnosis is the key to successful treatment. Laboratory-based diagnosis has various limitations. Diagnosis based on symptoms or signs is still indispensable in clinical practice. We investigated the symptoms or signs associated with laboratory-confirmed influenza.A prospective study across 2 influenza seasons was performed from June 2010 to June 2012 at 2 branches (Taipei and Lin-Kou) of Chang Gung Memorial Hospital. Patients who visited outpatient clinics with suspected acute respiratory tract infection were sampled by throat swab or nasopharyngeal swab. RT-PCR and/or virus culture were used as a reference standard. We used logistic regression to identify the symptoms or signs associated with laboratory-confirmed influenza infection. We also evaluated the performance metrics of different influenza-like illness used in Taiwan, the USA, and WHO.A total of 158 patients were included in the study. The prevalence of influenza infection was 45% (71/158). Fever, cough, rhinorrhea, sneezing, and nasal congestion were significant predictors for influenza infection. Whereas fever + cough had a best sensitivity (86%; confidence interval [CI] 76%-93%), fever + cough and sneezing had a best specificity (77%; CI 62%-88%). Different case definitions of influenza-like illness had comparable accuracy in sensitivity and specificity.Clinical diagnosis based on symptoms and signs is useful for allocating resources, identifying those who may benefit from early antiviral therapy and providing valuable information for surveillance purpose. IL - 0025-7974 DO - http://dx.doi.org/10.1097/MD.0000000000001952 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <20. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26232442 AU - Wang G AU - Jung K AU - Winnenburg R AU - Shah NH FA - Wang, Guan FA - Jung, Kenneth FA - Winnenburg, Rainer FA - Shah, Nigam H IN - Wang,Guan. Stanford University, Center for Biomedical Informatics, Stanford, California, USA guanw@stanford.edu. Jung,Kenneth. Stanford University, Center for Biomedical Informatics, Stanford, California, USA guanw@stanford.edu. Winnenburg,Rainer. Stanford University, Center for Biomedical Informatics, Stanford, California, USA guanw@stanford.edu. Shah,Nigam H. Stanford University, Center for Biomedical Informatics, Stanford, California, USA guanw@stanford.edu. TI - A method for systematic discovery of adverse drug events from clinical notes. SO - Journal of the American Medical Informatics Association. 22(6):1196-204, 2015 Nov. AS - J Am Med Inform Assoc. 22(6):1196-204, 2015 Nov. NJ - Journal of the American Medical Informatics Association : JAMIA LM - Available online through MWHC library: 2003 - present, Available in print through MWHC library: 1999 - present AB - OBJECTIVE: Adverse drug events (ADEs) are undesired harmful effects resulting from use of a medication, and occur in 30% of hospitalized patients. The authors have developed a data-mining method for systematic, automated detection of ADEs from electronic medical records. AB - MATERIALS AND METHODS: This method uses the text from 9.5 million clinical notes, along with prior knowledge of drug usages and known ADEs, as inputs. These inputs are further processed into statistics used by a discriminative classifier which outputs the probability that a given drug-disorder pair represents a valid ADE association. Putative ADEs identified by the classifier are further filtered for positive support in 2 independent, complementary data sources. The authors evaluate this method by assessing support for the predictions in other curated data sources, including a manually curated, time-indexed reference standard of label change events. AB - RESULTS: This method uses a classifier that achieves an area under the curve of 0.94 on a held out test set. The classifier is used on 2,362,950 possible drug-disorder pairs comprised of 1602 unique drugs and 1475 unique disorders for which we had data, resulting in 240 high-confidence, well-supported drug-AE associations. Eighty-seven of them (36%) are supported in at least one of the resources that have information that was not available to the classifier. AB - CONCLUSION: This method demonstrates the feasibility of systematic post-marketing surveillance for ADEs using electronic medical records, a key component of the learning healthcare system.Copyright © The Author 2015. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com. IL - 1067-5027 DO - http://dx.doi.org/10.1093/jamia/ocv102 PT - Evaluation Studies PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Validation Studies LG - English <21. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26142422 AU - Shirts BH AU - Salama JS AU - Aronson SJ AU - Chung WK AU - Gray SW AU - Hindorff LA AU - Jarvik GP AU - Plon SE AU - Stoffel EM AU - Tarczy-Hornoch PZ AU - Van Allen EM AU - Weck KE AU - Chute CG AU - Freimuth RR AU - Grundmeier RW AU - Hartzler AL AU - Li R AU - Peissig PL AU - Peterson JF AU - Rasmussen LV AU - Starren JB AU - Williams MS AU - Overby CL FA - Shirts, Brian H FA - Salama, Joseph S FA - Aronson, Samuel J FA - Chung, Wendy K FA - Gray, Stacy W FA - Hindorff, Lucia A FA - Jarvik, Gail P FA - Plon, Sharon E FA - Stoffel, Elena M FA - Tarczy-Hornoch, Peter Z FA - Van Allen, Eliezer M FA - Weck, Karen E FA - Chute, Christopher G FA - Freimuth, Robert R FA - Grundmeier, Robert W FA - Hartzler, Andrea L FA - Li, Rongling FA - Peissig, Peggy L FA - Peterson, Josh F FA - Rasmussen, Luke V FA - Starren, Justin B FA - Williams, Marc S FA - Overby, Casey L IN - Shirts,Brian H. Department of Laboratory Medicine, University of Washington, Seattle, WA, 98195, USA shirtsb@uw.edu. Salama,Joseph S. Department of Medicine, Division of Medical Genetics, University of Washington, Seattle, WA, USA. Aronson,Samuel J. Personalized Medicine, Partners Healthcare, Boston, MA, USA. Chung,Wendy K. Department of Pediatrics, Columbia University Medical Center, New York, NY, USA. Gray,Stacy W. Department of Medicine, Harvard Medical School, Boston, MA, USA Dana-Farber Cancer Institute, Boston, MA, USA. Hindorff,Lucia A. National Human Genome Research Institute, NIH, Rockville, MD, USA. Jarvik,Gail P. Department of Medicine, Division of Medical Genetics, University of Washington, Seattle, WA, USA Department of Genome Sciences, University of Washington, Seattle, WA, USA. Plon,Sharon E. Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA. Stoffel,Elena M. Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA. Tarczy-Hornoch,Peter Z. Department of Biomedical Informatics and Medical Education, University of Washington, Seattle, WA, USA. Van Allen,Eliezer M. Dana-Farber Cancer Institute, Boston, MA, USA The Broad Institute of MIT and Harvard, Cambridge, MA, USA. Weck,Karen E. Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Chute,Christopher G. Department of Health Sciences Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, USA. Freimuth,Robert R. Department of Health Sciences Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, USA. Grundmeier,Robert W. Department of Biomedical and Health Informatics, The Children's Hospital of Philadelphia, Philadelphia, PA, USA. Hartzler,Andrea L. Group Health Research Institute, Group Health Cooperative, Seattle, WA, USA. Li,Rongling. National Human Genome Research Institute, NIH, Rockville, MD, USA. Peissig,Peggy L. Biomedical Informatics Research Center, Marshfield Clinic Research Foundation, Marshfield, WI, USA. Peterson,Josh F. Department of Biomedical Informatics, Vanderbilt, Nashville, TN, USA. Rasmussen,Luke V. Department of Preventive Medicine, Division of Health and Biomedical Informatics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. Starren,Justin B. Department of Preventive Medicine, Division of Health and Biomedical Informatics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. Williams,Marc S. Genome Medicine Institute, Geisinger Medical Center, Danville, PA, USA. Overby,Casey L. Genome Medicine Institute, Geisinger Medical Center, Danville, PA, USA Department of Medicine, Program for Personalized and Genomic Medicine and Center for Health-Related Informatics and Bioimaging, University of Maryland School of Medicine, Baltimore, MD, USA. TI - CSER and eMERGE: current and potential state of the display of genetic information in the electronic health record. SO - Journal of the American Medical Informatics Association. 22(6):1231-42, 2015 Nov. AS - J Am Med Inform Assoc. 22(6):1231-42, 2015 Nov. NJ - Journal of the American Medical Informatics Association : JAMIA LM - Available online through MWHC library: 2003 - present, Available in print through MWHC library: 1999 - present AB - OBJECTIVE: Clinicians' ability to use and interpret genetic information depends upon how those data are displayed in electronic health records (EHRs). There is a critical need to develop systems to effectively display genetic information in EHRs and augment clinical decision support (CDS). AB - MATERIALS AND METHODS: The National Institutes of Health (NIH)-sponsored Clinical Sequencing Exploratory Research and Electronic Medical Records & Genomics EHR Working Groups conducted a multiphase, iterative process involving working group discussions and 2 surveys in order to determine how genetic and genomic information are currently displayed in EHRs, envision optimal uses for different types of genetic or genomic information, and prioritize areas for EHR improvement. AB - RESULTS: There is substantial heterogeneity in how genetic information enters and is documented in EHR systems. Most institutions indicated that genetic information was displayed in multiple locations in their EHRs. Among surveyed institutions, genetic information enters the EHR through multiple laboratory sources and through clinician notes. For laboratory-based data, the source laboratory was the main determinant of the location of genetic information in the EHR. The highest priority recommendation was to address the need to implement CDS mechanisms and content for decision support for medically actionable genetic information. AB - CONCLUSION: Heterogeneity of genetic information flow and importance of source laboratory, rather than clinical content, as a determinant of information representation are major barriers to using genetic information optimally in patient care. Greater effort to develop interoperable systems to receive and consistently display genetic and/or genomic information and alert clinicians to genomic-dependent improvements to clinical care is recommended.Copyright Published by Oxford University Press on behalf of the American Medical Informatics Association 2015. This work is written by US Government employees and is in the public domain in the US. IL - 1067-5027 DO - http://dx.doi.org/10.1093/jamia/ocv065 PT - Journal Article PT - Research Support, N.I.H., Extramural LG - English <22. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25829460 AU - Payne TH AU - Hines LE AU - Chan RC AU - Hartman S AU - Kapusnik-Uner J AU - Russ AL AU - Chaffee BW AU - Hartman C AU - Tamis V AU - Galbreth B AU - Glassman PA AU - Phansalkar S AU - van der Sijs H AU - Gephart SM AU - Mann G AU - Strasberg HR AU - Grizzle AJ AU - Brown M AU - Kuperman GJ AU - Steiner C AU - Sullins A AU - Ryan H AU - Wittie MA AU - Malone DC FA - Payne, Thomas H FA - Hines, Lisa E FA - Chan, Raymond C FA - Hartman, Seth FA - Kapusnik-Uner, Joan FA - Russ, Alissa L FA - Chaffee, Bruce W FA - Hartman, Christian FA - Tamis, Victoria FA - Galbreth, Brian FA - Glassman, Peter A FA - Phansalkar, Shobha FA - van der Sijs, Heleen FA - Gephart, Sheila M FA - Mann, Gordon FA - Strasberg, Howard R FA - Grizzle, Amy J FA - Brown, Mary FA - Kuperman, Gilad J FA - Steiner, Chris FA - Sullins, Amanda FA - Ryan, Hugh FA - Wittie, Michael A FA - Malone, Daniel C IN - Payne,Thomas H. Department of Medicine, University of Washington, Seattle, WA, USA. Hines,Lisa E. Department of Pharmacy Practice & Science, The University of Arizona College of Pharmacy, Tucson, AZ, USA. Chan,Raymond C. Information Technology, Sentara Healthcare, Virginia Beach, VA, USA. Hartman,Seth. Department of Pharmacy Services, Oregon Health & Science University, Portland, OR, USA. Kapusnik-Uner,Joan. Clinical Editorial, FDB (First Databank, Inc.), San Francisco, CA, USA. Russ,Alissa L. Center for Health Information and Communication, Department of Veterans Affairs, Veterans Health Administration, Health Services Research and Development Service, Richard L. Roudebush VA Medical Center; Regenstrief Institute, Inc. Indianapolis, IN USA; and Purdue University College of Pharmacy, West Lafayette, IN USA. Chaffee,Bruce W. Department of Pharmacy, The University of Michigan Health System, Ann Arbor, MI, USA. Hartman,Christian. Clinical Solutions, Pharmacy OneSource, Boston, MA, USA. Tamis,Victoria. PeaceHealth St. John Medical Center, Longview WA, USA. Galbreth,Brian. At the time of this study was with Pharmacy Services, PeaceHealth Southwest Medical Center, Vancouver, WA, USA. Glassman,Peter A. Internal Medicine, Department of Veterans Affairs (VA), Greater Los Angeles Healthcare System, Los Angeles, CA, USA. Phansalkar,Shobha. Medical Informatics, Wolters Kluwer Health - Clinical Solutions, Newton, MA, USA/San Diego, CA, USA. van der Sijs,Heleen. Department of Hospital Pharmacy, Erasmus Medical Center, Rotterdam, Netherlands. Gephart,Sheila M. College of Nursing, The University of Arizona, Tucson, AZ, USA. Mann,Gordon. Pharmacy, Epic, Verona, WI, USA. Strasberg,Howard R. Medical Informatics, Wolters Kluwer Health - Clinical Solutions, Newton, MA, USA/San Diego, CA, USA. Grizzle,Amy J. Center for Health Outcomes & PharmacoEconomic Research, The University of Arizona College of Pharmacy, Tucson, AZ, USA. Brown,Mary. Department of Pharmacy Practice & Science, The University of Arizona College of Pharmacy, Tucson, AZ, USA. Kuperman,Gilad J. Interoperability Informatics, New York-Presbyterian Hospital, New York, NY, USA. Steiner,Chris. Editorial Systems, Gold Standard Drug Databases/Elsevier, Tampa, FL, USA. Sullins,Amanda. Information Technology and Services, Cerner Corporation, Kansas City, MO, USA. Ryan,Hugh. Information Technology and Services, Cerner Corporation, Kansas City, MO, USA. Wittie,Michael A. Office of Clinical Quality and Safety, Office of the National Coordinator for Health Information Technology, Department of Health and Human Services, Washington, DC, USA. Malone,Daniel C. Department of Pharmacy Practice & Science, The University of Arizona College of Pharmacy, Tucson, AZ, USA malone@pharmacy.arizona.edu. TI - Recommendations to improve the usability of drug-drug interaction clinical decision support alerts. [Review] SO - Journal of the American Medical Informatics Association. 22(6):1243-50, 2015 Nov. AS - J Am Med Inform Assoc. 22(6):1243-50, 2015 Nov. NJ - Journal of the American Medical Informatics Association : JAMIA LM - Available online through MWHC library: 2003 - present, Available in print through MWHC library: 1999 - present AB - OBJECTIVE: To establish preferred strategies for presenting drug-drug interaction (DDI) clinical decision support alerts. AB - MATERIALS AND METHODS: A DDI Clinical Decision Support Conference Series included a workgroup consisting of 24 clinical, usability, and informatics experts representing academia, health information technology (IT) vendors, healthcare organizations, and the Office of the National Coordinator for Health IT. Workgroup members met via web-based meetings 12 times from January 2013 to February 2014, and two in-person meetings to reach consensus on recommendations to improve decision support for DDIs. We addressed three key questions: (1) what, how, where, and when do we display DDI decision support? (2) should presentation of DDI decision support vary by clinicians? and (3) how should effectiveness of DDI decision support be measured? AB - RESULTS: Our recommendations include the consistent use of terminology, visual cues, minimal text, formatting, content, and reporting standards to facilitate usability. All clinicians involved in the medication use process should be able to view DDI alerts and actions by other clinicians. Override rates are common but may not be a good measure of effectiveness. AB - DISCUSSION: Seven core elements should be included with DDI decision support. DDI information should be presented to all clinicians. Finally, in their current form, override rates have limited capability to evaluate alert effectiveness. AB - CONCLUSION: DDI clinical decision support alerts need major improvements. We provide recommendations for healthcare organizations and IT vendors to improve the clinician interface of DDI alerts, with the aim of reducing alert fatigue and improving patient safety.Copyright © The Author 2015. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com. IL - 1067-5027 DO - http://dx.doi.org/10.1093/jamia/ocv011 PT - Consensus Development Conference PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PT - Review LG - English <23. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26256934 AU - Park AE AU - Sutton ER AU - Heniford BT FA - Park, Adrian E FA - Sutton, Erica R H FA - Heniford, B Todd IN - Park,Adrian E. Department of Surgery, Anne Arundel Medical Center, Johns Hopkins University School of Medicine, Annapolis, MD. Electronic address: apark@aahs.org. Sutton,Erica R H. Department of Surgery, University of Maryland School of Medicine, Baltimore, MD. Heniford,B Todd. Department of Surgery, Carolinas Medical Center, Charlotte, NC. TI - Minimally invasive surgery fellowship graduates: Their demographics, practice patterns, and contributions. SO - Surgery. 158(6):1462-7, 2015 Dec. AS - Surgery. 158(6):1462-7, 2015 Dec. NJ - Surgery LM - Available online from MWHC library: 1995 - present, Available in print through MWHC library: 1999 - 2006 AB - BACKGROUND: Fellowship opportunities in minimally invasive surgery, bariatric, gastrointestinal, and hepatobiliary arose to address unmet training needs. The large cohort of non-Accreditation Council for Graduate Medical Education -accredited fellowship graduates (NACGMEG) has been difficult to track. In this, the largest survey of graduates to date, our goal was to characterize this unique group's demographics and professional activities. AB - STUDY DESIGN: A total of 580 NACGMEG were surveyed covering 150 data points: demographics, practice patterns, academics, lifestyle, leadership, and maintenance of certification. AB - RESULTS: Of 580 previous fellows, 234 responded. Demographics included: average age 37 years, 84% male, 75% in urban settings, 49% in purely academic practice, and 58% in practice <5 years. They averaged 337 operating room cases/year (approximately 400/year for private practice vs 300/year for academic). NACGMEG averaged 100 flexible endoscopies/year (61 esophagogastroduodenoscopies, 39 colon). In the past 24 months, 60% had submitted abstracts to a national meeting, and 54% submitted manuscripts to peer-reviewed journals. Subset analyses revealed relevant relationships. There was high satisfaction (98%) that their fellowship experience met expectations; 78% termed their fellowships, versus 50% for residencies, highly pertinent to their current practices. 63% of previous fellows occupy local leadership roles, and most engage in maintenance of certification activities. AB - CONCLUSION: Fellowship alumnae appear to be productive contributors to American surgery. They are clinically and academically active, believe endoscopy is important, have adopted continuous learning, and most assume work leadership roles. The majority acknowledge their fellowship training as having met expectations and uniquely equipping them for their current practice.Copyright © 2015 Elsevier Inc. All rights reserved. IL - 0039-6060 DO - http://dx.doi.org/10.1016/j.surg.2015.06.028 PT - Journal Article LG - English <24. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26210225 AU - Wang W AU - Zheng C AU - Fang C AU - Li P AU - Xie J AU - Lin J AU - Zhan Y AU - Li W AU - Chen Y AU - Sun X AU - Xu D AU - Li Y AU - Huang C AU - Zhou Z FA - Wang, Wei FA - Zheng, Chaohui FA - Fang, Cheng FA - Li, Ping FA - Xie, Jianwei FA - Lin, Jianxian FA - Zhan, Youqing FA - Li, Wei FA - Chen, Yingbo FA - Sun, Xiaowei FA - Xu, Dazhi FA - Li, Yuanfang FA - Huang, Changming FA - Zhou, Zhiwei IN - Wang,Wei. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Zheng,Chaohui. Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, P.R. China. Fang,Cheng. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Li,Ping. Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, P.R. China. Xie,Jianwei. Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, P.R. China. Lin,Jianxian. Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, P.R. China. Zhan,Youqing. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Li,Wei. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Chen,Yingbo. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Sun,Xiaowei. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Xu,Dazhi. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Li,Yuanfang. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Huang,Changming. Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, P.R. China. Electronic address: hcmlr2002@163.com. Zhou,Zhiwei. Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P.R. China. Electronic address: zhouzhw@sysucc.org.cn. TI - Time trends of clinicopathologic features and surgical treatment for gastric cancer: Results from 2 high-volume institutions in southern China. SO - Surgery. 158(6):1590-7, 2015 Dec. AS - Surgery. 158(6):1590-7, 2015 Dec. NJ - Surgery LM - Available online from MWHC library: 1995 - present, Available in print through MWHC library: 1999 - 2006 AB - BACKGROUND: The objective of this study was to evaluate the time-related trends of tumor characteristics and postoperative survival of patients with gastric cancer (GC) in 2 high-volume centers in high incidence areas of southern China. AB - METHODS: Based on the meticulously collected data from 5,327 patients with GC treated with operative intervention at Sun Yat-sen University Cancer Center and Fujian Medical University Union Hospital, we analyzed the differences in clinicopathologic features and postoperative survival over the following 4 consecutive periods: 1991-1995 (period 1), 1996-2000 (period 2), 2001-2005 (period 3), and 2006-2010 (period 4). AB - RESULTS: Tumor size decreased (P = .001), but the proportion of poorly differentiated tumors increased (P < .001) over the study periods. Early GC was diagnosed more often in later periods, gradually increasing from 7 to 15% (P < .001). A surprising improvement was observed in the mean number of retrieved lymph nodes, ranging from 10.36 to 26.22 (P < .001). The radical resection rate increased from 88 to 93%. The overall 5-year survival rate improved steadily over the 4 periods, from 39 to 53% (P < .001). Multivariate analysis revealed that age, tumor location, histologic type, tumor size, depth of invasion, lymphatic invasion, number of retrieved lymph nodes, radical resection, and time periods were independent prognostic factors. AB - CONCLUSION: The clinicopathologic features of tumors changed during the observation period in our region. The increasingly early detection of patients with GC and more standardized regimens for operative management, including routinely performed D2 lymphadenectomy, most likely resulted in the increase in overall survival.Copyright © 2015 Elsevier Inc. All rights reserved. IL - 0039-6060 DO - http://dx.doi.org/10.1016/j.surg.2015.04.038 PT - Journal Article PT - Multicenter Study PT - Research Support, Non-U.S. Gov't LG - English <25. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26438688 AU - Zhu M AU - Goetsch SC AU - Wang Z AU - Luo R AU - Hill JA AU - Schneider J AU - Morris SM Jr AU - Liu ZP FA - Zhu, Min FA - Goetsch, Sean C FA - Wang, Zhaoning FA - Luo, Robert FA - Hill, Joseph A FA - Schneider, Jay FA - Morris, Sidney M Jr FA - Liu, Zhi-Ping IN - Zhu,Min. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). Goetsch,Sean C. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). Wang,Zhaoning. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). Luo,Robert. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). Hill,Joseph A. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). Schneider,Jay. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). Morris,Sidney M Jr. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). Liu,Zhi-Ping. From the Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX (M.Z., S.C.G., Z.W., R.L., J.A.H., J.S., Z.-P.L.); and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA (S.M.M.). zhi-ping.Liu@utsouthwestern.edu. TI - FoxO4 promotes early inflammatory response upon myocardial infarction via endothelial Arg1. SO - Circulation Research. 117(11):967-77, 2015 Nov 6. AS - Circ Res. 117(11):967-77, 2015 Nov 6. NJ - Circulation research LM - Available online from MWHC library: 1953 - present AB - RATIONALE: Inflammation in post-myocardial infarction (MI) is necessary for myocyte repair and wound healing. Unfortunately, it is also a key component of subsequent heart failure pathology. Transcription factor forkhead box O4 (FoxO4) regulates a variety of biological processes, including inflammation. However, its role in MI remains unknown. AB - OBJECTIVE: To test the hypothesis that FoxO4 promotes early post-MI inflammation via endothelial arginase 1 (Arg1). AB - METHODS AND RESULTS: We induced MI in wild-type and FoxO4(-/-) mice. FoxO4(-/-) mice had a significantly higher post-MI survival, better cardiac function, and reduced infarct size. FoxO4(-/-) hearts had significantly fewer neutrophils, reduced expression of cytokines, and competitive nitric oxide synthase inhibitor Arg1. We generated conditional FoxO4 knockout mice with FoxO4 deleted in cardiac mycoytes or endothelial cells. FoxO4 endothelial cell-specific knockout mice showed significant post-MI improvement of cardiac function and reduction of neutrophil accumulation and cytokine expression, whereas FoxO4 cardiac mycoyte-specific knockout mice had no significant difference in cardiac function and post-MI inflammation from those of control littermates. FoxO4 binds the Foxo-binding site in the Arg1 promoter and activates Arg1 transcription. FoxO4 knockdown in human aortic endothelial cells upregulated nitric oxide on ischemia and suppressed monocyte adhesion that can be reversed by ectopic-expression of Arg1. Furthermore, chemical inhibition of Arg1 in wild-type mice had similar cardioprotection and reduced inflammation after MI as FoxO4 inactivation and administration of nitric oxide synthase inhibitor to FoxO4 KO mice reversed the beneficial effects of FoxO4 deletion on post-MI cardiac function. AB - CONCLUSIONS: FoxO4 activates Arg1 transcription in endothelial cells in response to MI, leading to downregulation of nitric oxide and upregulation of neutrophil infiltration to the infarct area.Copyright © 2015 American Heart Association, Inc. IL - 0009-7330 DO - http://dx.doi.org/10.1161/CIRCRESAHA.115.306919 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English <26. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26436306 AU - Wang C AU - Zhao P AU - Luo P AU - Liu W AU - Wang H AU - Zhao Q FA - Wang, Chunya FA - Zhao, Pan FA - Luo, Ping FA - Liu, Weiwei FA - Wang, Hao FA - Zhao, Quanming IN - Wang,Chunya. a 1 Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University , Beijing 100029, China. Zhao,Pan. b 2 Clinical Trial Center, Beijing 302 Hospital (302 Hospital of PLA) , Beijing 100039, China. Luo,Ping. a 1 Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University , Beijing 100029, China. Liu,Weiwei. c 3 Department of Statistics, Academy of Military Medical Science , Beijing 100850, China. Wang,Hao. d 4 Medical Record Center, Beijing 302 Hospital (302 Hospital of PLA) , Beijing 100039, China. Zhao,Quanming. e 5 Department of Special Medical Service, Beijing Anzhen Hospital, Capital Medical University , Beijing 100029, China. TI - Prevalence and risk factors of coronary artery disease in patients with chronic viral hepatitis. SO - Postgraduate Medicine. 127(8):786-90, 2015. AS - Postgrad Med. 127(8):786-90, 2015. NJ - Postgraduate medicine LM - Available in print through MWHC library: 1999 - 2006 AB - OBJECTIVES: Patients with chronic viral hepatitis usually present metabolic abnormalities and hemodynamic changes, which are known factors associated with the development of coronary artery disease (CAD). This study aims to determine the risk factors of incident CAD in these patients. AB - METHODS: We identified 193 patients who subsequently developed CAD amongst 37,840 cases diagnosed as chronic viral hepatitis from January 2007 through December 2013. AB - RESULTS: In these patients, 141 had hepatitis B virus infections and 52 had hepatitis C virus infections. There was a male preponderance (65.9%). The median age at the diagnosis of hepatitis was 51 years. In the univariate analysis, patients aged > 51 years had shorter median periods from the diagnosis of hepatitis to the onset of CAD than patients aged < 51 years (50 versus 96 months, p < 0.001), and patients with hypertension had shorter median durations compared to those without hypertension (48 versus 96 months, p < 0.001). Statistical significance also existed between patients with different etiologies (p = 0.004). In the multivariate analysis by Cox regression, age at the diagnosis of hepatitis (p < 0.001; hazard ratio (HR), 1.041; 95% CI, 1.027-1.056) and hypertension (with versus without, p < 0.001; HR, 1.925; 95% CI, 1.419-2.611) were revealed. AB - CONCLUSIONS: Age at diagnosis of hepatitis and hypertension appeared to be independent risk factors of incident CAD in these patients. This topic deserves further studies. IL - 0032-5481 DO - http://dx.doi.org/10.1080/00325481.2015.1094366 PT - Journal Article LG - English <27. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26395861 AU - Wang H AU - Fox CS AU - Troy LM AU - Mckeown NM AU - Jacques PF FA - Wang, Huifen FA - Fox, Caroline S FA - Troy, Lisa M FA - Mckeown, Nicola M FA - Jacques, Paul F IN - Wang,Huifen. 1Jean Mayer US Department of Agriculture (USDA) Human Nutrition Research Center on Aging,Tufts University,711 Washington St,Boston,MA 02111,USA. Fox,Caroline S. 2National Heart, Lung, and Blood Institute's Framingham Heart Study,Framingham,MA 01702,USA. Troy,Lisa M. 3Department of Nutrition,Chenoweth Laboratory,University of Massachusetts,Amherst,MA 01003,USA. Mckeown,Nicola M. 1Jean Mayer US Department of Agriculture (USDA) Human Nutrition Research Center on Aging,Tufts University,711 Washington St,Boston,MA 02111,USA. Jacques,Paul F. 1Jean Mayer US Department of Agriculture (USDA) Human Nutrition Research Center on Aging,Tufts University,711 Washington St,Boston,MA 02111,USA. TI - Longitudinal association of dairy consumption with the changes in blood pressure and the risk of incident hypertension: the Framingham Heart Study. SO - British Journal of Nutrition. 114(11):1887-99, 2015 Dec 14. AS - Br J Nutr. 114(11):1887-99, 2015 Dec 14. NJ - The British journal of nutrition AB - We aimed to examine the longitudinal association of dairy consumption with the changes in blood pressure (BP) and the risk of incident hypertension (HTN) among adults. This study included 2636 Framingham Heart Study Offspring Cohort members who participated in the 5th through 8th examinations (1991-2008) and were free of HTN at their first examination during the follow-up. Data collected at each examination included dietary intake (by a validated FFQ), BP (following standardised procedures) and anti-hypertensive medication use (by physician-elicited self-report). HTN was defined as systolic BP (SBP)>140 mmHg, or diastolic BP (DBP)>90 mmHg or anti-hypertensive medication use. We used repeated-measure and discrete-time hazard regressions to examine the associations of dairy consumption with the annualised BP change (n 2075) and incident HTN (n 2340; cases=1026), respectively. Covariates included demographic, lifestyle, overall diet quality, metabolic factors and medication use. Greater intakes of total dairy foods, total low-fat/fat-free dairy foods, low-fat/skimmed milk and yoghurt were associated with smaller annualised increments in SBP and a lower risk of projected HTN incidence. However, with the exception of total dairy foods and yoghurt, these inverse associations with HTN risk were attenuated as the follow-up time increased. For yoghurt, each additional serving was associated with 6 (95 % CI 1, 10) % reduced risk of incident HTN. Total dairy and total low-fat/fat-free dairy intakes were found to be inversely related to changes in DBP. Dairy consumption, as part of a nutritious and energy-balanced diet pattern, may benefit BP control and prevent or delay the onset of HTN. IL - 0007-1145 DO - http://dx.doi.org/10.1017/S0007114515003578 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. LG - English <28. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26135024 AU - Crotty Alexander LE AU - Shin S AU - Hwang JH FA - Crotty Alexander, Laura E FA - Shin, Stephanie FA - Hwang, John H TI - Inflammatory Diseases of the Lung Induced by Conventional Cigarette Smoke: A Review. [Review] SO - Chest. 148(5):1307-22, 2015 Nov. AS - Chest. 148(5):1307-22, 2015 Nov. NJ - Chest LM - Available online from MWHC library: 1935 - present, Available in print through MWHC library: 1999 - 2006 AB - Smoking-induced lung diseases were extremely rare prior to the 20th century. With commercialization and introduction of machine-made cigarettes, worldwide use skyrocketed and several new pulmonary diseases have been recognized. The majority of pulmonary diseases caused by cigarette smoke (CS) are inflammatory in origin. Airway epithelial cells and alveolar macrophages have altered inflammatory signaling in response to CS, which leads to recruitment of lymphocytes, eosinophils, neutrophils, and mast cells to the lungs-depending on the signaling pathway (nuclear factor-kappaB, adenosine monophosphate-activated protein kinase, c-Jun N-terminal kinase, p38, and signal transducer and activator of transcription 3) activated. Multiple proteins are upregulated and secreted in response to CS exposure, and many of these have immunomodulatory activities that contribute to disease pathogenesis. In particular, metalloproteases 9 and 12, surfactant protein D, antimicrobial peptides (LL-37 and human beta defensin 2), and IL-1, IL-6, IL-8, and IL-17 have been found in higher quantities in the lungs of smokers with ongoing inflammation. However, many underlying mechanisms of smoking-induced inflammatory diseases are not yet known. We review here the known cellular and molecular mechanisms of CS-induced diseases, including COPD, respiratory bronchiolitis-interstitial lung disease, desquamative interstitial pneumonia, acute eosinophilic pneumonia, chronic rhinosinusitis, pulmonary Langerhans cell histiocytosis, and chronic bacterial infections. We also discuss inflammation induced by secondhand and thirdhand smoke exposure and the pulmonary diseases that result. New targeted antiinflammatory therapeutic options are currently under investigation and hopefully will yield promising results for the treatment of these highly prevalent smoking-induced diseases. IL - 0012-3692 DO - http://dx.doi.org/10.1378/chest.15-0409 PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Review LG - English <29. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26134718 AU - Lee KK AU - Ward K AU - Rafferty GF AU - Moxham J AU - Birring SS FA - Lee, Kai K FA - Ward, Katie FA - Rafferty, Gerrard F FA - Moxham, John FA - Birring, Surinder S TI - The Intensity of Voluntary, Induced, and Spontaneous Cough. SO - Chest. 148(5):1259-67, 2015 Nov. AS - Chest. 148(5):1259-67, 2015 Nov. NJ - Chest LM - Available online from MWHC library: 1935 - present, Available in print through MWHC library: 1999 - 2006 AB - BACKGROUND: The intensity of cough is an important determinant of cough severity. Few studies have quantified cough intensity in patients with chronic cough with objective measures. We investigated the intensity of voluntary, induced, and spontaneous cough in patients with chronic cough and healthy control subjects. AB - METHODS: Patients with chronic cough and control subjects underwent physiologic assessment of the intensity of maximum voluntary, capsaicin-induced, and spontaneous cough. Assessments included measurement of gastric pressure (Pga) and esophageal pressure (Pes) during cough, peak cough flow (PCF), expiratory muscle strength (twitch gastric pressure [TwPga]), and cough compression phase duration (CPD). Subjective perception of cough intensity was assessed using a visual analog scale (VAS). AB - RESULTS: Pes, Pga, and PCF during maximum voluntary cough were significantly greater in patients with chronic cough compared with control subjects (P = .003-.042). There was no difference in TwPga between patients and control subjects. CPD was increased in female patients compared with control subjects (mean +/- SD, 0.50 +/- 0.22 s vs 0.28 +/- 0.17 s; P = .007). Mean +/- SD Pes during spontaneous cough was comparable to induced cough (128 +/- 28 cm H2O vs 122 +/- 37 cm H2O, P = .686) but less than maximum voluntary cough (170 +/- 46 cm H2O, P = .020). Median within-subject correlation coefficients between cough intensity VAS and Pes, Pga, and PCF were r = 0.82 to 0.86. AB - CONCLUSIONS: Maximum voluntary cough intensity was increased in patients with chronic cough compared with control subjects. There was no significant difference in expiratory muscle contractility. Further studies should evaluate the compressive phase of cough in more detail. Physiologic measures of cough intensity correlated strongly with subjective perception of intensity in patients with chronic cough and may be relevant objective outcome measures for clinical studies. IL - 0012-3692 DO - http://dx.doi.org/10.1378/chest.15-0138 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <30. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26065915 AU - Guarnieri M AU - Diaz E AU - Pope D AU - Eisen EA AU - Mann J AU - Smith KR AU - Smith-Sivertsen T AU - Bruce NG AU - Balmes JR FA - Guarnieri, Michael FA - Diaz, Esperanza FA - Pope, Daniel FA - Eisen, Ellen A FA - Mann, Jennifer FA - Smith, Kirk R FA - Smith-Sivertsen, Tone FA - Bruce, Nigel G FA - Balmes, John R TI - Lung Function in Rural Guatemalan Women Before and After a Chimney Stove Intervention to Reduce Wood Smoke Exposure: Results From the Randomized Exposure Study of Pollution Indoors and Respiratory Effects and Chronic Respiratory Effects of Early Childhood Exposure to Respirable Particulate Matter Study. SO - Chest. 148(5):1184-92, 2015 Nov. AS - Chest. 148(5):1184-92, 2015 Nov. NJ - Chest LM - Available online from MWHC library: 1935 - present, Available in print through MWHC library: 1999 - 2006 AB - BACKGROUND: COPD is the third most frequent cause of death globally, with much of this burden attributable to household biomass smoke exposure in developing countries. As biomass smoke exposure is also associated with cardiovascular disease, lower respiratory infection, lung cancer, and cataracts, it presents an important target for public health intervention. AB - METHODS: Lung function in Guatemalan women exposed to wood smoke from open fires was measured throughout the Randomized Exposure Study of Pollution Indoors and Respiratory Effects (RESPIRE) stove intervention trial and continued during the Chronic Respiratory Effects of Early Childhood Exposure to Respirable Particulate Matter (CRECER) cohort study. In RESPIRE, early stove households received a chimney woodstove at the beginning of the 18-month trial, and delayed stove households received a stove at trial completion. Personal exposure to wood smoke was assessed with exhaled breath carbon monoxide (CO) and personal CO tubes. Change in lung function between intervention groups and as a function of wood smoke exposure was assessed using random effects models. AB - RESULTS: Of 306 women participating in both studies, acceptable spirometry was collected in 129 early stove and 136 delayed stove households (n = 265), with a mean follow-up of 5.6 years. Despite reduced wood smoke exposures in early stove households, there were no significant differences in any of the measured spirometric variables during the study period (FEV1, FVC, FEV1/FVC ratio, and annual change) after adjustment for confounding. AB - CONCLUSIONS: In these young Guatemalan women, there was no association between lung function and early randomization to a chimney stove or personal wood smoke exposure. Future stove intervention trials should incorporate cleaner stoves, longer follow-up, or potentially susceptible groups to identify meaningful differences in lung function. IL - 0012-3692 DO - http://dx.doi.org/10.1378/chest.15-0261 PT - Journal Article PT - Randomized Controlled Trial PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English <31. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25763792 AU - Ng SS AU - Chan RS AU - Woo J AU - Chan TO AU - Cheung BH AU - Sea MM AU - To KW AU - Chan KK AU - Ngai J AU - Yip WH AU - Ko FW AU - Hui DS FA - Ng, Susanna S S FA - Chan, Ruth S M FA - Woo, Jean FA - Chan, Tat-On FA - Cheung, Bernice H K FA - Sea, Mandy M M FA - To, Kin-Wang FA - Chan, Ken K P FA - Ngai, Jenny FA - Yip, Wing-Ho FA - Ko, Fanny W S FA - Hui, David S C TI - A Randomized Controlled Study to Examine the Effect of a Lifestyle Modification Program in OSA. SO - Chest. 148(5):1193-203, 2015 Nov. AS - Chest. 148(5):1193-203, 2015 Nov. NJ - Chest LM - Available online from MWHC library: 1935 - present, Available in print through MWHC library: 1999 - 2006 AB - BACKGROUND: Obesity is an important risk factor for OSA. This study aimed to assess the effect of weight reduction through a lifestyle modification program (LMP) on patients with moderate to severe OSA. AB - METHODS: This was a parallel group, randomized controlled trial. Altogether, 104 patients with moderate to severe OSA diagnosed on portable home sleep monitoring were randomized to receive a dietician-led LMP or usual care for 12 months. The primary outcome was reduction of apnea-hypopnea index (AHI) at 12 months as assessed by portable home sleep monitoring. AB - RESULTS: In the intention-to-treat analysis (ITT), LMP (n = 61) was more effective in reducing AHI from baseline (16.9% fewer events in the LMP group vs 0.6% more events in the control group, P = .011). LMP was more effective in reducing BMI (-1.8 kg/m2, 6.0% of the initial BMI; -0.6 kg/m2, 2.0% of the initial BMI in control group; P < .001). The reduction in daytime sleepiness as assessed by Epworth Sleepiness Scale was not significant in ITT but was more in the LMP group (-3.5 in the LMP group vs -1.1 in the control group, P = .004) by treatment per protocol analysis. There was modest improvement in mental health in the Short Form Health Survey. Eating behavior was improved with increased intake of protein and fiber. These changes were observed 4 months after the initial intensive diet counseling and persisted at 12 months. AB - CONCLUSIONS: LMP was effective in reducing the severity of OSA and daytime sleepiness. The beneficial effect was sustained in 12 months. AB - TRIAL REGISTRY: ClinicalTrials.gov; No.: NCT01384760; URL: www.clinicaltrials.gov. IL - 0012-3692 DO - http://dx.doi.org/10.1378/chest.14-3016 PT - Journal Article PT - Randomized Controlled Trial PT - Research Support, Non-U.S. Gov't LG - English <32. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26389572 AU - Slomovic S AU - Collins JJ FA - Slomovic, Shimyn FA - Collins, James J IN - Slomovic,Shimyn. Institute for Medical Engineering &Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA. Slomovic,Shimyn. Department of Biological Engineering, MIT, Cambridge, Massachusetts, USA. Slomovic,Shimyn. Synthetic Biology Center, MIT, Cambridge, Massachusetts, USA. Collins,James J. Institute for Medical Engineering &Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA. Collins,James J. Department of Biological Engineering, MIT, Cambridge, Massachusetts, USA. Collins,James J. Synthetic Biology Center, MIT, Cambridge, Massachusetts, USA. Collins,James J. Harvard-MIT Program in Health Sciences and Technology, Cambridge, Massachusetts, USA. Collins,James J. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. Collins,James J. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA. TI - DNA sense-and-respond protein modules for mammalian cells. SO - Nature Methods. 12(11):1085-90, 2015 Nov. AS - Nat Methods. 12(11):1085-90, 2015 Nov. NJ - Nature methods AB - We generated synthetic protein components that can detect specific DNA sequences and subsequently trigger a desired intracellular response. These modular sensors exploit the programmability of zinc-finger DNA recognition to drive the intein-mediated splicing of an artificial trans-activator that signals to a genetic circuit containing a given reporter or response gene. We used the sensors to mediate sequence recognition-induced apoptosis as well as to detect and report a viral infection. This work establishes a synthetic biology framework for endowing mammalian cells with sentinel capabilities, which provides a programmable means to cull infected cells. It may also be used to identify positively transduced or transfected cells, isolate recipients of intentional genomic edits and increase the repertoire of inducible parts in synthetic biology. IL - 1548-7091 DO - http://dx.doi.org/10.1038/nmeth.3585 PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. LG - English <33. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26344044 AU - Kiani S AU - Chavez A AU - Tuttle M AU - Hall RN AU - Chari R AU - Ter-Ovanesyan D AU - Qian J AU - Pruitt BW AU - Beal J AU - Vora S AU - Buchthal J AU - Kowal EJ AU - Ebrahimkhani MR AU - Collins JJ AU - Weiss R AU - Church G AI - Beal, Jacob; ORCID: http://orcid.org/0000-0002-1663-5102, Vora, Suhani; ORCID: http://orcid.org/0000-0003-4618-2017 FA - Kiani, Samira FA - Chavez, Alejandro FA - Tuttle, Marcelle FA - Hall, Richard N FA - Chari, Raj FA - Ter-Ovanesyan, Dmitry FA - Qian, Jason FA - Pruitt, Benjamin W FA - Beal, Jacob FA - Vora, Suhani FA - Buchthal, Joanna FA - Kowal, Emma J K FA - Ebrahimkhani, Mohammad R FA - Collins, James J FA - Weiss, Ron FA - Church, George IN - Kiani,Samira. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Kiani,Samira. Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Chavez,Alejandro. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Chavez,Alejandro. Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts, USA. Chavez,Alejandro. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. Tuttle,Marcelle. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Hall,Richard N. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Hall,Richard N. Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Chari,Raj. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. Ter-Ovanesyan,Dmitry. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Ter-Ovanesyan,Dmitry. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. Qian,Jason. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Qian,Jason. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. Pruitt,Benjamin W. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Beal,Jacob. Raytheon BBN Technologies, Cambridge, Massachusetts, USA. Vora,Suhani. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Vora,Suhani. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Vora,Suhani. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. Buchthal,Joanna. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Kowal,Emma J K. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Ebrahimkhani,Mohammad R. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Ebrahimkhani,Mohammad R. Center for Emergent Behaviors of Integrated Cellular Systems (EBICS), Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Collins,James J. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Collins,James J. Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Collins,James J. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Collins,James J. Institute for Medical Engineering &Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Collins,James J. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. Collins,James J. Harvard-MIT Program in Health Sciences and Technology, Cambridge, Massachusetts, USA. Weiss,Ron. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Weiss,Ron. Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Weiss,Ron. Center for Emergent Behaviors of Integrated Cellular Systems (EBICS), Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Church,George. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. Church,George. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. TI - Cas9 gRNA engineering for genome editing, activation and repression. SO - Nature Methods. 12(11):1051-4, 2015 Nov. AS - Nat Methods. 12(11):1051-4, 2015 Nov. NJ - Nature methods AB - We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein. IL - 1548-7091 DO - http://dx.doi.org/10.1038/nmeth.3580 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. LG - English <34. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26367177 AU - Li HW AU - Meng Y AU - Xie Q AU - Yi WJ AU - Lai XL AU - Bian Q AU - Wang J AU - Wang JF AU - Yu G FA - Li, He-wen FA - Meng, Yan FA - Xie, Qun FA - Yi, Wen-jing FA - Lai, Xue-li FA - Bian, Qi FA - Wang, Jun FA - Wang, Jia-feng FA - Yu, Guang IN - Li,He-wen. Department of Nephrology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Meng,Yan. Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Xie,Qun. Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Yi,Wen-jing. Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Lai,Xue-li. Department of Nephrology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Bian,Qi. Department of Nephrology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Wang,Jun. Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Wang,Jia-feng. Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Electronic address: jfwang@smmu.edu.cn. Yu,Guang. Department of Nephrology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Electronic address: yug88808@126.com. TI - miR-98 protects endothelial cells against hypoxia/reoxygenation induced-apoptosis by targeting caspase-3. SO - Biochemical & Biophysical Research Communications. 467(3):595-601, 2015 Nov 20. AS - Biochem Biophys Res Commun. 467(3):595-601, 2015 Nov 20. NJ - Biochemical and biophysical research communications AB - Endothelial dysfunction is one of the main pathophysiological processes involved in renal ischemia reperfusion injury. Our previous microarray study demonstrated that miR-98 was upregulated in the kidney with ischemia reperfusion injury (IRI). The present study was performed to investigate whether miR-98 was involved in the regulation of endothelial apoptosis under hypoxia and re-oxygenation (H/R) conditions. The dynamic changes of miR-98 in mouse IRI kidney and H/R HUVECs was measured. HUVECs were treated with HIF-1alpha siRNA to investigate the role of HIF-1alpha on miR-98 expression. The potential target genes of miR-98 were predicted by bioinformatics analyses. HUVECs were transfected with miR-98 mimics or inhibitor to confirm the role of miR-98 on the expression of target genes and hypoxia-induced apoptosis. The target gene was finally confirmed by dual-luciferase reporter assay. Both of IRI and H/R induced significantly up-regulation of miR-98 in the ischemic kidney and hypoxic HUVECs. HIF-1alpha siRNA remarkably down-regulated the expression of miR-98 in both normal and hypoxic HUVECs. The putative target genes of miR-98 included IL-6, IL-10 and caspase-3. MiR-98 mimics significantly inhibit caspase-3 expression in HUVECs, while anti-miR-98 significantly up-regulated it. But no change of IL-6 and IL-10 levels was observed after miRNA transfection. miR-98 protected HUVECs against apoptosis induced by hypoxia, while anti-miR-98 had the reverse effect. Furthermore, the dual-luciferase reporter assay confirmed that miR-98 decreased the luciferase activity by targeting the 3' untranslated region of caspase-3. In conclusion, Renal IRI induces up-regulation of miR-98 dependent on HIF-1alpha, which protects endothelial cells against apoptosis by targeting caspase-3. Copyright © 2015 Elsevier Inc. All rights reserved. IL - 0006-291X DO - http://dx.doi.org/10.1016/j.bbrc.2015.09.058 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <35. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26390336 AU - Nichols DP AU - Kuk KN AU - Nick JA FA - Nichols, David P FA - Kuk, Kelly N FA - Nick, Jerry A IN - Nichols,David P. aDepartment of Pediatrics bDepartment of Medicine, National Jewish Health cDepartment of Pharmacy, St. Joseph Hospital SCL Health, Denver dDepartment of Medicine eDepartment of Pediatrics, University of Colorado Denver School of Medicine, Aurora, Colorado, USA. TI - Drug interactions and treatment burden as survival improves. [Review] SO - Current Opinion in Pulmonary Medicine. 21(6):617-25, 2015 Nov. AS - Curr Opin Pulm Med. 21(6):617-25, 2015 Nov. NJ - Current opinion in pulmonary medicine LM - Available online from MWHC library: 1999 - present AB - PURPOSE OF REVIEW: With our growing understanding of the pathophysiology of cystic fibrosis, the pace of drug discovery is accelerating. Newer agents and therapies have traditionally been added to available medications, given the urgency in treating the disease. As the cystic fibrosis population ages, the number of associated comorbidities increases, requiring additional therapeutic approaches. Thus, while current management strategies have dramatically extended projected life expectancy, the treatment burden of the disease in adulthood has become onerous, and there is increasing concern over unintended effects and drug-drug interactions of new and existing therapies. AB - RECENT FINDINGS: A number of recent studies have sought to quantify the treatment burden of cystic fibrosis care, and to identify ways to reduce this burden. Mechanistic studies have identified the potential for a number of cystic fibrosis medications to impair the host response, or to interfere with the efficacy of other agents. AB - SUMMARY: As the cystic fibrosis formulary grows, a primary emphasis will be for providers to develop personalized treatment plans, with a goal to reduce unnecessary treatment burden and an awareness of potential unanticipated effects of medications. IL - 1070-5287 DO - http://dx.doi.org/10.1097/MCP.0000000000000212 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Review LG - English <36. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26238710 AU - Pietrocola G AU - Gianotti V AU - Richards A AU - Nobile G AU - Geoghegan JA AU - Rindi S AU - Monk IR AU - Bordt AS AU - Foster TJ AU - Fitzgerald JR AU - Speziale P FA - Pietrocola, Giampiero FA - Gianotti, Valentina FA - Richards, Amy FA - Nobile, Giulia FA - Geoghegan, Joan A FA - Rindi, Simonetta FA - Monk, Ian R FA - Bordt, Andrea S FA - Foster, Timothy J FA - Fitzgerald, J Ross FA - Speziale, Pietro IN - Pietrocola,Giampiero. Department of Molecular Medicine, Unit of Biochemistry, Pavia, Italy. Gianotti,Valentina. Department of Molecular Medicine, Unit of Biochemistry, Pavia, Italy. Richards,Amy. The Roslin Institute and Edinburgh Infectious Diseases, University of Edinburgh, Easter Bush, Midlothian, Scotland, United Kingdom. Nobile,Giulia. Department of Molecular Medicine, Unit of Biochemistry, Pavia, Italy. Geoghegan,Joan A. Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin, Ireland. Rindi,Simonetta. Department of Molecular Medicine, Unit of Biochemistry, Pavia, Italy. Monk,Ian R. Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin, Ireland. Bordt,Andrea S. Center for Infectious and Inflammatory Diseases, Texas A&M Health Science Center, Houston, Texas, USA. Foster,Timothy J. Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin, Ireland. Fitzgerald,J Ross. The Roslin Institute and Edinburgh Infectious Diseases, University of Edinburgh, Easter Bush, Midlothian, Scotland, United Kingdom. Speziale,Pietro. Department of Molecular Medicine, Unit of Biochemistry, Pavia, Italy pspeziale@unipv.it. TI - Fibronectin Binding Proteins SpsD and SpsL Both Support Invasion of Canine Epithelial Cells by Staphylococcus pseudintermedius. SO - Infection & Immunity. 83(10):4093-102, 2015 Oct. AS - Infect Immun. 83(10):4093-102, 2015 Oct. NJ - Infection and immunity LM - Available online from MWHC library: 1970 - present (after 4 mo) AB - In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (CPEK). Invasion was examined by the gentamicin protection assay and fluorescence microscopy. An DELTAspsD DELTAspsL mutant of strain ED99 had a dramatically reduced capacity to invade CPEK monolayers, while no difference in the invasion level was observed with single mutants. Lactococcus lactis transformed with plasmids expressing SpsD and SpsL promoted invasion, showing that both proteins are important. Soluble fibronectin was required for invasion, and an RGD-containing peptide or antibodies recognizing the integrin alpha5beta1 markedly reduced invasion, suggesting an important role for the integrin in this process. Src kinase inhibitors effectively blocked internalization, suggesting a functional role for the kinase in invasion. In order to identify the minimal fibronectin-binding region of SpsD and SpsL involved in the internalization process, recombinant fragments of both proteins were produced. The SpsD520-846 and SpsL538-823 regions harboring the major fibronectin-binding sites inhibited S. pseudintermedius internalization. Finally, the effects of staphylococcal invasion on the integrity of different cell lines were examined. Because SpsD and SpsL are critical factors for adhesion and invasion, blocking these processes could provide a strategy for future approaches to treating infections. Copyright © 2015, American Society for Microbiology. All Rights Reserved. IL - 0019-9567 DO - http://dx.doi.org/10.1128/IAI.00542-15 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <37. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26195552 AU - Sheng Y AU - Fan F AU - Jensen O AU - Zhong Z AU - Kan B AU - Wang H AU - Zhu J FA - Sheng, Ying FA - Fan, Fenxia FA - Jensen, Owen FA - Zhong, Zengtao FA - Kan, Biao FA - Wang, Hui FA - Zhu, Jun IN - Sheng,Ying. Department of Microbiology, Nanjing Agricultural University, Nanjing, China State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Fan,Fenxia. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Jensen,Owen. Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA. Zhong,Zengtao. Department of Microbiology, Nanjing Agricultural University, Nanjing, China. Kan,Biao. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China. Wang,Hui. Department of Microbiology, Nanjing Agricultural University, Nanjing, China wanghui@njau.edu.cn junzhu@mail.med.upenn.edu. Zhu,Jun. Department of Microbiology, Nanjing Agricultural University, Nanjing, China Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA wanghui@njau.edu.cn junzhu@mail.med.upenn.edu. TI - Dual Zinc Transporter Systems in Vibrio cholerae Promote Competitive Advantages over Gut Microbiome. SO - Infection & Immunity. 83(10):3902-8, 2015 Oct. AS - Infect Immun. 83(10):3902-8, 2015 Oct. NJ - Infection and immunity LM - Available online from MWHC library: 1970 - present (after 4 mo) AB - Zinc is an essential trace metal required for numerous cellular processes in all forms of life. In order to maintain zinc homeostasis, bacteria have developed several transport systems to regulate its uptake. In this study, we investigated zinc transport systems in the enteric pathogen Vibrio cholerae, the causative agent of cholera. Bioinformatic analysis predicts that two gene clusters, VC2081 to VC2083 (annotated as zinc utilization genes znuABC) and VC2551 to VC2555 (annotated as zinc-regulated genes zrgABCDE), are regulated by the putative zinc uptake regulator Zur. Using promoter reporter and biochemical assays, we confirmed that Zur represses znuABC and zrgABCDE promoters in a Zn(2+)-dependent manner. Under Zn(2+)-limiting conditions, we found that mutations in either the znuABC or zrgABCDE gene cluster affect bacterial growth, with znuABC mutants displaying a more severe growth defect, suggesting that both ZnuABC and ZrgABCDE are involved in Zn(2+) uptake and that ZnuABC plays the predominant role. Furthermore, we reveal that ZnuABC and ZrgABCDE are important for V. cholerae colonization in both infant and adult mouse models, particularly in the presence of other intestinal microbiota. Collectively, our studies indicate that these two zinc transporter systems play vital roles in maintaining zinc homeostasis during V. cholerae growth and pathogenesis. Copyright © 2015, American Society for Microbiology. All Rights Reserved. IL - 0019-9567 DO - http://dx.doi.org/10.1128/IAI.00447-15 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English <38. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26257073 AU - Zhu J AU - Chen F AU - Wang L AU - Niu Y AU - Yu D AU - Shu C AU - Chen H AU - Wang H AU - Xiao Z FA - Zhu, JianCai FA - Chen, Feng FA - Wang, LingYing FA - Niu, YunWei FA - Yu, Dan FA - Shu, Chang FA - Chen, HeXing FA - Wang, HongLin FA - Xiao, ZuoBing IN - Zhu,JianCai. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. Chen,Feng. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. Chen,Feng. Department of Food, Nutrition, and Packaging Sciences, Clemson University , Clemson, South Carolina 29634, United States. Wang,LingYing. Shanghai Cosmax (China) Cosmetics Co., LTD , Shanghai, China. Niu,YunWei. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. Yu,Dan. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. Shu,Chang. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. Chen,HeXing. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. Wang,HongLin. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. Xiao,ZuoBing. Department of Perfume and Aroma Technology, Shanghai Institute of Technology , Shanghai, 201418, China. TI - Comparison of Aroma-Active Volatiles in Oolong Tea Infusions Using GC-Olfactometry, GC-FPD, and GC-MS. SO - Journal of Agricultural & Food Chemistry. 63(34):7499-510, 2015 Sep 2. AS - J Agric Food Chem. 63(34):7499-510, 2015 Sep 2. NJ - Journal of agricultural and food chemistry AB - The aroma profile of oolong tea infusions (Dongdingwulong, DDWL; Tieguanyin, TGY; Dahongpao, DHP) were investigated in this study. Gas chromatography-olfactometry (GC-O) with the method of aroma intensity (AI) was employed to investigate the aroma-active compounds in tea infusions. The results presented forty-three, forty-five, and forty-eight aroma-active compounds in the TGY, DHP, and DDWL infusions, including six, seven, and five sulfur compounds, respectively. In addition, the concentration of volatile compounds in the tea infusions was further quantitated by solid phase microextraction-gas chromatography (SPME)-GC-MS and SPME-GC-flame photometric detection (FPD). Totally, seventy-six and thirteen volatile and sulfur compounds were detected in three types of tea infusions, respectively. Quantitative results showed that forty-seven aroma compounds were at concentrations higher than their corresponding odor thresholds. On the basis of the odor activity values (OAVs), 2-methylpropanal (OAV: 230-455), 3-methylbutanal (1-353), 2-methylbutanal (34-68), nerolidol (108-184), (E)-2-heptenal (148-294), hexanal (134-230), octanal (28-131), beta-damascenone (29-59), indole (96-138), 6-methyl-5-hepten-2-one (34-67), (R)-(-)-linalool (63-87), and dimethyl sulfide (7-1320) presented relatively higher OAVs than those of other compounds, indicating the importance of these compounds in the overall aroma of tea infusions. IL - 0021-8561 DO - http://dx.doi.org/10.1021/acs.jafc.5b02358 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <39. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26208876 AU - Liu A AU - Yu W AU - Liu M AU - Bai J AU - Liu W AU - Liu X AU - Pei H AU - Hu L AU - Huang M AU - Wang X FA - Liu, Aiping FA - Yu, Wanqi FA - Liu, Minhua FA - Bai, Jianjun FA - Liu, Weidong FA - Liu, Xingping FA - Pei, Hui FA - Hu, Li FA - Huang, Mingzhi FA - Wang, Xiaoguang IN - Liu,Aiping. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Liu,Aiping. Hunan Province Key Laboratory for Agrochemicals , Changsha 410014, People's Republic of China. Yu,Wanqi. Department of Chemistry, Carleton College , Northfield, Minnesota 55057, United States. Liu,Minhua. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Bai,Jianjun. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Liu,Weidong. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Liu,Xingping. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Pei,Hui. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Hu,Li. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Huang,Mingzhi. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. Huang,Mingzhi. Hunan Haili Chemical Industry Company Ltd., Changsha 410007, People's Republic of China. Wang,Xiaoguang. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry , Changsha 410007, People's Republic of China. TI - Synthesis and Insecticidal Activity of Novel Nitropyridyl-Based Dichloropropene Ethers. SO - Journal of Agricultural & Food Chemistry. 63(34):7469-75, 2015 Sep 2. AS - J Agric Food Chem. 63(34):7469-75, 2015 Sep 2. NJ - Journal of agricultural and food chemistry AB - Dihalopropene ether insecticides are known for good features such as no cross-resistance to other insecticide classes and safety for mammals. Pyridalyl is the only currently commercialized dichloropropene ether insecticide; however, it contains a trifluoromethyl group, the synthesis of which requires harsh reagents and reaction conditions. To search for novel dihalopropene ethers with unique biological activities but without trifluoromethyl groups, a series of nitropyridyl-based dichloropropene ether analogues were synthesized by reacting nitro-based halopyridine with 2,6-dichloro-4-(3,3-dichloroallyloxy)phenol or 2,6-dichloro-4-(3,3-dichloroallyloxy)phenyl 3-hydroxypropyl ether. Bioassay showed that the compounds exhibited potent insecticidal activities against various lepidopteran pests. Particularly, 2,6-dichloro-4-(3,3-dichloroallyloxy)phenyl 3-(5-nitro-2-pyridyloxy)propyl ether (8e) was active against major agricultural pests, and its insecticidal potency was comparable to that of Pyridalyl. Besides the trifluoromethyl group in Pyridalyl, a nitro group on the 5-position of the pyridyl ring is also viable for the development of optimal insecticidal activity. IL - 0021-8561 DO - http://dx.doi.org/10.1021/acs.jafc.5b02279 PT - Letter PT - Research Support, Non-U.S. Gov't LG - English <40. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26146887 AU - Hill PL AU - Turiano NA AU - Spiro A 3rd AU - Mroczek DK FA - Hill, Patrick L FA - Turiano, Nicholas A FA - Spiro, Avron 3rd FA - Mroczek, Daniel K IN - Hill,Patrick L. Department of Psychology, Carleton University. Turiano,Nicholas A. Department of Psychology, West Virginia University. Spiro,Avron 3rd. Department of Veterans Affairs Boston Healthcare System. Mroczek,Daniel K. Department of Psychology, Northwestern University. TI - Understanding inter-individual variability in purpose: Longitudinal findings from the VA Normative Aging Study. SO - Psychology & Aging. 30(3):529-33, 2015 Sep. AS - Psychol Aging. 30(3):529-33, 2015 Sep. NJ - Psychology and aging AB - Research has demonstrated the importance of having a purpose in older adulthood; however, little is known about whether and how individuals vary on sense of purpose over time. The current study examined patterns of mean- and individual-level change in purpose among men in the Veterans Affairs Normative Aging Study (n = 587, M(age) = 74 years) across a 3-year span. Findings demonstrate that while little mean-level change was present, there was interindividual variability in change. Further research is needed to understand why these changes occur, as age, health status, and personality failed to predict individual fluctuations in purpose.Copyright (c) 2015 APA, all rights reserved). IL - 0882-7974 DO - http://dx.doi.org/10.1037/pag0000020 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, U.S. Gov't, Non-P.H.S. LG - English <41. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26096497 AU - Tseng CL AU - Chen YT AU - Huang CJ AU - Luo JC AU - Peng YL AU - Huang DF AU - Hou MC AU - Lin HC AU - Lee FY FA - Tseng, C-L FA - Chen, Y-T FA - Huang, C-J FA - Luo, J-C FA - Peng, Y-L FA - Huang, D-F FA - Hou, M-C FA - Lin, H-C FA - Lee, F-Y IN - Tseng,C-L. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Tseng,C-L. Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Chen,Y-T. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Chen,Y-T. Department of Medicine, Taipei City Hospital Heping Fuyou Branch, Taipei, Taiwan. Huang,C-J. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Huang,C-J. Division of Endocrinology and Metabolism, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Luo,J-C. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Luo,J-C. Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Peng,Y-L. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Peng,Y-L. Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Huang,D-F. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Huang,D-F. Division of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Hou,M-C. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Hou,M-C. Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Hou,M-C. Department of Medicine, Endoscopic Center for Diagnosis and Therapy, Taipei Veterans General Hospital, Taipei, Taiwan. Lin,H-C. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Lin,H-C. Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Lee,F-Y. Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Lee,F-Y. Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. TI - Short-term use of glucocorticoids and risk of peptic ulcer bleeding: a nationwide population-based case-crossover study. SO - Alimentary Pharmacology & Therapeutics. 42(5):599-606, 2015 Sep. AS - Aliment Pharmacol Ther. 42(5):599-606, 2015 Sep. NJ - Alimentary pharmacology & therapeutics AB - BACKGROUND: Controversy exists regarding glucocorticoids therapy and the risk of peptic ulcer bleeding (PUB). AB - AIM: The present study was undertaken to determine whether short-term use of glucocorticoids is associated with the occurrence of peptic ulcer bleeding. AB - METHODS: The records of adult patients hospitalised for newly diagnosed peptic ulcer bleeding from 2000 to 2012 were retrieved from the Taiwan National Health Insurance Research Database, a nationwide population-based registry system. The association between systemic glucocorticoids usage and peptic ulcer bleeding was determined with a conditional logistic regression model comparing cases and controls during time windows of 7, 14 and 28 days using a case-crossover design. AB - RESULTS: Of the 8894 enrolled patients, the adjusted self-matched odds ratios for peptic ulcer bleeding after exposure to the glucocorticoids were 1.37 (95% CI: 1.12-1.68, P = 0.003) for the 7-day window, 1.66 (95% CI: 1.38-2.00, P < 0.001) for the 14-day window and 1.84 (95% CI: 1.57-2.16, P < 0.001) for the 28-day window. Moderate to high, but not low dose glucocorticoids (methylprednisolone <4 mg/day or its equivalence) were associated with an increased risk of peptic ulcer bleeding. Concomitant use of a nonselective nonsteroidal anti-inflammatory drug (NSAID) or aspirin further elevated the risk. However, it does not eliminate the effect of underlying diseases flare-up that may have placed the patients at risk for peptic ulcer bleeding in this kind of study design. AB - CONCLUSIONS: Short-term (7-28 days) exposure to glucocorticoids is significantly associated with peptic ulcer bleeding; this risk seems dose-dependent and is higher when nonselective NSAIDs or aspirin are used concurrently.Copyright © 2015 John Wiley & Sons Ltd. IL - 0269-2813 DO - http://dx.doi.org/10.1111/apt.13298 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <42. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25989800 AU - Lee KB AU - Park HW AU - Cho HJ AU - Seon JK FA - Lee, Keun-bae FA - Park, Hyeong-won FA - Cho, Hyun-jong FA - Seon, Jong-keun IN - Lee,Keun-bae. Department of Orthopedic Surgery, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea. kbleeos@chonnam.ac.kr. Park,Hyeong-won. Department of Orthopedic Surgery, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea. Cho,Hyun-jong. Department of Orthopedic Surgery, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea. Seon,Jong-keun. Department of Orthopedic Surgery, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea. TI - Comparison of Arthroscopic Microfracture for Osteochondral Lesions of the Talus With and Without Subchondral Cyst. SO - American Journal of Sports Medicine. 43(8):1951-6, 2015 Aug. AS - Am J Sports Med. 43(8):1951-6, 2015 Aug. NJ - The American journal of sports medicine LM - Available online from MWHC library: 1995 - present, Available in print through MWHC library: 1999 - 2006 AB - BACKGROUND: Although various treatment modalities for an osteochondral lesion of the talus (OLT) with a subchondral cyst have been recommended previously, the primary treatment methods for such conditions have yet to be conclusively determined. Moreover, few comprehensive studies have compared the outcomes of cases where patients were treated with microfracture for OLT with and without subchondral cysts. AB - PURPOSE: To evaluate the clinical outcomes after arthroscopic microfractures performed as a primary treatment for OLT with a subchondral cyst. AB - STUDY DESIGN: Cohort study; Level of evidence, 2. AB - METHODS: The study cohort consisted of 102 patients (102 ankles) who underwent arthroscopic microfracture for small to midsized OLT. The ankles were divided into a cyst group (45 ankles) and a noncyst group (57 ankles).The American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot scale, visual analog scale (VAS), and Ankle Activity Score (AAS) were used to compare the clinical outcomes between the groups over a mean follow-up period of 48 months. AB - RESULTS: The mean AOFAS ankle-hindfoot scores were 64.8 in the cyst group and 66.2 in the noncyst group preoperatively. These improved to 91.8 and 91.3, respectively, at the final follow-up. The mean VAS scores were 7.5 in the cyst group and 7.3 in the noncyst group preoperatively; these improved to 2.3 and 2.2, respectively, at the final follow-up. The mean AAS in the cyst group and the noncyst group improved from 2.7 and 2.6 preoperatively to 6.7 and 6.5 at the final follow-up, respectively. In terms of radiographic stage improvements, the cyst group showed no change in 18 ankles (40%) and showed improvements of 1 grade in 13 ankles (28.9%), 2 grades in 9 ankles (20%), 3 grades in 3 ankles (6.7%), and 4 grades in 2 ankles (4.4%). The noncyst group showed no change in 17 ankles (29.8%) and showed improvements of 1 grade in 11 ankles (19.3%), 2 grades in 11 ankles (19.3%), 3 grades in 14 ankles (24.6%), and 4 grades in 4 ankles (7.0%). No significant differences were found between the groups in terms of the AOFAS score, VAS score, AAS, or radiographic stage improvements. AB - CONCLUSION: OLT with and without subchondral cysts treated with arthroscopic microfracture showed similarly good clinical results. The study results suggest that microfracture could be a primary treatment strategy for treating small to midsized OLT regardless of the existence of subchondral cysts.Copyright © 2015 The Author(s). IL - 0363-5465 DO - http://dx.doi.org/10.1177/0363546515584755 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <43. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26220420 AU - Morales-Garcia C AU - Rodrigo T AU - Garcia-Clemente MM AU - Munoz A AU - Bermudez P AU - Casas F AU - Somoza M AU - Mila C AU - Penas A AU - Hidalgo C AU - Casals M AU - Cayla JA AU - Working Group on Under-reporting of Tuberculosis in Spain FA - Morales-Garcia, Concepcion FA - Rodrigo, Teresa FA - Garcia-Clemente, Marta M FA - Munoz, Ana FA - Bermudez, Pilar FA - Casas, Francisco FA - Somoza, Maria FA - Mila, Celia FA - Penas, Anton FA - Hidalgo, Carmen FA - Casals, Marti FA - Cayla, Joan A FA - Working Group on Under-reporting of Tuberculosis in Spain IN - Morales-Garcia,Concepcion. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. concepcionmorales@telefonica.net. Morales-Garcia,Concepcion. Hospital Universitario Virgen de las Nieves de Granada, Granada, Spain. concepcionmorales@telefonica.net. Rodrigo,Teresa. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. pii_tb_teresa_rodrigo@separ.es. Rodrigo,Teresa. Fundacion Respira de la SEPAR, Barcelona, Spain. pii_tb_teresa_rodrigo@separ.es. Rodrigo,Teresa. Agencia de Salud Publica, Barcelona, Spain. pii_tb_teresa_rodrigo@separ.es. Garcia-Clemente,Marta M. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. mgclemen@gmail.com. Garcia-Clemente,Marta M. Hospital Central de Asturias de Oviedo, Oviedo, Spain. mgclemen@gmail.com. Munoz,Ana. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. anamunozml@gmail.com. Munoz,Ana. Hospital Universitario Carlos Haya de Malaga, Malaga, Spain. anamunozml@gmail.com. Bermudez,Pilar. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. bermudezmp@gmail.com. Bermudez,Pilar. Hospital Universitario Carlos Haya de Malaga, Malaga, Spain. bermudezmp@gmail.com. Casas,Francisco. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. franciscocasas@neumosur.net. Casas,Francisco. Hospital Universitario San Cecilio de Granada, Granada, Spain. franciscocasas@neumosur.net. Somoza,Maria. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. msomoza@cst.cat. Somoza,Maria. Consorcio Sanitario de Tarrasa, Barcelona, Spain. msomoza@cst.cat. Mila,Celia. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. celia.mila@gmail.com. Mila,Celia. Unitat de Prevencio i Control de Tuberculosis de Barcelona, Barcelona, Spain. celia.mila@gmail.com. Penas,Anton. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. anton.penas.truque@sergas.es. Penas,Anton. Hospital Xeral-Calde de Lugo, Lugo, Spain. anton.penas.truque@sergas.es. Hidalgo,Carmen. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. chidalgo72@gmail.com. Hidalgo,Carmen. Hospital Universitario Virgen de las Nieves de Granada, Granada, Spain. chidalgo72@gmail.com. Casals,Marti. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. mcasals@aspb.es. Casals,Marti. Agencia de Salud Publica, Barcelona, Spain. mcasals@aspb.es. Cayla,Joan A. Programa Integrado de Investigacion en Tuberculosis (PII-TB) de la Sociedad Espanola de Neumologia y Cirugia Toracica (SEPAR), Barcelona, Spain. jcayla@aspb.es. Cayla,Joan A. Agencia de Salud Publica, Barcelona, Spain. jcayla@aspb.es. Cayla,Joan A. CIBER de Epidemiologia y Salud Publica (CIBERESP), Barcelona, Spain. jcayla@aspb.es. TI - Factors associated with unreported tuberculosis cases in Spanish hospitals. SO - BMC Infectious Diseases. 15:295, 2015. AS - BMC Infect Dis. 15:295, 2015. NJ - BMC infectious diseases LM - Available online from MWHC library: 2001 - present AB - BACKGROUND: Under-reporting of tuberculosis (TB) cases complicates disease control, hinders contact tracing and alters the accuracy of epidemiological data, including disease burden. The objective of the present study is to evaluate the proportion of unreported TB cases in Spanish healthcare facilities and to identify the associated factors. AB - METHODS: A multi-center retrospective study design was employed. The study included TB cases diagnosed in 16 facilities during 2011-2012. These cases were compared to those reported to the corresponding public health departments. Demographic, microbiological and clinical data were analyzed to determine the factors associated with unreported cases. Associated factors were analyzed on a bivariate level using the x(2) test and on a multivariate level using a logistic regression. Odds ratios (OR) and 95 % confidence intervals (CI) were calculated. AB - RESULTS: Of the 592 TB cases included in the study, 85 (14.4 %) were not reported. The percentage of unreported cases per healthcare center ranged from 0-45.2 %. The following variables were associated to under-reporting at a multivariate level: smear-negative TB (OR = 1.87; CI:1.07-3.28), extrapulmonary disease (OR = 2.07; CI:1.05-4.09) and retired patients (OR = 3.04; CI:1.29-7.18). A nurse case manager was present in all of the centers with 100 % reporting. The percentage of reported cases among the smear-positive cases was 9.4 % and 19.4 % (p = 0.001) among the rest of the study population. Smear-positive TB was no associated to under-reporting. AB - CONCLUSIONS: It is important that TB Control Programs encourage thorough case reporting to improve disease control, contact tracing and accuracy of epidemiological data. The help from a TB nurse case manager could improve the rate of under-reporting. IL - 1471-2334 DO - http://dx.doi.org/10.1186/s12879-015-1047-0 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <44. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26190609 AU - Kim MY AU - Her JY AU - Kim MK AU - Lee KG FA - Kim, Min Yeop FA - Her, Jae-Young FA - Kim, Mina K FA - Lee, Kwang-Geun IN - Kim,Min Yeop. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro-1-gil, Jung-gu, Seoul 100-715, Republic of Korea. Her,Jae-Young. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro-1-gil, Jung-gu, Seoul 100-715, Republic of Korea. Kim,Mina K. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro-1-gil, Jung-gu, Seoul 100-715, Republic of Korea. Lee,Kwang-Geun. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro-1-gil, Jung-gu, Seoul 100-715, Republic of Korea. Electronic address: kwglee@dongguk.edu. TI - Formation and reduction of furan in a soy sauce model system. SO - Food Chemistry. 189:114-9, 2015 Dec 15. AS - FOOD CHEM.. 189:114-9, 2015 Dec 15. NJ - Food chemistry AB - The formation and reduction of furan using a soy sauce model system were investigated in the present study. The concentration of furan fermented up to 30 days increased by 211% after sterilization compared to without sterilization. Regarding fermentation temperature, furan level after 30 days' fermentation was the highest at 30degreeC (86.21 ng/mL). The furan levels in the soy sauce fermentation at 20degreeC and 40degreeC were reduced by 45% and 88%, respectively compared to 30degreeC fermentation. Five metal ions (iron sulfate, zinc sulfate, manganese sulfate, magnesium sulfate, and calcium sulfate), sodium sulfite, ascorbic acid, dibutyl hydroxyl toluene (BHT), and butylated hydroxyanisole (BHA) were added in a soy sauce model system. The addition of metal ions such as magnesium sulfate and calcium sulfate reduced the furan concentration significantly by 36-90% and 27-91%, respectively in comparison to furan level in the control sample (p<0.05). Iron sulfate and ascorbic acid increased the furan level at 30 days' fermentation in the soy sauce model system by 278% and 87%, respectively. In the case of the BHT and BHA, furan formation generally was reduced in the soy sauce model system by 84%, 56%, respectively.Copyright © 2015 Elsevier Ltd. All rights reserved. IL - 0308-8146 DO - http://dx.doi.org/10.1016/j.foodchem.2015.02.015 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <45. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26190608 AU - Kim JS AU - Her JY AU - Lee KG FA - Kim, Jin-Sil FA - Her, Jae-Young FA - Lee, Kwang-Geun IN - Kim,Jin-Sil. Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Chung-gu, Seoul 100-715, Republic of Korea. Her,Jae-Young. Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Chung-gu, Seoul 100-715, Republic of Korea. Lee,Kwang-Geun. Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Chung-gu, Seoul 100-715, Republic of Korea. Electronic address: kwglee@dongguk.edu. TI - Formation and reduction of carcinogenic furan in various model systems containing food additives. SO - Food Chemistry. 189:108-13, 2015 Dec 15. AS - FOOD CHEM.. 189:108-13, 2015 Dec 15. NJ - Food chemistry AB - The aim of this study was to analyse and reduce furan in various model systems. Furan model systems consisting of monosaccharides (0.5M glucose and ribose), amino acids (0.5M alanine and serine) and/or 1.0M ascorbic acid were heated at 121degreeC for 25 min. The effects of food additives (each 0.1M) such as metal ions (iron sulphate, magnesium sulphate, zinc sulphate and calcium sulphate), antioxidants (BHT and BHA), and sodium sulphite on the formation of furan were measured. The level of furan formed in the model systems was 6.8-527.3 ng/ml. The level of furan in the model systems of glucose/serine and glucose/alanine increased 7-674% when food additives were added. In contrast, the level of furan decreased by 18-51% in the Maillard reaction model systems that included ribose and alanine/serine with food additives except zinc sulphate.Copyright © 2014 Elsevier Ltd. All rights reserved. IL - 0308-8146 DO - http://dx.doi.org/10.1016/j.foodchem.2014.10.128 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <46. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26190603 AU - Kim YG AU - Lyu J AU - Kim MK AU - Lee KG FA - Kim, Yong Gun FA - Lyu, Jihye FA - Kim, Mina K FA - Lee, Kwang-Geun IN - Kim,Yong Gun. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro 1gil, Jung-gu, Seoul 100-715, Republic of Korea. Lyu,Jihye. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro 1gil, Jung-gu, Seoul 100-715, Republic of Korea. Kim,Mina K. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro 1gil, Jung-gu, Seoul 100-715, Republic of Korea. Lee,Kwang-Geun. Department of Food Science and Biotechnology, Dongguk University-Seoul, 30, Pildong-ro 1gil, Jung-gu, Seoul 100-715, Republic of Korea. Electronic address: kwglee@dongguk.edu. TI - Effect of citrulline, urea, ethanol, and urease on the formation of ethyl carbamate in soybean paste model system. SO - Food Chemistry. 189:74-9, 2015 Dec 15. AS - FOOD CHEM.. 189:74-9, 2015 Dec 15. NJ - Food chemistry AB - The aim of this study was to determine the effect of urease on the formation of ethyl carbamate (EC) in the presence of previously known precursors of EC (citrulline, urea, and ethanol) using a soybean paste model system. The levels of EC were quantitatively determined by gas chromatography-mass spectrometry (GC-MS) every five days for a 30-day period. After 30 days fermentation, the concentration of EC increased significantly by 135.2%, 242.2%, and 3757.1% when the precursors (citrulline, urea and ethanol) were added to the model system, respectively (p<0.05). Urease significantly decreased the level of EC by 38.4%, 18.8%, and 17.3% when citrulline, urea, and ethanol were added to the model system, respectively (p<0.05).Copyright © 2015 Elsevier Ltd. All rights reserved. IL - 0308-8146 DO - http://dx.doi.org/10.1016/j.foodchem.2015.02.012 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <47. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26190599 AU - Lee J AU - Her JY AU - Lee KG FA - Lee, Jongin FA - Her, Jae-Young FA - Lee, Kwang-Geun IN - Lee,Jongin. Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Jung-gu, Seoul 100-715, Republic of Korea. Her,Jae-Young. Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Jung-gu, Seoul 100-715, Republic of Korea. Lee,Kwang-Geun. Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Jung-gu, Seoul 100-715, Republic of Korea. Electronic address: kwglee@dongguk.edu. TI - Reduction of aflatoxins (B1, B2, G1, and G2) in soybean-based model systems. SO - Food Chemistry. 189:45-51, 2015 Dec 15. AS - FOOD CHEM.. 189:45-51, 2015 Dec 15. NJ - Food chemistry AB - The effects of chemical, physical, and cooking treatments on the reduction of aflatoxin B1 (AFB1), B2, G1, and G2 in soybean matrix were investigated. A HPLC-FLD with a Kobra cell system was used for the quantitative analysis of aflatoxins (AFs). To decrease the level of AFs during the soaking process, the contaminated soybeans were submerged in organic acid solutions. The reduction rates of AFB1 in 1.0N citric acid, lactic acid, succinic acid, and tartaric acid for 18h were 94.1%, 92.7%, 62.0%, and 95.1%, respectively. In the case of pH and autoclave treatment, the level of AFB1 was significantly decreased during autoclaving process at pH 7.4, 9.0, and 11.1, compared with the non-autoclaved samples (p<0.05). In the case of physical treatment, the heating process at 100 and 150degreeC for 90min significantly decreased the level of AFB1 by 41.9% and 81.2%, respectively (p<0.05). The reduction rate of AFB1 after cooking was 97.9% for soybean milk and 33.6% for steamed soybeans. Copyright © 2015 Elsevier Ltd. All rights reserved. IL - 0308-8146 DO - http://dx.doi.org/10.1016/j.foodchem.2015.02.013 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <48. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26081439 AU - Shanqimuge AU - Liang H AU - Zhang C AU - Shao C AU - Peng X AU - Liang L AU - Su J AU - Li C FA - Shanqimuge FA - Liang, Huizhen FA - Zhang, Changxia FA - Shao, Chunfu FA - Peng, Xiaopei FA - Liang, Liquan FA - Su, Jing FA - Li, Changwen IN - Shanqimuge,. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. Liang,Huizhen. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. Zhang,Changxia. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. Shao,Chunfu. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. Peng,Xiaopei. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. Liang,Liquan. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. Su,Jing. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. Li,Changwen. Div. of Nutraceutical Products, Tasly Holding Group, Tianjin, 300040, China. TI - A DGGE Marker-Mediated Fast Monitoring of Bacterial Diversity and Comprehensive Identification of High-Temperature Daqu Starter. SO - Journal of Food Science. 80(7):M1519-25, 2015 Jul. AS - J Food Sci. 80(7):M1519-25, 2015 Jul. NJ - Journal of food science AB - UNLABELLED: Bacteria play an essential role in Daqu starter (Daqu) fermentation. The identification of Daqu bacteria was investigated by polymerase chain reaction (PCR) based denaturing gradient gel electrophoresis (DGGE) analysis of the highly variable V3 region of the 16S rRNA gene. Here, we define a novel DGGE marker for the quick identification of Daqu bacteria. A dynamic alteration of the bacterial populations at different stages of fermentation was determined through a 2-y continuous monitoring. The physicochemical parameters of Daqu at different fermentation stages were investigated by weighing, NaOH titration, and HCl hydrolysis together with Fehling reagent methods. Furthermore, infrared spectral analysis using Fourier Transformed Infrared Spectroscopy was performed to determine physicochemical changes of Daqu. Therefore, our studies provide key insight for a comprehensive quality control of Daqu at different fermentation stages using the PCR-DGGE analysis combined with the physicochemical measurement. AB - PRACTICAL APPLICATION: Chinese liquor is one of the 6 well-known distilled spirits in the world. High-temperature Daqu acts as an important source of nutrients and of microorganisms in the solid-state fermentation of Chinese Moutai-flavor liquor, which has a critical impact on the final flavor of the liquor. The study identifies a novel DGGE marker and provides an efficient way to identify bacterial diversity in Daqu from different fermentation stages. Importantly, the study defines dynamic changes of the physicochemical parameters and the infrared spectra analysis of Daqu during the fermentation process. These studies will help to (1) establish a standard operation procedure for Daqu production; (2) stabilize manufacturing process for Daqu fermentation and even for liquor brewing.Copyright © 2015 Institute of Food Technologists IL - 0022-1147 DO - http://dx.doi.org/10.1111/1750-3841.12903 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <49. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26018816 AU - Hong J AU - Kim Y AU - Kim J AU - Heu S AU - Kim SR AU - Kim KP AU - Roh E FA - Hong, Jisoo FA - Kim, Yangkyun FA - Kim, Jonguk FA - Heu, Sunggi FA - Kim, Se-ri FA - Kim, Kwang-Pyo FA - Roh, Eunjung IN - Hong,Jisoo. Microbial Safety Team, Natl. Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea. Kim,Yangkyun. Microbial Safety Team, Natl. Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea. Kim,Jonguk. Microbial Safety Team, Natl. Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea. Heu,Sunggi. Microbial Safety Team, Natl. Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea. Kim,Se-ri. Microbial Safety Team, Natl. Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea. Kim,Kwang-Pyo. Dept. of Food Science, School of Agriculture and Life Sciences, Chonbuk Natl. Univ, Jeonju, Chonbuk 561-756, Republic of Korea. Roh,Eunjung. Microbial Safety Team, Natl. Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea. TI - Genetic Diversity and Antibiotic Resistance Patterns of Staphylococcus Aureus Isolated from Leaf Vegetables in Korea. SO - Journal of Food Science. 80(7):M1526-31, 2015 Jul. AS - J Food Sci. 80(7):M1526-31, 2015 Jul. NJ - Journal of food science AB - Staphylococcus aureus is an important foodborne pathogen on global basis. The current study investigated the genetic patterns in S. aureus isolates from leaf vegetables (n = 53). Additional isolates from livestock (n = 31) and humans (n = 27) were compared with the leaf vegetable isolates. Genes associated with toxins, antibiotic resistance, and pulsed-field gel electrophoresis (PFGE) patterns were analyzed. At least 1 enterotoxin-encoding gene (sea, seb, sec, sed, and see) was detected in 11 of 53 (20.75%) leaf vegetable isolates. When the agr (accessory gene regulator) grouping was analyzed, agr II was the major group, whereas agr IV was not present in leaf vegetable isolates. All S. aureus isolates from leaf vegetables were resistant to more than one of the antibiotics tested. Nineteen of 53 (35.85%) isolates from leaf vegetables exhibited multidrug-resistance, and 11 of these were MRSA (methicillin-resistant S. aureus). A dendrogram displaying the composite types of S. aureus isolates from 3 origins was generated based on the combination of the toxin genes, agr genes, antibiotic resistance, and PFGE patterns. The isolates could be clustered into 8 major composite types. The genetic patterns of S. aureus isolates from leaf vegetables and humans were similar, whereas those from livestock had unique patterns. This suggests some S. aureus isolates from leaf vegetables to be of human origin. Copyright © 2015 Institute of Food Technologists IL - 0022-1147 DO - http://dx.doi.org/10.1111/1750-3841.12909 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <50. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25981189 AU - Chen W AU - Wang H FA - Chen, Weishan FA - Wang, Hsiuying IN - Chen,Weishan. Institute of Statistics, National Chiao Tung University, Hsinchu, Taiwan. Wang,Hsiuying. Institute of Statistics, National Chiao Tung University, Hsinchu, Taiwan. Electronic address: wang@stat.nctu.edu.tw. TI - Variance estimation for nucleotide substitution models. SO - Molecular Phylogenetics & Evolution. 90:97-103, 2015 Sep. AS - Mol Phylogenet Evol. 90:97-103, 2015 Sep. NJ - Molecular phylogenetics and evolution AB - The current variance estimators for most evolutionary models were derived when a nucleotide substitution number estimator was approximated with a simple first order Taylor expansion. In this study, we derive three variance estimators for the F81, F84, HKY85 and TN93 nucleotide substitution models, respectively. They are obtained using the second order Taylor expansion of the substitution number estimator, the first order Taylor expansion of a squared deviation and the second order Taylor expansion of a squared deviation, respectively. These variance estimators are compared with the existing variance estimator in terms of a simulation study. It shows that the variance estimator, which is derived using the second order Taylor expansion of a squared deviation, is more accurate than the other three estimators. In addition, we also compare these estimators with an estimator derived by the bootstrap method. The simulation shows that the performance of this bootstrap estimator is similar to the estimator derived by the second order Taylor expansion of a squared deviation. Since the latter one has an explicit form, it is more efficient than the bootstrap estimator. Copyright © 2015 Elsevier Inc. All rights reserved. IL - 1055-7903 DO - http://dx.doi.org/10.1016/j.ympev.2015.05.003 PT - Journal Article LG - English <51. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26152211 AU - Lee K AU - Lee B AU - Lee MH AU - Kim B AU - Chinannai KS AU - Ham I AU - Choi HY FA - Lee, Kyungjin FA - Lee, Byonghee FA - Lee, Mi-Hwa FA - Kim, Bumjung FA - Chinannai, Khanita Suman FA - Ham, Inhye FA - Choi, Ho-Young IN - Lee,Kyungjin. Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. dostudy@naver.com. Lee,Byonghee. Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. opharm@korea.kr. Lee,Mi-Hwa. Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. mythio@naver.com. Kim,Bumjung. Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. ori-pharm@daum.net. Chinannai,Khanita Suman. Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. sumankhanita6@gmail.com. Ham,Inhye. Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. iham@khu.ac.kr. Choi,Ho-Young. Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. hychoi@khu.ac.kr. TI - Effect of Ampelopsis Radix on wound healing in scalded rats. SO - BMC Complementary & Alternative Medicine. 15:213, 2015. AS - BMC Altern Med. 15:213, 2015. NJ - BMC complementary and alternative medicine LM - Available online from MWHC library: 2001 - present AB - BACKGROUND: Ampelopsis Radix has been used as a traditional Korean medicine for the treatment of burns and scalds. However, there has been no scientific research to date on the wound healing properties of Ampelopsis Radix for scald burns. This study aimed to evaluate the healing effect of Ampelopsis japonica root tuber ethanol extract (AJE) on induced cutaneous scald injury in Sprague Dawley (SD) rats. AB - METHODS: Hot water scalds were induced in SD rats, who were then divided into the following 5 groups; 1) control group without treatment, 2) positive control group with 1% Silver sulfadiazine (SSD), 3) Vaseline group, and groups 4) and 5) that used Vaseline containing 5% and 20% AJE, respectively. The ointment was applied topically to the experimental rats, once daily for 21 days, starting at 24 h post induction of the scald injury. Gross examination, measurement of wound size, and histopathological examination were performed. And quantitative measurement of cytokine levels of tumor necrosis factor alpha (TNF-alpha), interleukin-10 (IL-10), transforming growth factor beta 1 (TGF-beta1), and vascular endothelial growth factor (VEGF) were performed by enzyme-linked immunosorbent assay. AB - RESULTS: Clinical evaluation showed that the AJE and Vaseline groups, rapidly desquamated scab on day 12 post-scalding; in particular, the 20% AJE group achieved the greatest extent of skin recovery. Sizes of scald wound were significantly lower on days 12, 15, 18, and 21 in the AJE treated groups compared to the control groups. Histopathological evaluation showed a well-organized epithelial layer, angiogenesis, tissue granulation and collagen formation with the exception of inflammatory cells in the AJE-treated groups compared to the control groups on day 14, indicating that tissue regeneration had occurred. AJE treatment decreased TNF-alpha and increased IL-10 levels on days 2 and 14, indicating the anti-inflammatory action of AJE. The AJE groups also showed a decrease in TGF-beta1 levels on day 7 and VEGF on day 14 in the serum of scald inflicted SD rat model. AB - CONCLUSIONS: These results suggest that AJE possesses scald wound healing activity via accelerating the scald wound repair during the inflammation and proliferative phases of the healing process. IL - 1472-6882 DO - http://dx.doi.org/10.1186/s12906-015-0751-z PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <52. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26136077 AU - Kim JS AU - Ku B AU - Woo TG AU - Oh AY AU - Jung YS AU - Soh YM AU - Yeom JH AU - Lee K AU - Park BJ AU - Oh BH AU - Ha NC FA - Kim, J-S FA - Ku, B FA - Woo, T-G FA - Oh, A-Y FA - Jung, Y-S FA - Soh, Y-M FA - Yeom, J-H FA - Lee, K FA - Park, B-J FA - Oh, B-H FA - Ha, N-C IN - Kim,J-S. Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. Ku,B. 1] Department of Biological Sciences, KAIST Institute for the Biocentury, Cancer Metastasis Control Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea [2] Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. Woo,T-G. Department of Molecular Biology, Pusan National University, Busan, Republic of Korea. Oh,A-Y. Department of Molecular Biology, Pusan National University, Busan, Republic of Korea. Jung,Y-S. Department of Molecular Biology, Pusan National University, Busan, Republic of Korea. Soh,Y-M. Department of Biological Sciences, KAIST Institute for the Biocentury, Cancer Metastasis Control Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. Yeom,J-H. Department of Life Science, Chung-Ang University, Seoul, Republic of Korea. Lee,K. Department of Life Science, Chung-Ang University, Seoul, Republic of Korea. Park,B-J. Department of Molecular Biology, Pusan National University, Busan, Republic of Korea. Oh,B-H. Department of Biological Sciences, KAIST Institute for the Biocentury, Cancer Metastasis Control Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. Ha,N-C. Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. TI - Conversion of cell-survival activity of Akt into apoptotic death of cancer cells by two mutations on the BIM BH3 domain. SO - Cell Death & Disease. 6:e1804, 2015. AS - Cell Death Dis. 6:e1804, 2015. NJ - Cell death & disease AB - Survival and proliferation of cancer cells are often associated with hyperactivity of the serine/threonine kinase, Akt. Herein, we show that prosurvival activity of Akt can be converted into prodeath activity by embedding an Akt recognition sequence in the apoptogenic BH3 domain of human BIM. The recognition sequence was created by introducing two mutations, I155R and E158S, into the core region of the BIM BH3 domain. Although a 21-mer BIM BH3 peptide containing these two mutations bound weakly to BCL-XL and BCL-2, this peptide with phosphorylation of Ser158 bound to these proteins with a dissociation constant of <10nM. The crystal structure of the phosphorylated peptide bound to BCL-XL revealed that the phospho-Ser158 makes favorable interactions with two BCL-XL residues, which cannot be formed with unphosphorylated Ser158. Remarkably, the designed peptide showed a cytotoxic effect on PTEN-null PC3 tumor cells whose Akt activity is aberrantly high. The cell-killing activity disappeared when the cellular Akt activity was lowered by ectopic PTEN expression. Thus, these results lay a foundation for developing a peptide or protein agent that is dormant in normal cells but is transformed into a potent apoptogenic molecule upon phosphorylation by hyperactivity of Akt in cancer cells. DO - http://dx.doi.org/10.1038/cddis.2015.118 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <53. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26136075 AU - Liu YW AU - Sun M AU - Xia R AU - Zhang EB AU - Liu XH AU - Zhang ZH AU - Xu TP AU - De W AU - Liu BR AU - Wang ZX FA - Liu, Y-w FA - Sun, M FA - Xia, R FA - Zhang, E-b FA - Liu, X-h FA - Zhang, Z-h FA - Xu, T-p FA - De, W FA - Liu, B-r FA - Wang, Z-x IN - Liu,Y-w. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China. Sun,M. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China. Xia,R. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China. Zhang,E-b. Department of Oncology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. Liu,X-h. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China. Zhang,Z-h. Department of Pathology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. Xu,T-p. Department of Pathology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. De,W. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China. Liu,B-r. Department of Oncology, Affiliated Drum Tower Hospital of Nanjing University, Nanjing, Jiangsu, PR China. Wang,Z-x. Department of Oncology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. TI - LincHOTAIR epigenetically silences miR34a by binding to PRC2 to promote the epithelial-to-mesenchymal transition in human gastric cancer. SO - Cell Death & Disease. 6:e1802, 2015. AS - Cell Death Dis. 6:e1802, 2015. NJ - Cell death & disease AB - lncRNAs play important roles in the epigenetic regulation of carcinogenesis and progression. Previous studies suggest that HOTAIR contributes to gastric cancer (GC) development, and the overexpression of HOTAIR predicts a poor prognosis. In this study, we found that HOTAIR was more highly expressed in diffuse-type GC than in intestinal type (P=0.048). In the diffuse type, there is significant relationship between HOTAIR expression and DFS (P<0.001). CDH1 was downregulated in diffuse-type GC tissues (P=0.0007) and showed a negative relationship with HOTAIR (r(2)=0.154, P=0.0354). In addition, HOTAIR knockdown significantly repressed migration, invasion and metastasis both in vitro and vivo and reversed the epithelial-to-mesenchymal transition in GC cells. We also showed that HOTAIR recruiting and binding to PRC2 epigenetically represses miR34a, which controls the targets C-Met (HGF/C-Met/Snail pathway) and Snail, thus contributing to GC cell-EMT process and accelerating tumor metastasis. Moreover, it is demonstrated that HOTAIR crosstalk with microRNAs during epigenetic regulation. Our results suggest that HOTAIR acts as an EMT regulator and may be a candidate prognostic biomarker and a target for new therapies in GC patients. DO - http://dx.doi.org/10.1038/cddis.2015.150 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <54. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26136074 AU - Ju SY AU - Huang CY AU - Huang WC AU - Su Y FA - Ju, S-Y FA - Huang, C-Y F FA - Huang, W-C FA - Su, Y IN - Ju,S-Y. Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei, Taiwan. Huang,C-Y F. Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei, Taiwan. Huang,W-C. Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei, Taiwan. Su,Y. Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei, Taiwan. TI - Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells. SO - Cell Death & Disease. 6:e1801, 2015. AS - Cell Death Dis. 6:e1801, 2015. NJ - Cell death & disease AB - Accumulating evidence shows that colorectal cancer stem cells (CRSCs) are largely responsible for the metastasis and relapse of colorectal cancer (CRC) after therapy. Hence, identifying new agents that specifically target CRSCs would help improve the effectiveness of current CRC therapies. To accelerate identification of agents targeting CRSCs, the Connectivity Map (CMap) approach was used. Among the top-ranked candidates, thiostrepton, a thiazole antibiotic, was selected for further investigation because of its known tumoricidal activity. Thiostrepton could selectively induce apoptosis in CRSC subpopulations in both parental HCT-15 and HT-29 human CRC lines as well as in EMT and chemoresistant clones derived from them. Further, we investigated its inhibitory effects on the sphere- and colony-forming capabilities of the aforementioned CRC lines. The in vitro inhibition of sphere and colony formation was associated with downregulation of various modulators of the stem cell phenotype. The combination of thiostrepton and oxaliplatin eradicated both CD44(+) HCT-15 and HT-29 cells more efficiently than either drug alone. FoxM1, an oncogenic transcription factor, was identified as a critical positive modulator of stemness and as the main target of thiostrepton in the CRC lines. This is the first report showing the selective killing of CRSCs by thiostrepton, which has been proposed to be a promising anti-neoplastic agent. On the basis of its synergism with oxaliplatin in killing CRSCs in vitro, if this activity is confirmed in vivo, thiostrepton may be a promising agent to be used clinically in combination with current chemotherapies to improve the efficacy of these regimens. DO - http://dx.doi.org/10.1038/cddis.2015.155 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <55. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26032727 AU - Zhang L AU - Liu W AU - Mao F AU - Zhu J AU - Dong G AU - Jiang H AU - Sheng C AU - Miao L AU - Huang L AU - Li J FA - Zhang, Lingling FA - Liu, Wei FA - Mao, Fei FA - Zhu, Jin FA - Dong, Guoqiang FA - Jiang, Hualiang FA - Sheng, Chunquan FA - Miao, Liyan FA - Huang, Lixin FA - Li, Jian IN - Zhang,Lingling. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, China. Liu,Wei. Department of Orthopaedic, The First Affiliated Hospital of Soochow University, Suzhou, China. Mao,Fei. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, China. Zhu,Jin. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, China. Dong,Guoqiang. School of Pharmacy, Second Military Medical University, Shanghai, China. Jiang,Hualiang. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, China. Sheng,Chunquan. School of Pharmacy, Second Military Medical University, Shanghai, China. Miao,Liyan. Department of Clinical Pharmacology Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China. Huang,Lixin. Department of Orthopaedic, The First Affiliated Hospital of Soochow University, Suzhou, China. Li,Jian. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, China. TI - Discovery of benzylidene derivatives as potent Syk inhibitors: synthesis, SAR analysis, and biological evaluation. SO - Archiv der Pharmazie. 348(7):463-74, 2015 Jul. AS - Arch Pharm (Weinheim). 348(7):463-74, 2015 Jul. NJ - Archiv der Pharmazie AB - Four scaffolds of varied benzylidene derivatives were synthesized and evaluated as Syk inhibitors for the treatment of rheumatoid arthritis (RA). Among these 31 compounds, 3-benzylidene pyrrolidine-2,5-dione derivatives (including 12k) universally showed good Syk inhibitory activities in the low micromolar to submicromolar range. In the cellular profiling, compound 12k, the most efficient compound, showed excellent antiproliferative activity against fibroblast-like synoviocytes (FLS)-RA, and demonstrated potencies for suppression of IL-6 and MMP-3 secretion almost equal to R406 (positive control). The oral efficacy of 12k in the murine collagen-induced arthritis model was significant, despite being weaker than R406. Taken together, all preliminary pharmacological results supported 12k as a potential small-molecule inhibitor targeting Syk for the treatment of RA. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. IL - 0365-6233 DO - http://dx.doi.org/10.1002/ardp.201500096 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <56. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26113134 AU - Alrayes N AU - Mohamoud HS AU - Jelani M AU - Ahmad S AU - Vadgama N AU - Bakur K AU - Simpson M AU - Al-Aama JY AU - Nasir J FA - Alrayes, Nuha FA - Mohamoud, Hussein Sheikh Ali FA - Jelani, Musharraf FA - Ahmad, Saleem FA - Vadgama, Nirmal FA - Bakur, Khadijah FA - Simpson, Michael FA - Al-Aama, Jumana Yousuf FA - Nasir, Jamal IN - Alrayes,Nuha. Princess Al-Jawhara Albrahim Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah, 80205, Kingdom of Saudi Arabia. nuharayes@gmail.com. Alrayes,Nuha. Division of Biomedical Sciences (BMS), Human Genetics Research Center, St. George's University of London (SGUL), London, SW17 0RE, UK. nuharayes@gmail.com. Mohamoud,Hussein Sheikh Ali. Princess Al-Jawhara Albrahim Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah, 80205, Kingdom of Saudi Arabia. husseinsheekh@yahoo.co.uk. Mohamoud,Hussein Sheikh Ali. Division of Biomedical Sciences (BMS), Human Genetics Research Center, St. George's University of London (SGUL), London, SW17 0RE, UK. husseinsheekh@yahoo.co.uk. Jelani,Musharraf. Princess Al-Jawhara Albrahim Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah, 80205, Kingdom of Saudi Arabia. musharraf_jelani@yahoo.com. Jelani,Musharraf. Medical Genetics and Molecular Biology Unit, Biochemistry Department, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, 25000, Pakistan. musharraf_jelani@yahoo.com. Ahmad,Saleem. Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. sahmad@yahoo.com. Vadgama,Nirmal. Division of Biomedical Sciences (BMS), Human Genetics Research Center, St. George's University of London (SGUL), London, SW17 0RE, UK. nvadgama@sgul.ac.uk. Bakur,Khadijah. Princess Al-Jawhara Albrahim Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah, 80205, Kingdom of Saudi Arabia. kbakur@yahoo.com. Simpson,Michael. Genetics and Molecular Medicine, King's College London, Guy's Hospital, London, SE1 9RT, UK. michael.simpson@kcl.ac.uk. Al-Aama,Jumana Yousuf. Princess Al-Jawhara Albrahim Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah, 80205, Kingdom of Saudi Arabia. al-aama@yahoo.com. Al-Aama,Jumana Yousuf. Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. al-aama@yahoo.com. Nasir,Jamal. Division of Biomedical Sciences (BMS), Human Genetics Research Center, St. George's University of London (SGUL), London, SW17 0RE, UK. p1106394@sgul.ac.uk. TI - Truncating mutation in intracellular phospholipase A1 gene (DDHD2) in hereditary spastic paraplegia with intellectual disability (SPG54). SO - BMC Research Notes. 8:271, 2015. AS - BMC Res Notes. 8:271, 2015. NJ - BMC research notes AB - BACKGROUND: Hereditary spastic paraplegias (HSP), a group of genetically heterogeneous neurological disorders with more than 56 documented loci (SPG1-56), are described either as uncomplicated (or pure), or complicated where in addition to spasticity and weakness of lower extremeties, additional neurological symptoms are present, including dementia, loss of vision, epilepsy, mental retardation and ichthyosis. We identified a large consanguineous family of Indian descent with four affected members with childhood onset HSP (SPG54), presenting with upper and lower limb spasticity, mental retardation and agenesis of the corpus callosum. AB - RESULTS: A common region of homozygosity on chromosome 8 spanning seven megabases (Mb) was identified in the affected individuals using the Illumina human cytoSNP-12 DNA Analysis BeadChip Kit. Exome sequencing identified a homozygous stop gain mutation (pR287X) in the phospholipase A1 gene DDHD2, in the affected individuals, resulting in a premature stop codon and a severely truncated protein lacking the SAM and DDHD domains crucial for phosphoinositide binding and phospholipase activity. AB - CONCLUSION: This mutation adds to the knowledge of HSP, suggests a possible founder effect for the pR287X mutation, and adds to the list of genes involved in lipid metabolism with a role in HSP and other neurodegenerative disorders. IL - 1756-0500 DO - http://dx.doi.org/10.1186/s13104-015-1227-4 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <57. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26109118 AU - Charan J AU - Chaudhari M AU - Jackson R AU - Mhaskar R AU - Reljic T AU - Kumar A FA - Charan, Jaykaran FA - Chaudhari, Mayur FA - Jackson, Ryan FA - Mhaskar, Rahul FA - Reljic, Tea FA - Kumar, Ambuj IN - Charan,Jaykaran. Department of Pharmacology, GMERS Medical College, Patan, Gujarat, India. Chaudhari,Mayur. Department of Pharmacology, Government Medical College, Surat, Gujarat, India. Jackson,Ryan. Department of Otolaryngology Head and Neck Surgery, Morsani College of Medicine, USF, Tampa, Florida, USA. Mhaskar,Rahul. Division of EBM, Morsani College of Medicine, Internal Medicine, Tampa, Florida, USA. Reljic,Tea. Division of EBM, Morsani College of Medicine, Internal Medicine, Tampa, Florida, USA. Kumar,Ambuj. Division of EBM, Morsani College of Medicine, Internal Medicine, Tampa, Florida, USA. TI - Comparison of methodological quality of positive versus negative comparative studies published in Indian medical journals: a systematic review. [Review] SO - BMJ Open. 5(6):e007853, 2015. AS - BMJ Open. 5(6):e007853, 2015. NJ - BMJ open AB - OBJECTIVES: Published negative studies should have the same rigour of methodological quality as studies with positive findings. However, the methodological quality of negative versus positive studies is not known. The objective was to assess the reported methodological quality of positive versus negative studies published in Indian medical journals. AB - DESIGN: A systematic review (SR) was performed of all comparative studies published in Indian medical journals with a clinical science focus and impact factor >1 between 2011 and 2013. The methodological quality of randomised controlled trials (RCTs) was assessed using the Cochrane risk of bias tool, and the Newcastle-Ottawa scale for observational studies. The results were considered positive if the primary outcome was statistically significant and negative otherwise. When the primary outcome was not specified, we used data on the first outcome reported in the history followed by the results section. Differences in various methodological quality domains between positive versus negative studies were assessed by Fisher's exact test. AB - RESULTS: Seven journals with 259 comparative studies were included in this SR. 24% (63/259) were RCTs, 24% (63/259) cohort studies, and 49% (128/259) case-control studies. 53% (137/259) of studies explicitly reported the primary outcome. Five studies did not report sufficient data to enable us to determine if results were positive or negative. Statistical significance was determined by p value in 78.3% (199/254), CI in 2.8% (7/254), both p value and CI in 11.8% (30/254), and only descriptive in 6.3% (16/254) of studies. The overall methodological quality was poor and no statistically significant differences between reporting of methodological quality were detected between studies with positive versus negative findings. AB - CONCLUSIONS: There was no difference in the reported methodological quality of positive versus negative studies. However, the uneven reporting of positive versus negative studies (72% vs 28%) indicates a publication bias in Indian medical journals with an impact factor of >1.Copyright Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions. DO - http://dx.doi.org/10.1136/bmjopen-2015-007853 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Review LG - English <58. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26072055 AU - Huang CM AU - Huang MY AU - Tang JY AU - Chen SC AU - Wang LY AU - Lin ZY AU - Huang CJ FA - Huang, Chun-Ming FA - Huang, Ming-Yii FA - Tang, Jen-Yang FA - Chen, Shinn-Cherng FA - Wang, Liang-Yen FA - Lin, Zu-Yau FA - Huang, Chih-Jen IN - Huang,Chun-Ming. Department of Radiation Oncology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung, Taiwan. 930321@ms.kmuh.org.tw. Huang,Chun-Ming. Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. 930321@ms.kmuh.org.tw. Huang,Ming-Yii. Department of Radiation Oncology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung, Taiwan. miyihu@kmu.edu.tw. Huang,Ming-Yii. Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. miyihu@kmu.edu.tw. Huang,Ming-Yii. Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. miyihu@kmu.edu.tw. Tang,Jen-Yang. Department of Radiation Oncology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung, Taiwan. reyata@gmail.com. Tang,Jen-Yang. Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. reyata@gmail.com. Chen,Shinn-Cherng. Division of Hepatobiliary Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. chshch@kmu.edu.tw. Chen,Shinn-Cherng. Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. chshch@kmu.edu.tw. Wang,Liang-Yen. Division of Hepatobiliary Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. liyewa@kmu.edu.tw. Wang,Liang-Yen. Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. liyewa@kmu.edu.tw. Lin,Zu-Yau. Division of Hepatobiliary Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. 940109@ms.kmuh.org.tw. Lin,Zu-Yau. Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. 940109@ms.kmuh.org.tw. Huang,Chih-Jen. Department of Radiation Oncology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung, Taiwan. ccjjhh@kmu.edu.tw. Huang,Chih-Jen. Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. ccjjhh@kmu.edu.tw. Huang,Chih-Jen. Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. ccjjhh@kmu.edu.tw. TI - Feasibility and efficacy of helical tomotherapy in cirrhotic patients with unresectable hepatocellular carcinoma. SO - World Journal of Surgical Oncology. 13:201, 2015. AS - World J Surg Oncol. 13:201, 2015. NJ - World journal of surgical oncology LM - Available online through MWHC library: 2003 - present AB - BACKGROUND: This study is to evaluate the toxicity and outcomes of helical tomotherapy (HT) in patients treated for unresectable hepatocellular carcinoma (HCC). AB - METHODS: From March 2008 to September 2010, 38 patients with unresectable HCC were treated with HT. The median patient age was 67 years (range, 45-85). The median follow-up period was 17.2 months (range, 7-46). All patients had liver cirrhosis. Median radiation dose was 54 Gy (range, 46-71.8) delivered in 1.8 to 2.4-Gy fractions. The planning target volumes were 241.2+/-153.1 cm(3) (range, 45.8-722.4). Treatment responses were assessed in 3-6 months after HT. AB - RESULTS: There was a complete response in 2 patients (5.2 %), partial response in 18 patients (47.4 %), stable disease in 13 patients (34.2 %), and progressive disease in 5 patients (13.2 %). The median overall survival was 12.6 months, and 1- and 2-year overall survival rates were 56.2 and 31.7 %, respectively. Eastern Cooperative Oncology Group (ECOG score, p=0.008), Child-Pugh classification (p=0.012), albumin (p=0.046), and hemoglobin (p=0.028) were significant parameters that predicted primary tumor response to radiotherapy in multivariate analysis. ECOG score (p=0.012), Child-Pugh class (p=0.026), and response to radiotherapy (p=0.016) were independent prognostic factors for overall survival in multivariate analysis. Responders had better overall survival than non-responders (23.6 vs. 5.8 months, p<0.001). The 1- and 2-year overall survival rates for responders were 68.3 and 57 %, respectively, while for non-responders, they were 0 %. The 1- and 2-year local control rates were 88.2 and 82.3 %, respectively. Five patients (13.2 %) had grade 3 or greater liver toxicity, and one patient (2.6 %) had a grade 3 gastric ulcer. No treatment-related liver failure or death was documented in this study. AB - CONCLUSIONS: Radiotherapy using HT seems to be a safe and effective treatment option for unresectable HCC patients. This study indicates that HT is a feasible treatment even in patients without good performance status and hepatic function reservation. IL - 1477-7819 DO - http://dx.doi.org/10.1186/s12957-015-0611-9 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <59. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26086962 AU - Kim JA AU - Ha S AU - Shin KY AU - Kim S AU - Lee KJ AU - Chong YH AU - Chang KA AU - Suh YH FA - Kim, J A FA - Ha, S FA - Shin, K Y FA - Kim, S FA - Lee, K J FA - Chong, Y H FA - Chang, K-A FA - Suh, Y-H IN - Kim,J A. Department of Pharmacology, College of Medicine, Neuroscience Research Institute, MRC, Seoul National University, Seoul, 110-799, Korea. Ha,S. Department of Pharmacology, College of Medicine, Neuroscience Research Institute, Gachon University, Incheon, 405-760, Korea. Shin,K Y. Department of Pharmacology, College of Medicine, Neuroscience Research Institute, MRC, Seoul National University, Seoul, 110-799, Korea. Kim,S. Department of Pharmacology, College of Medicine, Neuroscience Research Institute, Gachon University, Incheon, 405-760, Korea. Lee,K J. Synaptic Circuit Plasticity Laboratory, Department of Structure & Function of Neural Network, Korea Brain Research Institute, 61 Cheomdan-ro, Dong-gu, Daegu 701-300, Korea. Chong,Y H. Division of Molecular Biology and Neuroscience, Department of Microbiology, School of Medicine, Ewha Medical Research Institute, Ewha Womans University, Seoul, 158-710, Korea. Chang,K-A. Department of Pharmacology, College of Medicine, Neuroscience Research Institute, Gachon University, Incheon, 405-760, Korea. Suh,Y-H. 1] Department of Pharmacology, College of Medicine, Neuroscience Research Institute, MRC, Seoul National University, Seoul, 110-799, Korea [2] Synaptic Circuit Plasticity Laboratory, Department of Structure & Function of Neural Network, Korea Brain Research Institute, 61 Cheomdan-ro, Dong-gu, Daegu 701-300, Korea. TI - Neural stem cell transplantation at critical period improves learning and memory through restoring synaptic impairment in Alzheimer's disease mouse model. SO - Cell Death & Disease. 6:e1789, 2015. AS - Cell Death Dis. 6:e1789, 2015. NJ - Cell death & disease AB - Alzheimer's disease (AD) is characterized by neuronal loss in several regions of the brain. Recent studies have suggested that stem cell transplantation could serve as a potential therapeutic strategy to halt or ameliorate the inexorable disease progression. However, the optimal stage of the disease for stem cell transplantation to have a therapeutic effect has yet to be determined. Here, we demonstrated that transplantation of neural stem cells into 12-month-old Tg2576 brains markedly improved both cognitive impairments and neuropathological features by reducing beta-amyloid processing and upregulating clearance of beta-amyloid, secretion of anti-inflammatory cytokines, endogenous neurogenesis, as well as synapse formation. In contrast, the stem cell transplantation did not recover cognitive dysfunction and beta-amyloid neuropathology in Tg2576 mice aged 15 months when the memory loss is manifest. Overall, this study underscores that stem cell therapy at optimal time frame is crucial to obtain maximal therapeutic effects that can restore functional deficits or stop the progression of AD. DO - http://dx.doi.org/10.1038/cddis.2015.138 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <60. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26010104 AU - Madsen C AU - Lough D AU - Lim A AU - Harshbarger RJ 3rd AU - Kumar AR FA - Madsen, Christopher FA - Lough, Denver FA - Lim, Alan FA - Harshbarger, Raymond J 3rd FA - Kumar, Anand R IN - Madsen,Christopher. *Department of Plastic and Reconstructive Surgery, The Johns Hopkins School of Medicine, Baltimore, MD +Division of Plastic and Reconstructive, Department of Surgery, Lahey Clinic, Boston, MA ++Craniofacial and Pediatric Plastic Surgery, University of Texas, Austin Medical School, Austin, TX. TI - Cleft and Craniofacial Care During Military Pediatric Plastic Surgery Humanitarian Missions. SO - Journal of Craniofacial Surgery. 26(4):1097-101, 2015 Jun. AS - J Craniofac Surg. 26(4):1097-101, 2015 Jun. NJ - The Journal of craniofacial surgery LM - Available online from MWHC library: 2001 - present, Available in print through MWHC library:1999-2007 AB - BACKGROUND: Military pediatric plastic surgery humanitarian missions in the Western Hemisphere have been initiated and developed since the early 1990 s using the Medical Readiness Education and Training Exercise (MEDRETE) concept. Despite its initial training mission status, the MEDRETE has developed into the most common and advanced low level medical mission platform currently in use. The objective of this study is to report cleft- and craniofacial-related patient outcomes after initiation and evolution of a standardized treatment protocol highlighting lessons learned which apply to civilian plastic surgery missions. AB - METHODS: A review of the MEDRETE database for pediatric plastic surgery/cleft and craniofacial missions to the Dominican Republic from 2005 to 2009 was performed. A multidisciplinary team including a craniofacial surgeon evaluated all patients with a cleft/craniofacial and/or pediatric plastic condition. A standardized mission time line included predeployment site survey and predeployment checklist, operational brief, and postdeployment after action report. Deployment data collection, remote patient follow-up, and coordination with larger land/amphibious military operations was used to increase patient follow-up data. Data collected included sex, age, diagnosis, date and type of procedure, surgical outcomes including speech scores, surgical morbidity, and mortality. AB - RESULTS: Five hundred ninety-four patients with cleft/craniofacial abnormalities were screened by a multidisciplinary team including craniofacial surgeons over 4 years. Two hundred twenty-three patients underwent 330 surgical procedures (cleft lip, 53; cleft palate, 73; revision cleft lip/nose, 73; rhinoplasty, 15; speech surgery, 24; orthognathic/distraction, 21; general pediatric plastic surgery, 58; fistula repair, 12). Average follow-up was 30 months (range, 1-60). The complication rate was 6% (n = 13) (palate fistula, lip revision, dental/alveolar loss, revision speech surgery rate). The average pre-surgical (Pittsburgh Weighted Speech Score) speech score was 12 (range, 6-24). The average postsurgical speech score was 6 (range, 0-21). Average hospital stay was 3 days for cleft surgery. There were no major complications or mortality, 1 reoperation for bleeding or infection, and 12 patients required secondary operations for palatal fistula, unsatisfactory aesthetic result, malocclusion, or velopharygeal dysfunction. AB - CONCLUSIONS: Military pediatric plastic surgery humanitarian missions can be executed with similar home institution results after the initiation and evolution of a standardized approach to humanitarian missions. The incorporation of a dedicated logistics support unit, a dedicated operational specialist (senior noncommissioned officer), a speech language pathologist, remote internet follow up, an liaison officer (host nation liaison physician participation), host nation surgical resident participation, and support from the embassy, Military Advisory Attachment Group, and United States Aid and International Development facilitated patient accurate patient evaluation and posttreatment follow-up. Movement of the mission site from a remote more austere environment to a centralized better equipped facility with host nation support to transport patients to the site facilitated improved patient safety and outcomes despite increasing the complexity of surgery performed. IL - 1049-2275 DO - http://dx.doi.org/10.1097/SCS.0000000000001686 PT - Journal Article LG - English <61. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26084340 AU - Dawkins RC AU - Oliver GF AU - Sharma M AU - Pinto BM AU - Jeronimo B AU - Pereira B AU - Magno J AU - Motta LA AU - Verma N AU - Shephard M FA - Dawkins, Rosie Claire Hewitt FA - Oliver, Genevieve Frances FA - Sharma, Manoj FA - Pinto, Basilio Martins FA - Jeronimo, Belmerio FA - Pereira, Bernadete FA - Magno, Julia FA - Motta, Lara Alexandra FA - Verma, Nitin FA - Shephard, Mark IN - Dawkins,Rosie Claire Hewitt. Sentru Matan Nasional, Hospital Nacional Guido Valadares, Bidau, Dili, Timor-Leste. rosie.dawkins@post.harvard.edu. Dawkins,Rosie Claire Hewitt. Timor-Leste Eye Program, Royal Australasian College of Surgeons, Melbourne, Australia. rosie.dawkins@post.harvard.edu. Oliver,Genevieve Frances. Sentru Matan Nasional, Hospital Nacional Guido Valadares, Bidau, Dili, Timor-Leste. eyeota@gmail.com. Oliver,Genevieve Frances. Timor-Leste Eye Program, Royal Australasian College of Surgeons, Melbourne, Australia. eyeota@gmail.com. Oliver,Genevieve Frances. Fred Hollows Foundation of New Zealand, Auckland, New Zealand. eyeota@gmail.com. Sharma,Manoj. Sentru Matan Nasional, Hospital Nacional Guido Valadares, Bidau, Dili, Timor-Leste. mansuyu@gmail.com. Sharma,Manoj. Timor-Leste Eye Program, Royal Australasian College of Surgeons, Melbourne, Australia. mansuyu@gmail.com. Pinto,Basilio Martins. Sentru Matan Nasional, Hospital Nacional Guido Valadares, Bidau, Dili, Timor-Leste. bpinto@hollows.org.nz. Pinto,Basilio Martins. Fred Hollows Foundation of New Zealand, Auckland, New Zealand. bpinto@hollows.org.nz. Jeronimo,Belmerio. Sentru Matan Nasional, Hospital Nacional Guido Valadares, Bidau, Dili, Timor-Leste. belmerio@hollows.org.nz. Jeronimo,Belmerio. Fred Hollows Foundation of New Zealand, Auckland, New Zealand. belmerio@hollows.org.nz. Pereira,Bernadete. Sentru Matan Nasional, Hospital Nacional Guido Valadares, Bidau, Dili, Timor-Leste. devio_612@yahoo.com. Magno,Julia. Sentru Matan Nasional, Hospital Nacional Guido Valadares, Bidau, Dili, Timor-Leste. suzy.magno74@gmail.com. Motta,Lara Alexandra. Flinders University International Centre for Point-of-Care Testing, Flinders University, Adelaide, Australia. lara.motta@flinders.edu.au. Verma,Nitin. Timor-Leste Eye Program, Royal Australasian College of Surgeons, Melbourne, Australia. nitver3@gmail.com. Verma,Nitin. Department of Ophthalmology, University of Tasmania, Hobart, Australia. nitver3@gmail.com. Verma,Nitin. School of Medicine, Sydney University, Sydney, Australia. nitver3@gmail.com. Shephard,Mark. Flinders University International Centre for Point-of-Care Testing, Flinders University, Adelaide, Australia. mark.shephard@flinders.edu.au. TI - An estimation of the prevalence of diabetes mellitus and diabetic retinopathy in adults in Timor-Leste. SO - BMC Research Notes. 8:249, 2015. AS - BMC Res Notes. 8:249, 2015. NJ - BMC research notes AB - BACKGROUND: Once considered an affliction of people in high-income countries, diabetes mellitus is increasingly seen as a global epidemic. However, for many countries very little is known about the prevalence of diabetes and its complications. This study aims to estimate the prevalence of diabetes, and diabetic retinopathy, in adults in Timor-Leste. AB - METHODS: From March 2013 to May 2014, adult patients being assessed for cataract surgery at the Sentru Matan Nasional (National Eye Centre) in Dili, Timor-Leste had a point-of-care HbA1c measurement performed on the DCA Vantage device (Siemens Ltd) under a quality framework. A diagnostic cut-off of 6.5% (48 mmol/mol) HbA1c was used for diagnosis of diabetes. Ocular examination, blood pressure, demographic and general health data were also collected. Diabetic retinopathy assessment was carried out by ophthalmologists. AB - RESULTS: A total of 283 people [mean age 63.6 years (range 20-90 years)] were tested and examined during the study period. Forty-three people (15.2%) were found to have diabetes, with a mean HbA1c of 9.5% (77 mmol/mol). Of these, 27 (62.9%) were newly diagnosed, with a mean HbA1c of 9.7% (83 mmol/mol) and a range of 6.6-14% (49-130 mmol/mol). Nearly half (48.1%) of people newly diagnosed with diabetes had an HbA1c over 10.0% (86 mmol/mol). Of those with known diabetes, only 68.8% were receiving any treatment. Mean HbA1c for treated patients was 9.9% (85 mmol/mol). Diabetic retinopathy was identified in 18.6% of people with diabetes, of whom half had no previous diagnosis of diabetes. AB - CONCLUSIONS: This study estimates the prevalence of diabetes at 15% in adults in Timor-Leste, a substantial proportion of whom have evidence of diabetic retinopathy. This is consistent with regional estimates. With the majority of patients undiagnosed, and management of people known to have diabetes largely inadequate, point-of-care testing is a valuable tool to assist with diabetes case detection and management. Whilst only a preliminary estimate, our data provides important impetus for further investigation of the prevalence and impact of diabetes in Timor-Leste. It provides guidance that further investment is required in expanding testing, as well as in prevention and treatment. IL - 1756-0500 DO - http://dx.doi.org/10.1186/s13104-015-1171-3 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <62. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26080193 AU - Collins JB AU - Czerwinski M FA - Collins, James B FA - Czerwinski, Marcin IN - Collins,James B. Division of Plastic Surgery, Department of Surgery, Baylor Scott & White, Temple, TX. TI - Contralateral Dorsally Based Septal Mucoperichondrial Page Flap, for Nasal Lining Reconstruction. SO - Journal of Craniofacial Surgery. 26(4):1352-4, 2015 Jun. AS - J Craniofac Surg. 26(4):1352-4, 2015 Jun. NJ - The Journal of craniofacial surgery LM - Available online from MWHC library: 2001 - present, Available in print through MWHC library:1999-2007 AB - BACKGROUND: Although excellent techniques for reconstruction of nasal cover and support have been described, reconstruction of large nasal lining defects remains a challenge. Currently available methods have several shortcomings including limited size, airway obstruction, need for multiple procedures, and creation of septal fistulae. AB - METHODS: We present 2 cases of nasal lining reconstruction for the lower and mid nasal vaults using a contralateral dorsally based septal mucoperichondrial page flap transposed dorsal to nasal septum and superficial to the ipsilateral upper lateral cartilage. Appropriate, uncomplicated, reconstruction of nasal lining was confirmed in both cases. AB - DISCUSSION: In the lower vault, the flap permits a single-stage reconstruction, without obstruction of the external nasal valve or compromise of caudal septal support. In the mid-vault, the flap allows for reconstruction without creation of a septal fistula or narrowing of the internal nasal valve. In both locations, the size of the flap may be increased by extending it onto nasal floor, and support may be added by combining the flap with septal cartilage. AB - CONCLUSION: The contralateral dorsally based septal mucoperichondrial flap is a useful option for reconstruction of lower and mid nasal vault lining defects. IL - 1049-2275 DO - http://dx.doi.org/10.1097/SCS.0000000000001732 PT - Case Reports PT - Journal Article LG - English <63. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26068791 AU - Slatter TL AU - Hung N AU - Bowie S AU - Campbell H AU - Rubio C AU - Speidel D AU - Wilson M AU - Baird M AU - Royds JA AU - Braithwaite AW FA - Slatter, T L FA - Hung, N FA - Bowie, S FA - Campbell, H FA - Rubio, C FA - Speidel, D FA - Wilson, M FA - Baird, M FA - Royds, J A FA - Braithwaite, A W IN - Slatter,T L. Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand. Hung,N. Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand. Bowie,S. Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand. Campbell,H. Children's Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia. Rubio,C. Children's Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia. Speidel,D. Children's Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia. Wilson,M. Department of Microbiology and Immunology, School of Medical Sciences, University of Otago, Dunedin, New Zealand. Baird,M. Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand. Royds,J A. Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand. Braithwaite,A W. 1] Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand [2] Children's Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia [3] Maurice Wilkins Centre for BioDiscovery, University of Otago, Dunedin, New Zealand. TI - DELTA122p53, a mouse model of DELTA133p53alpha, enhances the tumor-suppressor activities of an attenuated p53 mutant. SO - Cell Death & Disease. 6:e1783, 2015. AS - Cell Death Dis. 6:e1783, 2015. NJ - Cell death & disease AB - Growing evidence suggests the DELTA133p53alpha isoform may function as an oncogene. It is overexpressed in many tumors, stimulates pathways involved in tumor progression, and inhibits some activities of wild-type p53, including transactivation and apoptosis. We hypothesized that DELTA133p53alpha would have an even more profound effect on p53 variants with weaker tumor-suppressor capability. We tested this using a mouse model heterozygous for a DELTA133p53alpha-like isoform (DELTA122p53) and a p53 mutant with weak tumor-suppressor function (mDELTApro). The DELTA122p53/mDELTApro mice showed a unique survival curve with a wide range of survival times (92-495 days) which was much greater than mDELTApro/- mice (range 120-250 days) and mice heterozygous for the DELTA122p53 and p53 null alleles (DELTA122p53/-, range 78-150 days), suggesting DELTA122p53 increased the tumor-suppressor activity of mDELTApro. Moreover, some of the mice that survived longest only developed benign tumors. In vitro analyses to investigate why some DELTA122p53/mDELTApro mice were protected from aggressive tumors revealed that DELTA122p53 stabilized mDELTApro and prolonged the response to DNA damage. Similar effects of DELTA122p53 and DELTA133p53alpha were observed on wild-type of full-length p53, but these did not result in improved biological responses. The data suggest that DELTA122p53 (and DELTA133p53alpha) could offer some protection against tumors by enhancing the p53 response to stress. DO - http://dx.doi.org/10.1038/cddis.2015.149 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <64. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26054529 AU - Bishop L AU - Young S AU - Twells L AU - Dillon C AU - Hawboldt J FA - Bishop, Lisa FA - Young, Stephanie FA - Twells, Laurie FA - Dillon, Carla FA - Hawboldt, John IN - Bishop,Lisa. School of Pharmacy, Memorial University, 300 Prince Philip Drive, St. John's, NL, A1B 3V6, Canada. ldbishop@mun.ca. Young,Stephanie. School of Pharmacy, Memorial University, 300 Prince Philip Drive, St. John's, NL, A1B 3V6, Canada. swyoung@mun.ca. Twells,Laurie. School of Pharmacy, Memorial University, 300 Prince Philip Drive, St. John's, NL, A1B 3V6, Canada. ltwells@mun.ca. Twells,Laurie. Faculty of Medicine, Memorial University, 300 Prince Philip Drive, St. John's, NL, A1B 3V6, Canada. ltwells@mun.ca. Dillon,Carla. School of Pharmacy, Memorial University, 300 Prince Philip Drive, St. John's, NL, A1B 3V6, Canada. cmdillon@mun.ca. Hawboldt,John. School of Pharmacy, Memorial University, 300 Prince Philip Drive, St. John's, NL, A1B 3V6, Canada. hawboldt@mun.ca. TI - Patients' and physicians' satisfaction with a pharmacist managed anticoagulation program in a family medicine clinic. SO - BMC Research Notes. 8:233, 2015. AS - BMC Res Notes. 8:233, 2015. NJ - BMC research notes AB - BACKGROUND: A pharmacist managed anticoagulation service was initiated in a multi-physician family medicine clinic in December 2006. In order to determine the patient and physician satisfaction with the service, a study was designed to describe the patients' satisfaction with the warfarin education and management they received from the pharmacist, and to describe the physicians' satisfaction with the level of care provided by the pharmacist for patients taking warfarin. A self-administered survey was completed by both eligible patients receiving warfarin and physicians prescribing warfarin between December 2006 and May 2008. The patient survey collected information on patient demographics, satisfaction with warfarin education and daily warfarin management. The physician survey collected data about the satisfaction with patient education and daily anticoagulation management by the pharmacist. AB - RESULTS: Seventy-six of 94 (81%) patients completed the survey. Fifty-nine percent were male with a mean age of 65 years (range 24-90). Ninety-six percent agreed/strongly agreed the pharmacist did a good job teaching the importance of warfarin adherence, the necessity of INR testing and the risks of bleeding. Eighty-five percent agreed/strongly agreed the risk of blood clots was well explained, 79% felt the pharmacist did a good job teaching about dietary considerations and 77% agreed/strongly agreed the pharmacist explained when to see a doctor. All patients felt the pharmacist gave clear instructions on warfarin dosing and INR testing. Four of nine physicians (44%) completed the survey. All agreed/strongly agreed the pharmacist was competent in the care provided, were confident in the care their patients received, would like the pharmacist to continue the service, and would recommend this program to other clinics. AB - CONCLUSIONS: Patients and family physicians were satisfied with the pharmacist managed anticoagulation program and recommended continuation of the program. These results support the role of the pharmacist in the management of anticoagulation in a multi-physician family medicine clinic. IL - 1756-0500 DO - http://dx.doi.org/10.1186/s13104-015-1187-8 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <65. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25769506 AU - Barsky MM AU - Tucker MA AU - Stickgold R FA - Barsky, Murray M FA - Tucker, Matthew A FA - Stickgold, Robert IN - Barsky,Murray M. Center for Sleep and Cognition, Dept. of Psychiatry, Harvard Medical School, Beth Israel Deaconess Medical Center, 330 Brookline Ave/FD 861, Boston, MA 02215, USA. Electronic address: barsky@fas.harvard.edu. Tucker,Matthew A. Center for Sleep and Cognition, Dept. of Psychiatry, Harvard Medical School, Beth Israel Deaconess Medical Center, 330 Brookline Ave/FD 861, Boston, MA 02215, USA; Department of Biomedical Sciences, University of South Carolina, School of Medicine, Greenville, Health Sciences Administration Building - Rm 248, 701 Grove Road, Greenville, SC 29605, USA. Stickgold,Robert. Center for Sleep and Cognition, Dept. of Psychiatry, Harvard Medical School, Beth Israel Deaconess Medical Center, 330 Brookline Ave/FD 861, Boston, MA 02215, USA. TI - REM sleep enhancement of probabilistic classification learning is sensitive to subsequent interference. SO - Neurobiology of Learning & Memory. 122:63-8, 2015 Jul. AS - Neurobiol Learn Mem. 122:63-8, 2015 Jul. NJ - Neurobiology of learning and memory AB - During wakefulness the brain creates meaningful relationships between disparate stimuli in ways that escape conscious awareness. Processes active during sleep can strengthen these relationships, leading to more adaptive use of those stimuli when encountered during subsequent wake. Performance on the Weather Prediction Task (WPT), a well-studied measure of implicit probabilistic learning, has been shown to improve significantly following a night of sleep, with stronger initial learning predicting more nocturnal REM sleep. We investigated this relationship further, studying the effect on WPT performance of a daytime nap containing REM sleep. We also added an interference condition after the nap/wake period as an additional probe of memory strength. Our results show that a nap significantly boosts WPT performance, and that this improvement is correlated with the amount of REM sleep obtained during the nap. When interference training is introduced following the nap, however, this REM-sleep benefit vanishes. In contrast, following an equal period of wake, performance is both unchanged from training and unaffected by interference training. Thus, while the true probabilistic relationships between WPT stimuli are strengthened by sleep, these changes are selectively susceptible to the destructive effects of retroactive interference, at least in the short term. Copyright © 2015 Elsevier Inc. All rights reserved. IL - 1074-7427 DO - http://dx.doi.org/10.1016/j.nlm.2015.02.015 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English <66. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26038359 AU - Su C AU - Jia X AU - Wang Z AU - Wang H AU - Zhang B FA - Su, Chang FA - Jia, Xiaofang FA - Wang, Zhihong FA - Wang, Huijun FA - Zhang, Bing IN - Su,Chang. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China. Jia,Xiaofang. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China. Wang,Zhihong. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China. Wang,Huijun. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China. Zhang,Bing. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China. TI - Trends in dietary cholesterol intake among Chinese adults: a longitudinal study from the China Health and Nutrition Survey, 1991-2011. SO - BMJ Open. 5(6):e007532, 2015. AS - BMJ Open. 5(6):e007532, 2015. NJ - BMJ open AB - OBJECTIVES: Dietary cholesterol is the leading risk factor for cardiovascular disease and other chronic diseases. Changes in dietary patterns in China recently might have an impact on the trends of diet-related risk factors of chronic diseases. This study aims to monitor the changes in daily cholesterol intake and its food sources in Chinese adults. AB - DESIGN: A longitudinal study using demographic and dietary data of adults younger than 60 years from eight waves (1991-2011) of the China Health and Nutrition Surveys was conducted. Mixed-effect models were used in this study. AB - SETTING: The data were derived from urban and rural communities in nine provinces (autonomous regions) in China. AB - PARTICIPANTS: There were 21,273 participants (10,091 males and 11,182 females) in this study. AB - OUTCOMES: The major outcome is daily cholesterol intake amount, which was calculated by using the Chinese Food Composition Table, based on dietary data. AB - RESULTS: The mean daily cholesterol intake in Chinese adults increased from 165.8 mg/day in 1991 to 266.3 mg/day in 2011. Cholesterol consumed by participants in different age (18-39 and 40-59 years), sex and urbanisation groups steadily elevated over time (p<0.0001), as did the proportions of participants with greater than 300 mg/day cholesterol consumption. In each subgroup, cholesterol originating from most of the food groups showed increasing trends over time (p<0.0001), except for animal fat and organ meats. Eggs, pork, fish and shellfish in that order remained the top three sources in 1991, 2000 and 2011, whereas milks were a negligible contributor. Cholesterol from animal fat declined and was insignificant in 2011 in most of the subgroups, while cholesterol being of poultry origin increased and became considerable in 2011. AB - CONCLUSIONS: Adults in China consumed increasingly high cholesterol and deviated from the recommended intake level over the past two decades. Adults need to pay more attention to intakes of eggs, pork, fish and shellfish.Copyright Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions. DO - http://dx.doi.org/10.1136/bmjopen-2014-007532 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English <67. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 23416881 AU - Khan RA AU - Khan MR AU - Sahreen S AU - Ahmed M AU - Shah NA FA - Khan, Rahmat Ali FA - Khan, Muhammad Rashid FA - Sahreen, Sumaira FA - Ahmed, Mushtaq FA - Shah, Naseer Ali IN - Khan,Rahmat Ali. Faculty of Biological Sciences, Department of Biotechnology, University of Science and Technology Bannu, Khyber Pakhtunkhwa, Pakistan Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan rahmatgul_81@yahoo.com. Khan,Muhammad Rashid. Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan. Sahreen,Sumaira. Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan. Ahmed,Mushtaq. Faculty of Biological Sciences, Department of Biotechnology, University of Science and Technology Bannu, Khyber Pakhtunkhwa, Pakistan. Shah,Naseer Ali. Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-Azam University Islamabad, Pakistan. TI - Carbon tetrachloride-induced lipid peroxidation and hyperglycemia in rat: a novel study. SO - Toxicology & Industrial Health. 31(6):546-53, 2015 Jun. AS - Toxicol Ind Health. 31(6):546-53, 2015 Jun. NJ - Toxicology and industrial health AB - Launaea procumbens methanol extract (LPME) was evaluated against carbon tetrachloride (CCl4)-induced pancreatic oxidative damage and hyperglycemia in rats. Various doses of the extract were administered to rats after 48 h of CCl4 treatment (3 ml/kg bodyweight (bw); intraperitoneally, 20% CCl4/olive oil) twice a week for 4 weeks. Coadministration of various concentrations of LPME (100, 150 and 200 mg/kg) ameliorated the toxicity of CCl4 and reversed the serum level of enzymes (amylase and lipase), glucose and hormone (insulin). The extract was able to reduce thiobarbituric acid reactive substance but increased the glutathione contents in pancreatic tissue. Depletion of antioxidant enzyme activities (catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase and glutathione-S-reductase) and DNA damages induced with CCl4 were restored by LPME supplement. It is suggested that LPME effectively protects the liver against the CCl4-induced oxidative damage in rats, possibly through antioxidant and/or free radical scavenging effects of flavonoids and phenolic compounds in the extract. Copyright © The Author(s) 2013. IL - 0748-2337 DO - http://dx.doi.org/10.1177/0748233713475503 PT - Journal Article LG - English <68. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25774014 AU - Chang R AU - Petersen JR AU - Niswander LA AU - Liu A FA - Chang, Rachel FA - Petersen, Juliette R FA - Niswander, Lee A FA - Liu, Aimin IN - Chang,Rachel. Department of Biology, Eberly College of Science, The Pennsylvania State University, University Park, Pennsylvania. Petersen,Juliette R. Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado. Niswander,Lee A. Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado. Liu,Aimin. Department of Biology, Eberly College of Science, The Pennsylvania State University, University Park, Pennsylvania. Liu,Aimin. Center for Cellular Dynamics, Huck Institute of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania. TI - A hypomorphic allele reveals an important role of inturned in mouse skeletal development. SO - Developmental Dynamics. 244(6):736-47, 2015 Jun. AS - Dev Dyn. 244(6):736-47, 2015 Jun. NJ - Developmental dynamics : an official publication of the American Association of Anatomists AB - BACKGROUND: Cilia are important for Hedgehog signaling in vertebrates and many genes that encode proteins involved in ciliogenesis have been studied for their roles in embryonic development. Null mutations in many of these genes cause early embryonic lethality, hence an understanding of their roles in postnatal development is limited. AB - RESULTS: The Inturned (Intu) gene is required for ciliogenesis and here we report a recessive hypomorphic mutation, resulting in substitution of a conserved hydrophobic residue (I813N) near the C-terminus, that sheds light on later functions of Intu. Mice homozygous for this Double-thumb (Intu(Dtm)) allele exhibit polydactyly, retarded growth, and reduced survival. There is a moderate loss of cilia in Intu(Dtm/Dtm) mutants, and Intu(I813N) exhibits compromised ability to increase ciliogenesis in cultured Intu null mutant cells. Intu(Dtm) mutants show rib defects and delay of endochondral ossification in long bones, digits, vertebrae, and the sternum. These skeletal defects correlate with a decrease in Hh signaling. However, patterning of the neural tube and planar cell polarity appear to be normal. AB - CONCLUSIONS: This hypomorphic Intu allele highlights an important role of Intu in mouse skeletal development.Copyright © 2015 Wiley Periodicals, Inc. IL - 1058-8388 DO - http://dx.doi.org/10.1002/dvdy.24272 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <69. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26018733 AU - Park B AU - Lee J AU - Moon H AU - Lee G AU - Lee DH AU - Cho JH AU - Park D FA - Park, B FA - Lee, J FA - Moon, H FA - Lee, G FA - Lee, D-H FA - Cho, J Hoon FA - Park, D IN - Park,B. 1] School of Life Sciences and Bio Imaging Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea [2] Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea. Lee,J. 1] School of Life Sciences and Bio Imaging Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea [2] Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea. Moon,H. 1] School of Life Sciences and Bio Imaging Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea [2] Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea. Lee,G. School of Life Sciences and Bio Imaging Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea. Lee,D-H. Department of Surgery and Pharmacology and Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA. Cho,J Hoon. Department of Biology Education, College of Education, Chosun University, Gwangju 501-759, Korea. Park,D. 1] School of Life Sciences and Bio Imaging Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea [2] Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea. TI - Co-receptors are dispensable for tethering receptor-mediated phagocytosis of apoptotic cells. SO - Cell Death & Disease. 6:e1772, 2015. AS - Cell Death Dis. 6:e1772, 2015. NJ - Cell death & disease AB - During efferocytosis, phagocytic cells recognize dying cells by receptors binding to ligands specifically exposed on apoptotic cells. Multiple phagocytic receptors and some of their signaling pathways have been identified. However, the downstream pathways of tethering receptors that secure apoptotic cells remain elusive. It is generally assumed that tethering receptors induce signaling to mediate engulfment via interacting with co-receptors or other engulfment receptors located nearby. However, it is poorly understood whether co-receptors for tethering receptors exist during efferocytosis, and, if they do, whether they are indispensable for this process. Here, we address this issue using glycophosphatidylinositol (GPI)-anchored annexin A5 (Anxa5-GPI), an artificial tethering receptor without a putative co-receptor. Phagocytes expressing Anxa5-GPI exhibited enhanced binding of apoptotic cells, resulting in promoted ingestion of apoptotic cells in a phosphatidylserine-dependent manner. Anxa5-GPI-induced phagocytosis of apoptotic cells relied on the known cytoskeletal engulfment machinery but partially depended on the Elmo-Dock-Rac module or the integrin pathway. In addition, Anxa5-GPI-mediated efferocytosis provoked anti-inflammatory responses. Taken together, our work suggests that co-receptors are dispensable for tethering receptor-induced efferocytosis and that tethering receptors mediate the engulfment of apoptotic cells through multiple engulfment signaling pathways. DO - http://dx.doi.org/10.1038/cddis.2015.140 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <70. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25777970 AU - Shin H AU - Ryu HH AU - Kwon O AU - Park BS AU - Jo SJ FA - Shin, Hyoseung FA - Ryu, Hyeong Ho FA - Kwon, Ohsang FA - Park, Byung-Soon FA - Jo, Seong Jin IN - Shin,Hyoseung. Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, South Korea. Ryu,Hyeong Ho. Department of Dermatology, Seoul National University College of Medicine, Seoul, South Korea. Kwon,Ohsang. Department of Dermatology, Seoul National University College of Medicine, Seoul, South Korea. Park,Byung-Soon. Cellpark Clinic, Seoul, South Korea. Jo,Seong Jin. Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, South Korea. TI - Clinical use of conditioned media of adipose tissue-derived stem cells in female pattern hair loss: a retrospective case series study. SO - International Journal of Dermatology. 54(6):730-5, 2015 Jun. AS - Int J Dermatol. 54(6):730-5, 2015 Jun. NJ - International journal of dermatology LM - Available online through MWHC library: 2011 only, Available in print through MWHC library: 1999 - 2006 AB - BACKGROUND: Female pattern hair loss (FPHL) is a common disorder but presents severe psychosocial problems in many female patients. Adipose tissue-derived stem cells (ADSCs) and conditioned media of ADSCs (ADSC-CM) are reported to promote hair growth in vitro. However, there are no clinical reports on the treatment of alopecia using ADSC-CM. AB - OBJECTIVES: This study evaluates our clinical experience in the use of ADSC-CM for the treatment of FPHL. AB - METHODS: A retrospective, observational study of outcomes in 27 patients with FPHL treated with ADSC-CM was performed. To evaluate the efficacy of the treatment, patients' medical records and phototrichographic images were analyzed. AB - RESULTS: The application of ADSC-CM showed efficacy in treating FPHL after 12 weeks of therapy. Hair density increased from 105.4 to 122.7 hairs/cm(2) (P < 0.001). Hair thickness increased from 57.5 mum to 64.0 mum (P < 0.001). None of the patients reported severe adverse reactions. AB - CONCLUSIONS: The application of ADSC-CM is a potential treatment option for FPHL.Copyright © 2015 The International Society of Dermatology. IL - 0011-9059 DO - http://dx.doi.org/10.1111/ijd.12650 PT - Clinical Study PT - Journal Article LG - English <71. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 23786248 AU - Yong AS AU - Murphy JG AU - Shah N FA - Yong, Adrian S W FA - Murphy, Joseph G FA - Shah, Nasir IN - Yong,Adrian S W. Department of Dermatology, Norfolk and Norwich University Hospital, Norwich, UK. Murphy,Joseph G. Department of Cellular Pathology, Norfolk and Norwich University Hospital, Norwich, UK. Shah,Nasir. Department of Dermatology, Norfolk and Norwich University Hospital, Norwich, UK. TI - Nodular localized cutaneous amyloidosis in an immunosuppressed patient. SO - International Journal of Dermatology. 54(6):708-9, 2015 Jun. AS - Int J Dermatol. 54(6):708-9, 2015 Jun. NJ - International journal of dermatology LM - Available online through MWHC library: 2011 only, Available in print through MWHC library: 1999 - 2006 IL - 0011-9059 DO - http://dx.doi.org/10.1111/ijd.12140 PT - Case Reports PT - Journal Article LG - English <72. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 23786159 AU - Su Z AU - Lu Y AU - Ge Y AU - Jiang J AU - Jia Z AU - Zhu F AU - Zhang M AU - Ji C AU - Tang Y AU - Wei J AU - Gao Q AU - Wang H FA - Su, Zhonglan FA - Lu, Yan FA - Ge, Yixin FA - Jiang, Jieyao FA - Jia, Zhenyu FA - Zhu, Feng FA - Zhang, Meihua FA - Ji, Chao FA - Tang, Yicheng FA - Wei, Jiwu FA - Gao, Qian FA - Wang, Hongwei IN - Su,Zhonglan. Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing Universityg, Nanjing, China. Su,Zhonglan. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Lu,Yan. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Ge,Yixin. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Jiang,Jieyao. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Jia,Zhenyu. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Zhu,Feng. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Zhang,Meihua. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Ji,Chao. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Tang,Yicheng. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Wei,Jiwu. Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing Universityg, Nanjing, China. Gao,Qian. Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing Universityg, Nanjing, China. Wang,Hongwei. Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing Universityg, Nanjing, China. TI - Central nervous system involvement in systemic malignant atrophic papulosis (Degos disease): a case report. SO - International Journal of Dermatology. 54(6):699-703, 2015 Jun. AS - Int J Dermatol. 54(6):699-703, 2015 Jun. NJ - International journal of dermatology LM - Available online through MWHC library: 2011 only, Available in print through MWHC library: 1999 - 2006 IL - 0011-9059 DO - http://dx.doi.org/10.1111/ijd.12123 PT - Case Reports PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <73. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 26009948 AU - Morgan H AU - McLean K AU - Chapman C AU - Fitzgerald J AU - Yousuf A AU - Hammoud M FA - Morgan, Helen FA - McLean, Karen FA - Chapman, Chris FA - Fitzgerald, James FA - Yousuf, Aisha FA - Hammoud, Maya IN - Morgan,Helen. Departments of Obstetrics and Gynecology and Learning Health Sciences, University of Michigan Medical School, Ann Arbor, Michigan, USA. McLean,Karen. Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Michigan Medical School, Ann Arbor, Michigan, USA. Chapman,Chris. Instructional Design & Technology Team, University of Michigan Medical School, Ann Arbor, Michigan, USA. Fitzgerald,James. Department of Learning Health Science, University of Michigan Medical School, Ann Arbor, Michigan, USA. Yousuf,Aisha. Department of Obstetrics & Gynecology, Sidra Medical and Research Centre, Doha, Qatar. Hammoud,Maya. Departments of Obstetrics and Gynecology and Learning Health Sciences, University of Michigan Medical School, Ann Arbor, Michigan, USA. TI - The flipped classroom for medical students. SO - The clinical teacher. 12(3):155-60, 2015 Jun. AS - Clin Teach. 12(3):155-60, 2015 Jun. NJ - The clinical teacher AB - BACKGROUND: The objectives of this curricular innovation project were to implement a flipped classroom curriculum for the gynaecologic oncology topics of the obstetrics and gynaecology medical student clerkship, and to evaluate student satisfaction with the change. AB - METHODS: Four short online videos on the topics of endometrial hyperplasia, cervical dysplasia, evaluation of an adnexal mass, and ovarian cancer were created, and students were instructed to view them prior to a class-time active learning session. The Learning Activity Management System (lams) open-source online platform was used to create an active learning class-time activity that consisted of a coached discussion of cases. Student satisfaction with the two aspects of the flipped curriculum was obtained. In addition, lecture assessment for the gynaecologic oncology topics and aggregate student performance on the gynaecological oncology questions of the US National Board of Medical Examiners (NBME) Subject Examination were compared before and after implementation of the curriculum. AB - RESULTS: Eighty-nine students rotated on the clerkship during the pilot period of analysis. Seventy-one students (80%) viewed the videos prior to the class session, and 84 (94%) attended the session. Student satisfaction was very high for both parts of the curriculum. There was no significant difference in aggregate student performance on the gynaecological oncology questions of the NBME Subject Examination. The flipped classroom curriculum demonstrates a promising platform for using technology to make better use of students' time AB - DISCUSSION: Our implementation of the flipped classroom curriculum for the gynaecologic oncology topics successfully demonstrates a promising platform for using technology to make better use of our students' time, and for increasing their satisfaction with the necessary didactic learning of the clerkship.Copyright © 2015 John Wiley & Sons Ltd. IL - 1743-4971 DO - http://dx.doi.org/10.1111/tct.12328 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <74. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25903196 AU - Jia L AU - Hu C AU - Wang H AU - Liu Y AU - Liu X AU - Zhang YY AU - Li W AU - Wang LX AU - Cao YF AU - Fang ZZ FA - Jia, Lin FA - Hu, Cuimin FA - Wang, Haina FA - Liu, Yongzhe FA - Liu, Xin FA - Zhang, Yan-Yan FA - Li, Wei FA - Wang, Li-Xuan FA - Cao, Yun-Feng FA - Fang, Zhong-Ze IN - Jia,Lin. First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning, People's Republic of China. Hu,Cuimin. Tianjin Life Science Research Center and Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, People's Republic of China. Wang,Haina. College of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China. Liu,Yongzhe. Department of Toxicology, School of Public Health, Tianjin Medical University, Heping District, Tianjin, People's Republic of China. Liu,Xin. First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning, People's Republic of China. Zhang,Yan-Yan. Joint Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences and First Affiliated Hospital of Liaoning Medical University, Dalian, People's Republic of China. Li,Wei. Yangzhou University, Yangzhou, China. Wang,Li-Xuan. Joint Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences and First Affiliated Hospital of Liaoning Medical University, Dalian, People's Republic of China. Cao,Yun-Feng. First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning, People's Republic of China. Cao,Yun-Feng. Joint Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences and First Affiliated Hospital of Liaoning Medical University, Dalian, People's Republic of China. Cao,Yun-Feng. Key Laboratory of Contraceptives and Devices Research (NPFPC), Shanghai Engineer and Technology Research Center of Reproductive Health Drug and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, People's Republic of China. Fang,Zhong-Ze. Department of Toxicology, School of Public Health, Tianjin Medical University, Heping District, Tianjin, People's Republic of China. Fang,Zhong-Ze. Joint Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences and First Affiliated Hospital of Liaoning Medical University, Dalian, People's Republic of China. TI - Chirality Influence of Zaltoprofen Towards UDP-Glucuronosyltransferases (UGTs) Inhibition Potential. SO - Chirality. 27(6):359-63, 2015 Jun. AS - Chirality. 27(6):359-63, 2015 Jun. NJ - Chirality AB - Zaltoprofen (ZLT) is a nonsteroidal antiinflammation drug, and has been clinically employed to treat rheumatoid arthritis, osteoarthritis, and other chronic inflammatory pain conditions. The present study aims to investigate the chirality influence of zaltoprofen towards the inhibition potential towards UDP-glucuronosyltransferases (UGTs) isoforms. In vitro a recombinant UGT isoforms-catalyzed 4-methylumbelliferone (4-MU) glucuronidation incubation system was employed to investigate the inhibition of (R)-zaltoprofen and (S)-zaltoprofen towards UGT isoforms. The inhibition difference capability was observed for the inhibition of (R)-zaltoprofen and (S)-zaltoprofen towards UGT1A8 and UGT2B7, but not for other tested UGT isoforms. (R)-zaltoprofen exhibited noncompetitive inhibition towards UGT1A8 and competitive inhibition towards UGT2B7. The inhibition kinetic parameters were calculated to be 35.3 muM and 19.2 muM for UGT1A8 and UGT2B7. (R)-zaltoprofen and (S)-zaltoprofen exhibited a different inhibition type towards UGT1A7. Based on the reported maximum plasma concentration of (R)-zaltoprofen in vivo, a high drug-drug interaction between (R)-zaltoprofen and the drugs mainly undergoing UGT1A7, UGT1A8, and UGT2B7-catalyzed glucuronidation was indicated.Copyright © 2015 Wiley Periodicals, Inc. IL - 0899-0042 DO - http://dx.doi.org/10.1002/chir.22436 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <75. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25866238 AU - Kolber MK AU - Shukla PA AU - Kumar A AU - Silberzweig JE FA - Kolber, Marcin K FA - Shukla, Pratik A FA - Kumar, Abhishek FA - Silberzweig, James E IN - Kolber,Marcin K. Division of Vascular and Interventional Radiology, Department of Radiology, Mount Sinai Beth Israel, First Avenue at 16th Street, New York, NY 10003. Shukla,Pratik A. Division of Vascular and Interventional Radiology, Department of Radiology, Mount Sinai Beth Israel, First Avenue at 16th Street, New York, NY 10003. Kumar,Abhishek. Division of Vascular and Interventional Radiology, Department of Radiology, Mount Sinai Beth Israel, First Avenue at 16th Street, New York, NY 10003. Silberzweig,James E. Division of Vascular and Interventional Radiology, Department of Radiology, Mount Sinai Beth Israel, First Avenue at 16th Street, New York, NY 10003. Electronic address: jsilberzweig@chpnet.org. TI - Ethylene vinyl alcohol copolymer (onyx) embolization for acute hemorrhage: a systematic review of peripheral applications. [Review] SO - Journal of Vascular & Interventional Radiology. 26(6):809-15, 2015 Jun. AS - J Vasc Interv Radiol. 26(6):809-15, 2015 Jun. NJ - Journal of vascular and interventional radiology : JVIR LM - Available online through MWHC library: 2002 - 2006, Available in print through MWHC library: 1999 - 2006 AB - PURPOSE: To evaluate the endovascular use of ethylene vinyl alcohol (EVOH) copolymer (Onyx; ev3 Endovascular, Inc, Plymouth, Minnesota) for the treatment of acute peripheral nonneurologic hemorrhage. AB - MATERIALS AND METHODS: MEDLINE and PubMed databases were searched for articles published in English from 1946 to August 2014 describing patients treated for hemorrhage with EVOH copolymer outside of its usual neurovascular applications. Additional cases were collected from the bibliographies of relevant articles. Full-text articles were obtained. Demographic data, clinical presentation, underlying etiology, culprit vessel, endovascular treatment, outcomes, and follow-up times were obtained. AB - RESULTS: The literature search yielded 19 relevant articles. Cases of 131 patients (87 men, 44 women; mean age, 61 y +/- 19.2) describing the treatment of 151 lesions were reviewed. The most common categories of arterial lesions were gastrointestinal (n = 53) and bronchial (n = 40) in origin. In 14 cases, EVOH copolymer was employed after failure or rebleeding following the use of a different embolic agent. Average follow-up time was 12.0 months. EVOH copolymer was the sole embolic agent used in 105 patients. Rebleeding after treatment with EVOH copolymer occurred in 10 patients. There were 2 technical failures. One patient died of multiorgan failure in the setting of persistent hemoptysis. Complications included 2 nerve injuries and 2 minor strokes. AB - CONCLUSIONS: Embolization of acute hemorrhage in the peripheral vasculature was safe and effective with EVOH copolymer.Copyright © 2015 SIR. Published by Elsevier Inc. All rights reserved. IL - 1051-0443 DO - http://dx.doi.org/10.1016/j.jvir.2015.02.025 PT - Journal Article PT - Review LG - English <76. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25801463 AU - Gamanagatti S AU - Rangarajan K AU - Kumar A AU - Jineesh FA - Gamanagatti, Shivanand FA - Rangarajan, Krthika FA - Kumar, Atin FA - Jineesh IN - Gamanagatti,Shivanand. Department of Radiology, JPNA Trauma center, All India Institute of Medical Sciences, New Delhi, India. Electronic address: shiv223@gmail.com. Rangarajan,Krthika. Department of Radiology, JPNA Trauma center, All India Institute of Medical Sciences, New Delhi, India. Kumar,Atin. Department of Radiology, JPNA Trauma center, All India Institute of Medical Sciences, New Delhi, India. Jineesh,. Department of Radiology, JPNA Trauma center, All India Institute of Medical Sciences, New Delhi, India. TI - Blunt abdominal trauma: imaging and intervention. [Review] SO - Current Problems in Diagnostic Radiology. 44(4):321-36, 2015 Jul-Aug. AS - Curr Probl Diagn Radiol. 44(4):321-36, 2015 Jul-Aug. NJ - Current problems in diagnostic radiology AB - Interventional radiology, particularly percutaneous angioembolization, plays an important role in the management of blunt abdominal trauma involving solid organs and pelvic fractures. The traumatic injuries of the central nervous system, heart, and great vessels often lead to death at the site of trauma. Although patients with visceral organ injuries can also die at the site of trauma, these patients often reach the hospital thus giving us an opportunity to treat them with surgical or radiological intervention depending on the clinical condition of the patient. The management of these patients with trauma is now well codified-patients who remain unstable despite resuscitation should be shifted either to an operating room for laparotomy if the ultrasound (US) revealed hemoperitoneum or to a interventional room for angioembolization in cases of pelvic fractures. In all other cases, computed tomography is essential. Currently, multidetector computed tomography with contrast is the gold standard imaging modality for the diagnosis of traumatic abdominal injuries; it helps in assessing the extent of injuries, and further management can be planned. Copyright © 2015 Mosby, Inc. All rights reserved. IL - 0363-0188 DO - http://dx.doi.org/10.1067/j.cpradiol.2015.02.005 PT - Journal Article PT - Review LG - English <77. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25993655 AU - Kiniwa Y AU - Li J AU - Wang M AU - Sun C AU - Lee JE AU - Wang RF AU - Wang HY FA - Kiniwa, Yukiko FA - Li, Jiang FA - Wang, Mingjun FA - Sun, Chuang FA - Lee, Jeffrey E FA - Wang, Rong-Fu FA - Wang, Helen Y IN - Kiniwa,Yukiko. Center for Cell and Gene Therapy, Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America. Li,Jiang. Center for Cell and Gene Therapy, Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America. Wang,Mingjun. Center for Inflammation and Epigenetics, Houston Methodist Research Institute, Houston, Texas, United States of America. Sun,Chuang. Center for Inflammation and Epigenetics, Houston Methodist Research Institute, Houston, Texas, United States of America. Lee,Jeffrey E. Department of Surgical Oncology, UT MD Anderson Cancer Center, Houston, Texas, United States of America. Wang,Rong-Fu. Center for Cell and Gene Therapy, Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America; Center for Inflammation and Epigenetics, Houston Methodist Research Institute, Houston, Texas, United States of America. Wang,Helen Y. Center for Cell and Gene Therapy, Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America; Center for Inflammation and Epigenetics, Houston Methodist Research Institute, Houston, Texas, United States of America. TI - Identification of DRG-1 As a Melanoma-Associated Antigen Recognized by CD4+ Th1 Cells. SO - PLoS ONE [Electronic Resource]. 10(5):e0124094, 2015. AS - PLoS ONE. 10(5):e0124094, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - Immunotherapy has emerged as a promising strategy for the treatment of metastatic melanoma. Clinical studies have demonstrated the feasibility of cancer immunotherapy using tumor antigens recognized by CD8(+) T cells. However, the overall immune responses induced by these antigens are too weak and transient to induce tumor regression in the majority of patients who received immunization. A growing body of evidence suggests that CD4(+) T helper (Th) cells play an important role in antitumor immunity. Therefore, the identification of MHC class II-restricted tumor antigens capable of stimulating CD4(+) T cells may provide opportunities for developing effective cancer vaccines. To this end, we describe the identification of developmentally regulated GTP-binding protein 1 (DRG-1) as a melanoma-associated antigen recognized by HLA-DR11-restricted CD4(+) Th1 cells. Epitope mapping analysis showed that the DRG1248-268 epitope of DRG-1 was required for T cell recognition. Reverse transcription-polymerase chain reaction revealed that DRG-1 was highly expressed in melanoma cell lines but not in normal tissues. DRG-1 knockdown by lentiviral-based shRNA suppressed melanoma cell proliferation and soft agar colony formation. Taken together, these data suggest that DRG-1 plays an important role in melanoma cell growth and transformation, indicating that DRG1 may represent a novel target for CD4(+) T cell-mediated immunotherapy in melanoma. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0124094 PT - Journal Article PT - Research Support, N.I.H., Extramural LG - English <78. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25992614 AU - Seong JM AU - Choi NK AU - Shin JY AU - Chang Y AU - Kim YJ AU - Lee J AU - Kim JY AU - Park BJ FA - Seong, Jong-Mi FA - Choi, Nam-Kyong FA - Shin, Ju-Young FA - Chang, Yoosoo FA - Kim, Ye-Jee FA - Lee, Joongyub FA - Kim, Ju-Young FA - Park, Byung-Joo IN - Seong,Jong-Mi. Office of Drug Safety Information II, Korea Institute of Drug Safety & Risk Management, Seoul, Republic of Korea; Department of Preventive Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea. Choi,Nam-Kyong. Division of Clinical Epidemiology, Medical Research Collaborating Center, Seoul National University College of Medicine/Seoul National University Hospital, Seoul, Republic of Korea; Medical Research Center, Seoul National University, Seoul, Republic of Korea. Shin,Ju-Young. Office of Drug Utilization Review, Korea Institute of Drug Safety & Risk Management, Seoul, Republic of Korea. Chang,Yoosoo. Department of Occupational and Environmental Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University, School of Medicine, Seoul, Republic of Korea. Kim,Ye-Jee. Office of Drug Safety Information I, Korea Institute of Drug Safety & Risk Management, Seoul, Republic of Korea. Lee,Joongyub. Division of Clinical Epidemiology, Medical Research Collaborating Center, Seoul National University College of Medicine/Seoul National University Hospital, Seoul, Republic of Korea. Kim,Ju-Young. Department of Family Medicine, Seoul National University Bundang Hospital, Seoul, Republic of Korea. Park,Byung-Joo. Office of Drug Safety Information II, Korea Institute of Drug Safety & Risk Management, Seoul, Republic of Korea; Department of Preventive Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea; Office of Drug Utilization Review, Korea Institute of Drug Safety & Risk Management, Seoul, Republic of Korea; Office of Drug Safety Information I, Korea Institute of Drug Safety & Risk Management, Seoul, Republic of Korea. TI - Differential cardiovascular outcomes after dipeptidyl peptidase-4 inhibitor, sulfonylurea, and pioglitazone therapy, all in combination with metformin, for type 2 diabetes: a population-based cohort study. SO - PLoS ONE [Electronic Resource]. 10(5):e0124287, 2015. AS - PLoS ONE. 10(5):e0124287, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - BACKGROUND/OBJECTIVES: Data on the comparative effectiveness of oral antidiabetics on cardiovascular outcomes in a clinical practice setting are limited. This study sought to determine whether a differential risk of cardiovascular disease (CVD) exists for the combination of a dipeptidyl peptidase-4 (DPP-4) inhibitor plus metformin versus a sulfonylurea derivative plus metformin or pioglitazone plus metformin. AB - METHODS: We conducted a cohort study of 349,476 patients who received treatment with a DPP-4 inhibitor, sulfonylurea, or pioglitazone plus metformin for type 2 diabetes using the Korean national health insurance claims database. The incidence of total CVD and individual outcomes of myocardial infarction (MI), heart failure (HF), and ischemic stroke (IS) were assessed using the hazard ratios (HRs) estimated from a Cox proportional-hazards model weighted for a propensity score. AB - RESULTS: During follow-up, 3,881 patients developed a CVD, including 428 MIs, 212 HFs, and 1,487 ISs. The adjusted HR with 95% confidence interval (CI) for a sulfonylurea derivative plus metformin compared with a DPP-4 inhibitor plus metformin was 1.20 (1.09-1.32) for total CVD; 1.14 (1.04-1.91) for MI; 1.07 (0.71-1.62) for HF; and 1.51 (1.28-1.79) for IS. The HRs with 95% CI for total CVD, MI, HF, and IS for pioglitazone plus metformin were 0.89 (0.81-0.99), 1.05 (0.76-1.46), 4.81 (3.53-6.56), and 0.81 (0.67-0.99), respectively. AB - CONCLUSIONS: Compared with a DPP-4 inhibitor plus metformin, treatment with a sulfonylurea drug plus metformin was associated with increased risks of total CVD, MI, and IS, whereas the use of pioglitazone plus metformin was associated with decreased total CVD and IS risks. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0124287 PT - Journal Article LG - English <79. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25988386 AU - Wang Z AU - Wang J AU - Xie R AU - Liu R AU - Lu Y FA - Wang, Zhicheng FA - Wang, Jie FA - Xie, Rufeng FA - Liu, Ruilai FA - Lu, Yuan IN - Wang,Zhicheng. Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China. ahwzc@126.com. Wang,Jie. Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China. Jiewang2015@126.com. Xie,Rufeng. Blood Engineering Laboratory, Shanghai Blood Center, Shanghai 200051, China. xierufeng555@163.com. Liu,Ruilai. Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China. liuruilai88@163.com. Lu,Yuan. Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China. yuanlu@hsh.stn.sh.cn. TI - Mitochondria-derived reactive oxygen species play an important role in Doxorubicin-induced platelet apoptosis. SO - International Journal of Molecular Sciences. 16(5):11087-100, 2015. AS - Int. j. mol. sci.. 16(5):11087-100, 2015. NJ - International journal of molecular sciences AB - Doxorubicin (DOX) is an effective chemotherapeutic agent; however; its use is limited by some side effects; such as cardiotoxicity and thrombocytopenia. DOX-induced cardiotoxicity has been intensively investigated; however; DOX-induced thrombocytopenia has not been clearly elucidated. Here we show that DOX-induced mitochondria-mediated intrinsic apoptosis and glycoprotein (GP)Ibalpha shedding in platelets. DOX did not induce platelet activation; whereas; DOX obviously reduced adenosine diphosphate (ADP)- and thrombin-induced platelet aggregation; and impaired platelet adhesion on the von Willebrand factor (vWF) surface. In addition; we also show that DOX induced intracellular reactive oxygen species (ROS) production and mitochondrial ROS generation in a dose-dependent manner. The mitochondria-targeted ROS scavenger Mito-TEMPO blocked intracellular ROS and mitochondrial ROS generation. Furthermore; Mito-TEMPO reduced DOX-induced platelet apoptosis and GPIbalpha shedding. These data indicate that DOX induces platelet apoptosis; and impairs platelet function. Mitochondrial ROS play a pivotal role in DOX-induced platelet apoptosis and GPIbalpha shedding. Therefore; DOX-induced platelet apoptosis might contribute to DOX-triggered thrombocytopenia; and mitochondria-targeted ROS scavenger would have potential clinical utility in platelet-associated disorders involving mitochondrial oxidative damage. IL - 1422-0067 DO - http://dx.doi.org/10.3390/ijms160511087 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <80. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25947644 AU - Zhang R AU - Wang Y AU - Chen F AU - Wang Q AU - Wang Z AU - Chen Y AU - Xiao W FA - Zhang, Rui FA - Wang, Yao FA - Chen, Feng FA - Wang, Qidong FA - Wang, Zhaoming FA - Chen, Yilun FA - Xiao, Wenbo IN - Zhang,Rui. Department of Radiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Wang,Yao. Department of Ultrasonography, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Chen,Feng. Department of Radiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Wang,Qidong. Department of Radiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Wang,Zhaoming. Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Chen,Yilun. Kidney Disease Centre, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Xiao,Wenbo. Department of Radiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China xiaowb.111@163.com. TI - Noninvasive evaluation of renal oxygenation in primary nephrotic syndrome with blood oxygen level dependent magnetic resonance imaging: Initial experience. SO - Journal of International Medical Research. 43(3):356-63, 2015 Jun. AS - J Int Med Res. 43(3):356-63, 2015 Jun. NJ - The Journal of international medical research AB - OBJECTIVES: To use blood oxygen level dependent (BOLD) magnetic resonance imaging (MRI) to evaluate renal oxygenation in patients with primary nephrotic syndrome (PNS), and test the hypothesis that renal tissue oxygenation correlates with renal function, tubulointerstitial alterations and treatment response. AB - METHODS: Patients with untreated first-onset PNS and healthy control subjects underwent BOLD MRI. Blood and urine samples were obtained on the day of MRI, and patients underwent renal biopsy the day after MRI. Renal tubulointerstitial damage scores (TIDS) were determined using Katafuchi criteria. All patients received corticosteroids within 7 days after MRI and were followed up for 12 months. AB - RESULTS: Medullary R2* values were significantly lower in patients with PNS (n = 20) than controls (n = 18). Medullary R2* values were negatively correlated with estimated glomerular filtration rates and positively correlated with TIDS in patients with PNS. There were no significant differences in medullary or cortical R2* values when patients were classified according to treatment response. AB - CONCLUSIONS: The medullary oxygen concentration was higher in patients with PNS than in control subjects. BOLD MRI was a useful noninvasive method for the evaluation of renal function and tubulointerstitial impairment.Copyright © The Author(s) 2015 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav. IL - 0300-0605 DO - http://dx.doi.org/10.1177/0300060515579117 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <81. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25877716 AU - Jain CK AU - Arora S AU - Khanna A AU - Gupta M AU - Wadhwa G AU - Sharma SK FA - Jain, Chakresh K FA - Arora, Shivam FA - Khanna, Aparna FA - Gupta, Money FA - Wadhwa, Gulshan FA - Sharma, Sanjeev K IN - Jain,Chakresh K. Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida- 201307, Uttar Pradesh, India. ckj522@yahoo.com. TI - The ubiquitin-proteasome pathway an emerging anticancer strategy for therapeutics: a patent analysis. [Review] SO - Recent Patents on Anti-Cancer Drug Discovery. 10(2):201-13, 2015. AS - Recent Patents Anticancer Drug Discov. 10(2):201-13, 2015. NJ - Recent patents on anti-cancer drug discovery AB - The degradation of intracellular proteins is targeted by ubiquitin via non-lysosomal proteolytic pathway in the cell system. These ubiquitin molecules have been found to be conserved from yeast to humans. Ubiquitin proteasome machinery utilises ATP and other mechanisms for degrading proteins of cytosol as well as nucleus. This process of ubiquitination is regulated by activating the E3 enzyme ligase, involved in phosphorylation. In humans, proteins which regulate the cell cycle are controlled by ubiquitin; therefore the ubiquitin-proteasome pathway can be targeted for novel anti-cancer strategies. Dysregulation of the components of the ubiquitin system has been linked to many diseases like cancer and inflammation. The primary triggering mechanism (apoptosis) of these diseases can also be induced when TNF-related apoptosis-inducing ligand (TRAIL) binds to its specific receptor DR4 and DR5. In this review, the emerging prospects and importance of ubiquitin proteasome pathway as an evolving anticancer strategy have been discussed. Current challenges in the field of drug discovery have also been discussed on the basis of recent patents on cancer diagnosis and therapeutics. IL - 1574-8928 PT - Journal Article PT - Review LG - English <82. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25973977 AU - Baez S AU - Malizia A AU - Carilla J AU - Blundo C AU - Aguilar M AU - Aguirre N AU - Aquirre Z AU - Alvarez E AU - Cuesta F AU - Duque A AU - Farfan-Rios W AU - Garcia-Cabrera K AU - Grau R AU - Homeier J AU - Linares-Palomino R AU - Malizia LR AU - Cruz OM AU - Osinaga O AU - Phillips OL AU - Reynel C AU - Silman MR AU - Feeley KJ FA - Baez, Selene FA - Malizia, Agustina FA - Carilla, Julieta FA - Blundo, Cecilia FA - Aguilar, Manuel FA - Aguirre, Nikolay FA - Aquirre, Zhofre FA - Alvarez, Esteban FA - Cuesta, Francisco FA - Duque, Alvaro FA - Farfan-Rios, William FA - Garcia-Cabrera, Karina FA - Grau, Ricardo FA - Homeier, Jurgen FA - Linares-Palomino, Reynaldo FA - Malizia, Lucio R FA - Cruz, Omar Melo FA - Osinaga, Oriana FA - Phillips, Oliver L FA - Reynel, Carlos FA - Silman, Miles R FA - Feeley, Kenneth J IN - Baez,Selene. Consorcio para el Desarrollo Sostenible de la Ecoregion Andina (CONDESAN), Quito, Ecuador; Universidad Tecnica Particular de Loja, Loja, Ecuador. Malizia,Agustina. Consejo Nacional de Ciencias de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina; Instituto de Ecologia Regional (IER), Universidad Nacional de Tucuman, Tucuman, Argentina. Carilla,Julieta. Consejo Nacional de Ciencias de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina; Instituto de Ecologia Regional (IER), Universidad Nacional de Tucuman, Tucuman, Argentina. Blundo,Cecilia. Consejo Nacional de Ciencias de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina; Instituto de Ecologia Regional (IER), Universidad Nacional de Tucuman, Tucuman, Argentina. Aguilar,Manuel. Facultad de Ciencias Forestales, Universidad Nacional Agraria La Molina, Lima, Peru Aguirre,Nikolay. Universidad Nacional de Loja, Loja, Ecuador. Aquirre,Zhofre. Universidad Nacional de Loja, Loja, Ecuador. Alvarez,Esteban. Laboratorio de Servicios Ecosistemicos y Cambio Climatico, Jardin Botanico de Medellin, Medellin, Colombia. Cuesta,Francisco. Consorcio para el Desarrollo Sostenible de la Ecoregion Andina (CONDESAN), Quito, Ecuador. Duque,Alvaro. Departamento de Ciencias Forestales, Universidad Nacional de Colombia, Medellin, Colombia. Farfan-Rios,William. Department of Biology, Wake Forest University, Winston-Salem, North Carolina, United States of America. Garcia-Cabrera,Karina. Department of Biology, Wake Forest University, Winston-Salem, North Carolina, United States of America. Grau,Ricardo. Consejo Nacional de Ciencias de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina. Homeier,Jurgen. Plant Ecology, University of Gottingen, Gottingen, Germany. Linares-Palomino,Reynaldo. Facultad de Ciencias Forestales, Universidad Nacional Agraria La Molina, Lima, Peru Malizia,Lucio R. Facultad de Ciencias Agrarias, Universidad Nacional de Jujuy, Jujuy, Argentina; Fundacion ProYungas, Jujuy, Argentina. Cruz,Omar Melo. Grupo de investigacion en Biodiversidad y Dinamica de Ecosistemas Tropicales, Universidad del Tolima, Bogota, Colombia. Osinaga,Oriana. Instituto de Ecologia Regional (IER), Universidad Nacional de Tucuman, Tucuman, Argentina. Phillips,Oliver L. School of Geography, University of Leeds, Leeds, United Kingdom. Reynel,Carlos. Facultad de Ciencias Forestales, Universidad Nacional Agraria La Molina, Lima, Peru Silman,Miles R. Department of Biology, Wake Forest University, Winston-Salem, North Carolina, United States of America. Feeley,Kenneth J. International Center for Tropical Botany, Department of Biological Sciences, Florida International University, Miami, Florida, United States of America. TI - Large-scale patterns of turnover and Basal area change in Andean forests. SO - PLoS ONE [Electronic Resource]. 10(5):e0126594, 2015. AS - PLoS ONE. 10(5):e0126594, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - General patterns of forest dynamics and productivity in the Andes Mountains are poorly characterized. Here we present the first large-scale study of Andean forest dynamics using a set of 63 permanent forest plots assembled over the past two decades. In the North-Central Andes tree turnover (mortality and recruitment) and tree growth declined with increasing elevation and decreasing temperature. In addition, basal area increased in Lower Montane Moist Forests but did not change in Higher Montane Humid Forests. However, at higher elevations the lack of net basal area change and excess of mortality over recruitment suggests negative environmental impacts. In North-Western Argentina, forest dynamics appear to be influenced by land use history in addition to environmental variation. Taken together, our results indicate that combinations of abiotic and biotic factors that vary across elevation gradients are important determinants of tree turnover and productivity in the Andes. More extensive and longer-term monitoring and analyses of forest dynamics in permanent plots will be necessary to understand how demographic processes and woody biomass are responding to changing environmental conditions along elevation gradients through this century. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0126594 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. LG - English <83. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25966198 AU - Wang CD AU - Wang DK AU - Cao PC AU - Wang ZW AU - Wang Z AU - Wang YT FA - Wang, C D FA - Wang, D K FA - Cao, P C FA - Wang, Z W FA - Wang, Z FA - Wang, Y T IN - Wang,C D. Centre Laboratory, Weifang People's Hospital, Shandong, China. Wang,D K. Department of Neurosurgery, Weifang People's Hospital, Shandong, China. Cao,P C. Department of Neurosurgery, Weifang People's Hospital, Shandong, China. Wang,Z W. Department of Neurosurgery, Weifang People's Hospital, Shandong, China. Wang,Z. Department of Neurosurgery, Weifang People's Hospital, Shandong, China. Wang,Y T. Department of Neurosurgery, Weifang People's Hospital, Shandong, China yutingwang929@163.com. TI - Study of the relationship between the expression of nerve growth factor and aneurysm formation and prognosis. SO - Genetics & Molecular Research. 14(2):4269-75, 2015. AS - Genet Mol Res. 14(2):4269-75, 2015. NJ - Genetics and molecular research : GMR AB - We sought to investigate the effect of nerve growth factor (NGF) expression on the formation and prognosis of cerebral aneurysms. Forty-eight cases were selected following a diagnosis of cerebral aneurysm using computed tomography angiography and surgical confirmation. Thirty-four cases of healthy deaths were also chosen. The tissue was tested for NGF expression changes by reverse-transcription PCR, Western blot and histopathology, and NGF expression was compared between the cerebral aneurysm and healthy groups. The expression level of NGF in cerebral aneurysm tissue was significantly increased over that observed in control tissue. The abnormal expression of NGF is related to cerebral aneurysms. The elevated expression of NGF in cerebral aneurysms may be associated with a poor prognosis. IL - 1676-5680 DO - http://dx.doi.org/10.4238/2015.April.28.8 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <84. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25243762 AU - Livengood HM AU - Baker NA FA - Livengood, Heather M FA - Baker, Nancy A IN - Livengood,Heather M. Department of Occupational Therapy, School of Health and Rehabilitation Sciences, University of Pittsburgh , Pittsburgh, PA , USA. TI - The role of occupational therapy in vision rehabilitation of individuals with glaucoma. [Review] SO - Disability & Rehabilitation. 37(13):1202-8, 2015. AS - Disabil Rehabil. 37(13):1202-8, 2015. NJ - Disability and rehabilitation LM - Available online from MWHC library: 2005 - 2005, Available in print through MWHC library: 1999 - 2008 AB - PURPOSE: Specific to individuals with glaucoma: (1) provide an overview of the role of occupational therapists (OTs) as part of the vision rehabilitation team, (2) outline evaluation and intervention approaches provided by OTs, and (3) summarize the evidence to support those intervention approaches. AB - METHODS: Literature on vision rehabilitation and the typical practice patterns of OTs working with individuals with glaucoma are reviewed and the occupational therapy process is applied to evaluation and intervention approaches. The evidence which supports intervention approaches for individuals with glaucoma is presented. AB - RESULTS: The strength of the evidence to support common intervention approaches employed by OTs is weak or inconclusive; many studies lack quality methodological rigor. Moderate evidence supports patient education programs and strong evidence supports problem-solving and self-management strategies; this evidence is based on a limited number of studies. AB - CONCLUSION: The prevalence of eye diseases is increasing; knowledge of how visual impairment affects disability will inform resource allocation and development of rehabilitation programs that address the unique needs of individuals with glaucoma. Rehabilitation specialists are key members of the healthcare team aligned to proactively recognize and develop comprehensive rehabilitation programs to maximize individuals' function, quality of life and independence in everyday living. AB - IMPLICATIONS FOR REHABILITATION: Glaucoma is one of the four major eye diseases that may result in visual impairment leading to disability. Research supports intervention approaches and vision rehabilitation techniques used by occupational therapists to optimize the health and well-being of individuals with glaucoma. Rehabilitation specialists are key members of the healthcare team who need to be alert to subtle behaviors that may be indicative of visual impairment versus attributed to other client factors. IL - 0963-8288 DO - http://dx.doi.org/10.3109/09638288.2014.961651 PT - Journal Article PT - Review LG - English <85. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25759027 AU - Zhou M AU - Zhang Y AU - Chen X AU - Zhu J AU - Du M AU - Zhou L AU - Zhang L AU - Wang W AU - Sun G FA - Zhou, Min FA - Zhang, Yadi FA - Chen, Xulin FA - Zhu, Jianjun FA - Du, Min FA - Zhou, Liang FA - Zhang, Ling FA - Wang, Wei FA - Sun, Gengyun IN - Zhou,Min. Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, JiXi Road 218, Hefei, 230022, Anhui, People's Republic of China. TI - PTEN-Foxo1 signaling triggers HMGB1-mediated innate immune responses in acute lung injury. SO - Immunologic Research. 62(1):95-105, 2015 May. AS - Immunol Res. 62(1):95-105, 2015 May. NJ - Immunologic research AB - PTEN is a multifunctional phosphatase that regulates immune responses through a PI3K/Akt signaling cascade. HMGB1 plays an important role in the initiation of innate immune responses to induce acute lung injury (ALI). This study was designed to investigate the role of PTEN/Foxo1 signaling in the regulation of in vivo and in vitro innate immune responses in ALI. Using a mouse model of ALI, wild-type (WT) and myeloid-specific PTEN knockout (PTEN(M-KO)) mice were instilled with a recombinant HMGB1 (rHMGB1) or PBS. In some experiments, Foxo1 siRNA or non-specific siRNA was injected into mice 6 h prior to rHMGB1 instillation into lung. We found that rHMGB1 treatment in WT mice increased the expression of PTEN, Foxo1, TLR4, and NF-kappaB in alveolar macrophages from WT mice. However, macrophage-specific PTEN ablation resulted in reduced Foxo1 and TLR4 while increasing beta-catenin (Ser552) and Akt (Ser473) phosphorylation in these cells. Knockdown of Foxo1 with siRNA administration in WT mice ameliorated lung injury and inhibited myeloperoxidase activity followed by rHMGB1 treatment, which was accompanied by decreased mRNA expression coding for TNF-alpha, IL-1beta, MIP2, and IP-10. Moreover, Foxo1 knockdown inhibited the expression of TLR4-dependent IRF3 and IFN-beta both in vitro and in vivo. These results demonstrate that PTEN/Foxo1 signaling is critical for triggering HMGB1-mediated innate TLR4 activation during ALI. By identifying the molecular signaling pathways within innate immune system, our studies provide the potential therapeutic targets for ALI. IL - 0257-277X DO - http://dx.doi.org/10.1007/s12026-015-8639-z PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <86. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25752455 AU - Jung da H AU - Kim KH AU - Byeon HE AU - Park HJ AU - Park B AU - Rhee DK AU - Um SH AU - Pyo S FA - Jung, Da Hye FA - Kim, Kyung-Ho FA - Byeon, Hye Eun FA - Park, Hye Jin FA - Park, Bongkyun FA - Rhee, Dong-Kwon FA - Um, Sung Hee FA - Pyo, Suhkneung IN - Jung,Da Hye. School of Pharmacy, Sungkyunkwan University, Suwon, Gyeong gi-do, 440-746, Korea. TI - Involvement of ATF3 in the negative regulation of iNOS expression and NO production in activated macrophages. SO - Immunologic Research. 62(1):35-45, 2015 May. AS - Immunol Res. 62(1):35-45, 2015 May. NJ - Immunologic research AB - Macrophage-associated nitric oxide (NO) production plays a crucial role in the pathogenesis of tissue damage. However, negative factors that regulate NO production remains poorly understood despite its significance of NO homeostasis. Here, we show that activating transcription factor 3 (ATF3), a transcriptional regulator of cellular stress responses, was strongly induced in activated macrophages and its depletion resulted in pronounced enhancement of inducible nitric oxide synthase (iNOS) gene expression and subsequently the induction of high levels of NO production. In response to lipopolysaccharide (LPS) and IFN-gamma, ATF3 inhibited transcriptional activity of NF-kappaB by interacting with the N-terminal (1-200 amino acids) of p65 and was bound to the NF-kappaB promoter, leading to suppression of iNOS gene expression. In addition, inhibitory effects of ATF3 on iNOS and NO secretion were suppressed by inhibitor of casein kinase II (CK2) activity or its knockdown. Moreover, the levels of ATF3 were highly elevated in established cecal ligation and puncture or LPS-injected mice, a model of endotoxemia. ATF3 is also elevated in peritoneal macrophages. Collectively, our findings suggest that ATF3 regulates NO homeostasis by associating with NF-kappaB component, leading to the repression of its transcriptional activity upon inflammatory signals and points to its potential relevance for the control of cell injuries mediated by NO during macrophage activation. IL - 0257-277X DO - http://dx.doi.org/10.1007/s12026-015-8633-5 PT - Journal Article LG - English <87. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25950488 AU - Kumar S AU - Ingle H AU - Mishra S AU - Mahla RS AU - Kumar A AU - Kawai T AU - Akira S AU - Takaoka A AU - Raut AA AU - Kumar H FA - Kumar, S FA - Ingle, H FA - Mishra, S FA - Mahla, R S FA - Kumar, A FA - Kawai, T FA - Akira, S FA - Takaoka, A FA - Raut, A A FA - Kumar, H IN - Kumar,S. Laboratory of Immunology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India. Ingle,H. Laboratory of Immunology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India. Mishra,S. Laboratory of Immunology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India. Mahla,R S. Laboratory of Immunology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India. Kumar,A. Laboratory of Immunology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India. Kawai,T. Laboratory of Molecular Immunobiology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan. Akira,S. Laboratory of Host Defense, WPI Immunology Frontier Research Centre, Osaka University, Osaka, Japan. Takaoka,A. Division of Signaling in Cancer and Immunology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan. Raut,A A. Pathogenomics Laboratory, ICAR-National Institute of High Security Animal Diseases, OIE Reference lab for Avian Influenza, Bhopal, India. Kumar,H. 1] Laboratory of Immunology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India [2] Laboratory of Host Defense, WPI Immunology Frontier Research Centre, Osaka University, Osaka, Japan. TI - IPS-1 differentially induces TRAIL, BCL2, BIRC3 and PRKCE in type I interferons-dependent and -independent anticancer activity. SO - Cell Death & Disease. 6:e1758, 2015. AS - Cell Death Dis. 6:e1758, 2015. NJ - Cell death & disease AB - RIG-I-like receptors are the key cytosolic sensors for RNA viruses and induce the production of type I interferons (IFN) and pro-inflammatory cytokines through a sole adaptor IFN-beta promoter stimulator-1 (IPS-1) (also known as Cardif, MAVS and VISA) in antiviral innate immunity. These sensors also have a pivotal role in anticancer activity through induction of apoptosis. However, the mechanism for their anticancer activity is poorly understood. Here, we show that anticancer vaccine adjuvant, PolyIC (primarily sensed by MDA5) and the oncolytic virus, Newcastle disease virus (NDV) (sensed by RIG-I), induce anticancer activity. The ectopic expression of IPS-1 into type I IFN-responsive and non-responsive cancer cells induces anticancer activity. PolyIC transfection and NDV infection upregulate pro-apoptotic gene TRAIL and downregulate the anti-apoptotic genes BCL2, BIRC3 and PRKCE. Furthermore, stable knockdown of IPS-1, IRF3 or IRF7 in IFN-non-responsive cancer cells show reduced anticancer activity by suppressing apoptosis via TRAIL and anti-apoptotic genes. Collectively, our study shows that IPS-1 induces anticancer activity through upregulation of pro-apoptotic gene TRAIL and downregulation of the anti-apoptotic genes BCL2, BIRC3 and PRKCE via IRF3 and IRF7 in type I IFN-dependent and -independent manners. DO - http://dx.doi.org/10.1038/cddis.2015.122 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <88. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25630719 AU - Ren F AU - Zhou L AU - Zhang X AU - Wen T AU - Shi H AU - Xie B AU - Li Z AU - Chen D AU - Wang Z AU - Duan Z FA - Ren, Feng FA - Zhou, Li FA - Zhang, Xiangying FA - Wen, Tao FA - Shi, Hongbo FA - Xie, Bangxiang FA - Li, Zhuo FA - Chen, Dexi FA - Wang, Zheling FA - Duan, Zhongping IN - Ren,Feng. Beijing Artificial Liver Treatment & Training Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China. TI - Endoplasmic reticulum stress-activated glycogen synthase kinase 3beta aggravates liver inflammation and hepatotoxicity in mice with acute liver failure. SO - Inflammation. 38(3):1151-65, 2015. AS - Inflammation. 38(3):1151-65, 2015. NJ - Inflammation AB - Endoplasmic reticulum stress (ER stress) has been increasingly recognized as an important mechanism in various liver diseases. However, its intrinsic physiological role in acute liver failure (ALF) remains largely undetermined. This study aimed to examine how ER stress orchestrates glycogen synthase kinase 3beta (GSK3beta) and inflammation to affect ALF. In a murine ALF model induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS), 4-phenylbutyric acid (4-PBA) is to be administered to relieve ER stress. The lethality rate, liver damage, cytokine expression, and the activity of GSK3beta were evaluated. How to regulate LPS-induced inflammation and TNF-alpha-induced hepatocyte apoptosis by ER stress was investigated in vitro. In vivo, ER stress was triggered in the liver with the progression of mice ALF model. ER stress was essential for the development of ALF because ER stress inhibition by 4-PBA ameliorated the liver damage through decreasing liver inflammation and hepatocyte apoptosis. 4-PBA also decreased GSK3beta activity in the livers of ALF mice. In vitro, ER stress induced by tunicamycin synergistically increased LPS-triggered pro-inflammatory cytokine induction and promoted the activation of nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathway in bone marrow-derived macrophages; moreover, tunicamycin also cooperated with TNF-alpha to increase hepatocyte apoptosis. ER stress promoted LPS-triggered inflammation depending on GSK3beta activation because inhibition of GSK3beta by SB216763, the specific inhibitor of GSK3beta, resulted in downregulation of pro-inflammatory genes. ER stress contributes to liver inflammation and hepatotoxicity in ALF, particularly by regulating GSK3beta, and is therefore a potential therapeutic target for ALF. IL - 0360-3997 DO - http://dx.doi.org/10.1007/s10753-014-0080-2 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <89. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25879970 AU - Chen H AU - Gao J AU - Su S AU - Zhang X AU - Wang Z FA - Chen, Hong FA - Gao, Jiyang FA - Su, Shaojie FA - Zhang, Xu FA - Wang, Zhihua TI - A visual-aided wireless monitoring system design for total hip replacement surgery. SO - IEEE Transactions on Biomedical Circuits & Systems. 9(2):227-36, 2015 Apr. AS - IEEE trans. biomed. circuits syst.. 9(2):227-36, 2015 Apr. NJ - IEEE transactions on biomedical circuits and systems AB - To improve the positioning accuracy of implants in Total Hip Replacement (THR) surgeries, a visual-aided wireless monitoring system for THR surgery is proposed in this paper. This system aims to measure and display the contact distribution and relative pose between femoral head and acetabulum prosthesis during the surgery to help surgeons obtain accurate position of implants. The system consists of two parts: the Sensors Array Measuring System (SAMS) and the display part. The SAMS is composed of a sensors array (including contact sensors and an image sensor), signal conditioning circuits, a low power microcontroller (MCU), and a low-power transceiver. The SAMS is designed to estimate the relative pose of femoral head component to acetabular component. The display part processes the data from sensors and demonstrates the contact distribution and the pose of the prothesis during the surgery in 3-D graphics. The two parts of the system communicate with each other on an RF link at the band of 400 MHz. The signal conditioning circuits have been designed and fabricated in 0.18 mum CMOS process. Testing results show that the resolution of the signal conditioning circuits is 60.1 mu Vpp (1.35 g) with +/-100 mVpp input. The chip can operate under 1.2-to-3.6 V supply voltage for single battery applications with 116-160 mu A current consumption. The system has been verified by the simulation with rotation quaternion and translation vector. The experimental results show that the contact distribution and relative pose of the two components could be measured and demonstrated in real time. The relative error of rotation is less than 8% and the actual relative error of translation is less than 10%. IL - 1932-4545 DO - http://dx.doi.org/10.1109/TBCAS.2015.2416253 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <90. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25921661 AU - Pittman ME AU - Brunt EM FA - Pittman, Meredith E FA - Brunt, Elizabeth M IN - Pittman,Meredith E. Department of Pathology, Johns Hopkins Medical Institutions, 401 North Broadway, Baltimore, MD 21231, USA. Brunt,Elizabeth M. Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA. Electronic address: EBrunt@path.wustl.edu. TI - Anatomic pathology of hepatocellular carcinoma: histopathology using classic and new diagnostic tools. [Review] SO - Clinics in Liver Disease. 19(2):239-59, 2015 May. AS - Clin Liver Dis. 19(2):239-59, 2015 May. NJ - Clinics in liver disease LM - Available online from MWHC library: May 1997 - present AB - Hepatocellular carcinoma can be diagnosed on a needle biopsy of the liver; however, uncertainty may arise because of the inherent complexity of liver histology. This article aims to provide practicing pathologists with tools for the approach to mass-directed liver biopsies clinically concerning for hepatocellular carcinoma. The examination of routine hematoxylin-eosin stains and the use of ancillary histochemical and immunohistochemical stains are discussed. Sections reviewing liver carcinoma with biphenotypic differentiation and the challenge of dysplastic nodules are included. Copyright © 2015 Elsevier Inc. All rights reserved. IL - 1089-3261 DO - http://dx.doi.org/10.1016/j.cld.2015.01.003 PT - Journal Article PT - Review LG - English <91. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25837878 AU - Ke L AU - Yan G AU - Wang Y AU - Wang Z AU - Liu D FA - Ke, Lei FA - Yan, Guozheng FA - Wang, Yongbing FA - Wang, Zhiwu FA - Liu, Dasheng IN - Ke,Lei. 1 Laboratory of Medical Precision Engineering and Intelligent Systems, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiaotong University, Shanghai - China. TI - Design and evaluation of an intelligent artificial anal sphincter system powered by an adaptive transcutaneous energy transfer system. SO - International Journal of Artificial Organs. 38(3):154-60, 2015 Mar. AS - Int J Artif Organs. 38(3):154-60, 2015 Mar. NJ - The International journal of artificial organs AB - OBJECTIVES: The aim of this study was to optimize an intelligent artificial anal sphincter system (AASS) II for patients with severe fecal incontinence. AB - METHODS: Redesigning and integrating a pressure sensor into the sphincter prosthesis allows us to reduce the sensor volume and makes it suitable for a chronic, ambulatory application. Furthermore, a close-loop frequency control method was designed for the transcutaneous energy transfer system. Finally, a longer working time of the implanted device was obtained by the low-power design of the hardware and software. The new model was implanted in 2 dogs and studied for periods of up to 5 weeks. AB - RESULTS: The output voltage induced on the load of 30 OMEGA, for a variation range in k of 0.12 ~ 0.42, was maintained at approximately 6.8 V with a frequency control range of the 270 ~ 320 kHz. The minimum and maximum output voltages of the pressure sensor were found to be 1.7 V and 2.34 V, respectively, which corresponded to a pressure range of 90 ~ 120 kPa with maximum change rate of approximately 3.7% caused by the temperature variations. Moreover, compared with AASS I, the low-power design resulting in 94% reduction in power consumption. AB - CONCLUSIONS: The efficacy of the device in achieving continence and sensing the need to defecate was assessed in an animal model. The technical concept and the design of the AASS II turned out to be capable of fulfilling the medical requirements. IL - 0391-3988 DO - http://dx.doi.org/10.5301/ijao.5000386 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <92. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 24668234 AU - Arcos-Machancoses JV AU - Marin-Reina P AU - Romaguera-Salort E AU - Garcia-Camunas Y AU - Perez-Aytes A AU - Vento M FA - Arcos-Machancoses, Jose Vicente FA - Marin-Reina, Purificacion FA - Romaguera-Salort, Eugenia FA - Garcia-Camunas, Yolanda FA - Perez-Aytes, Antonio FA - Vento, Maximo IN - Arcos-Machancoses,Jose Vicente. Department of Pediatrics, University & Polytechnic Hospital La Fe, Valencia, Spain. TI - Postnatal development of fetuses with a single umbilical artery: differences between malformed and non-malformed infants. SO - World Journal of Pediatrics. 11(1):61-6, 2015 Feb. AS - World J Pediatr. 11(1):61-6, 2015 Feb. NJ - World journal of pediatrics : WJP AB - BACKGROUND: The presence of a single umbilical artery (SUA) is a fetal soft marker of congenital abnormalities. Among the most common related malformations, there are cardiological, nephrourological and digestive anomalies, most of which are considered to have a vascular etiology. There is an association between increased incidence of intrauterine growth retardation and adverse perinatal indicators, but whether this association is due to related anomalies or isolated SUA (iSUA) is controvisal. AB - METHODS: We reviewed 96 cases of iSUA and non-isolated SUA (niSUA), diagnosed in a period of two years in a referral hospital for high-risk pregnancies. Data on prenatal explorations, including fetal ultrasonography and karyotyping, were obtained. niSUA was diagnosed when no malformations were found prenatally or in postnatal evaluation. AB - RESULTS: Sixty-six newborns (68.8%) had no other anomalies and 30 (31.3%) presented with a variety of malformations including heart diseases, urophaties, digestive, nervous and musculoskeletal disorders, genetic abnormalities and complex malformations. Cardiological and nephrourological abnormalities were found to be the most frequent association with a SUA (both in 23.8% of malformed SUA newborns). Intrauterine growth restriction was not higher in iSUA newborns than in a normal population. Ultrasound allowed optimal prenatal diagnosis in most cases. AB - CONCLUSIONS: The prognosis of the fetus with a SUA is determined by the presence of other malformations observed by an expert sonographer. If no other findings are made, only a routine physical examination should be performed in newborns, but no other complementary examinations are required. DO - http://dx.doi.org/10.1007/s12519-014-0471-3 PT - Journal Article LG - English <93. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25815839 AU - Yang SH AU - Lin JC AU - Wu SY AU - Huang KL AU - Jung F AU - Ma MC AU - Wang Hsu GS AU - Jow GM FA - Yang, Shih-Hsing FA - Lin, Jau-Chen FA - Wu, Shu-Yu FA - Huang, Kun-Lun FA - Jung, Fang FA - Ma, Ming-Chieh FA - Wang Hsu, Guoo-Shyng FA - Jow, Guey-Mei IN - Yang,Shih-Hsing. Department of Respiratory Therapy, Fu Jen Catholic University, New Taipei City, Taiwan; P.h.D Program in Nutrition and Food Science, Fu Jen Catholic University, New Taipei City, Taiwan. Lin,Jau-Chen. Department of Respiratory Therapy, Fu Jen Catholic University, New Taipei City, Taiwan. Wu,Shu-Yu. Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei, Taiwan. Huang,Kun-Lun. Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei, Taiwan. Jung,Fang. Department of Respiratory Therapy, Fu Jen Catholic University, New Taipei City, Taiwan. Ma,Ming-Chieh. School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan. Wang Hsu,Guoo-Shyng. P.h.D Program in Nutrition and Food Science, Fu Jen Catholic University, New Taipei City, Taiwan; Department of Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan. Jow,Guey-Mei. School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan. TI - Membrane translocation of IL-33 receptor in ventilator induced lung injury. SO - PLoS ONE [Electronic Resource]. 10(3):e0121391, 2015. AS - PLoS ONE. 10(3):e0121391, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - Ventilator-induced lung injury is associated with inflammatory mechanism and causes high mortality. The objective of this study was to discover the role of IL-33 and its ST2 receptor in acute lung injury induced by mechanical ventilator (ventilator-induced lung injury; VILI). Male Wistar rats were intubated after tracheostomy and received ventilation at 10 cm H2O of inspiratory pressure (PC10) by a G5 ventilator for 4 hours. The hemodynamic and respiratory parameters were collected and analyzed. The morphological changes of lung injury were also assessed by histological H&E stain. The dynamic changes of lung injury markers such as TNF-alpha and IL-1beta were measured in serum, bronchoalveolar lavage fluid (BALF), and lung tissue homogenization by ELISA assay. During VILI, the IL-33 profile change was detected in BALF, peripheral serum, and lung tissue by ELISA analysis. The Il-33 and ST2 expression were analyzed by immunohistochemistry staining and western blot analysis. The consequence of VILI by H&E stain showed inducing lung congestion and increasing the expression of pro-inflammatory cytokines such as TNF-alpha and IL-1beta in the lung tissue homogenization, serum, and BALF, respectively. In addition, rats with VILI also exhibited high expression of IL-33 in lung tissues. Interestingly, the data showed that ST2L (membrane form) was highly accumulated in the membrane fraction of lung tissue in the PC10 group, but the ST2L in cytosol was dramatically decreased in the PC10 group. Conversely, the sST2 (soluble form) was slightly decreased both in the membrane and cytosol fractions in the PC10 group compared to the control group. In conclusion, these results demonstrated that ST2L translocation from the cytosol to the cell membranes of lung tissue and the down-expression of sST2 in both fractions can function as new biomarkers of VILI. Moreover, IL-33/ST2 signaling activated by mechanically responsive lung injury may potentially serve as a new therapy target. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0121391 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <94. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25815507 AU - Koo M AU - Hwang JH FA - Koo, Malcolm FA - Hwang, Juen-Haur IN - Koo,Malcolm. Department of Medical Research, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin, Chiayi, Taiwan; Dalla Lana School of Public Health, University of Toronto, Ontario, Canada. Hwang,Juen-Haur. School of Medicine, Tzu Chi University, Hualien, Taiwan; Department of Otolaryngology, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin, Chiayi, Taiwan; Sleep Center, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin, Chiayi, Taiwan. TI - Risk of sudden sensorineural hearing loss in patients with common preexisting sensorineural hearing impairment: a population-based study in Taiwan. SO - PLoS ONE [Electronic Resource]. 10(3):e0121190, 2015. AS - PLoS ONE. 10(3):e0121190, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - OBJECTIVE: The role of preexisting sensorineural hearing impairment on the risk for sudden sensorineural hearing loss (SSHL) is still unclear. In this study, we aimed to assess the risk of SSHL in patients with common preexisting sensorineural hearing impairment using population-based data. AB - METHODS: A population-based case-control study design was used to analyze claims data between January 2001 and December 2011 obtained from the Taiwan National Health Insurance Research Database. The cases consisted of 514 patients with SSHL and the controls were frequency matched to 2,570 cases by sex, 10-year age group, and year of index date. Common sensorineural hearing impairments were retrospectively assessed in the cases and controls. Associations between sensorineural hearing impairment and risk of SSHL were evaluated using unconditional univariate and multivariate logistic regression analyses. AB - RESULTS: The mean age for the 3,084 study subjects was 53.1 years (standard deviation, S.D. = 15.6). Of the 514 cases, 49 (9.5%) had sensorineural hearing impairment while only 44 (1.7%) of the 2,570 controls had the same condition. Univariate logistic regression analyses indicated that preexisting sensorineural hearing impairment was significantly associated with SSHL (odds ratio, OR = 6.05, p < 0.001). Other comorbidities including hypertension, diabetes mellitus, and hyperlipidemia also showed significant associations with SSHL. Similar results were obtained when the association between SSHL and sensorineural hearing impairment was adjusted with either all the covariates (adjusted OR = 6.22, p < 0.001) or with only those selected using a backward elimination procedure (adjusted OR = 6.20, p < 0.001). AB - CONCLUSIONS: Results from this population-based case-control study revealed that common sensorineural hearing impairment might be a novel risk factor for SSHL. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0121190 PT - Journal Article LG - English <95. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25811133 AU - Bumpass DB AU - Buchowski JM AU - Park A AU - Gray BL AU - Agarwal R AU - Baty J AU - Zebala LP AU - Riew KD AU - Santiago P AU - Ray WZ AU - Wright NM FA - Bumpass, David B FA - Buchowski, Jacob M FA - Park, Andrew FA - Gray, Benjamin L FA - Agarwal, Rashmi FA - Baty, Jack FA - Zebala, Lukas P FA - Riew, K Daniel FA - Santiago, Paul FA - Ray, Wilson Z FA - Wright, Neill M IN - Bumpass,David B. *Department of Orthopaedic Surgery, Washington University, St. Louis, MO +Division of Biostatistics, Washington University School of Medicine, St. Louis, MO; and ++Department of Neurosurgery, Washington University, St. Louis, MO. TI - An update on civilian spinal gunshot wounds: treatment, neurological recovery, and complications. SO - Spine. 40(7):450-61, 2015 Apr 1. AS - Spine. 40(7):450-61, 2015 Apr 1. NJ - Spine LM - Available online from MWHC library: 1996 - present, Available in print through MWHC library: 1999 - February 2003 AB - STUDY DESIGN: Retrospective analysis of inpatient and outpatient data from a single academic trauma center. AB - OBJECTIVE: To test the effectiveness of a conservative treatment algorithm for civilian spinal gunshot wounds (CSGSWs) by comprehensively evaluating neurological status and recovery, fracture type, concomitant injuries, indications for surgery, and complications. AB - SUMMARY OF BACKGROUND DATA: Few large studies exist to guide treatment of CSGSWs, and none have been published in nearly 20 years. AB - METHODS: A search of International Classification of Diseases, Ninth Revision (ICD-9) codes was performed for all hospital patients treated from 2003 to 2011 by either neurosurgery or orthopedic surgery to identify 159 consecutive patients who sustained CSGSWs. Mean follow-up was 13.6 months. American Spinal Injury Association grading was used to assess neurological injury. AB - RESULTS: Fifty percent of patients had neurological deficits from CSGSW. Complete spinal injury was the most common injury grade; thoracic injuries had the most risk of complete injury (P < 0.001). Nearly 80% of patients had concomitant injuries to other organs. Operative treatment was more likely in patients with severe neurological injuries (P = 0.008) but was not associated with improved neurological outcomes (P = 1.00). Nonoperative treatment did not lead to any cases of late spinal instability or neurological deterioration. Overall, 31% of patients had an improvement of at least 1 American Spinal Injury Association grade by final follow-up. Nearly half of patients experienced at least 1 GSW-related complication; risk of complications was associated with neurological injury grade (P < 0.001) and operative treatment (P = 0.04). AB - CONCLUSION: The vast majority of CSGSWs should be managed nonoperatively, regardless of neurological grade or number of spinal columns injured. Indications for surgery include spinal infection and persistent cerebrospinal fluid leaks. AB - LEVEL OF EVIDENCE: 3. IL - 0362-2436 DO - http://dx.doi.org/10.1097/BRS.0000000000000797 PT - Journal Article LG - English <96. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25608245 AU - Umeda A AU - Saeki N AU - Matsumoto C AU - Nakao M AU - Kawamoto M FA - Umeda, Ayako FA - Saeki, Noboru FA - Matsumoto, Chikako FA - Nakao, Masakazu FA - Kawamoto, Masashi IN - Umeda,Ayako. *Department of Anesthesiology, JA Hiroshima General Hospital, Hiroshima, Japan; and +Department of Anesthesiology and Critical Care, Hiroshima University, Hiroshima, Japan. TI - Abdominal aortic injury during vertebroplasty. SO - Spine. 40(7):E439-41, 2015 Apr 1. AS - Spine. 40(7):E439-41, 2015 Apr 1. NJ - Spine LM - Available online from MWHC library: 1996 - present, Available in print through MWHC library: 1999 - February 2003 AB - STUDY DESIGN: Case report. AB - OBJECTIVE: To describe an intraoperative complication occurring from abdominal aortic penetration during a vertebroplasty procedure for vertebral fractures on Th12 and L1. AB - SUMMARY OF BACKGROUND DATA: A vertebroplasty is a minimally invasive and widely performed procedure in elderly and high-risk patients, although there is a risk of life-threatening complications including aortic injury. However, little is known about the treatment of iatrogenic aortic penetration occurring during a vertebroplasty. AB - METHODS: An 80-year-old female underwent a scheduled vertebroplasty procedure. When the needle was advanced into the L1 vertebral body, arterial blood spurted out from the needle hub and fluoroscopic imaging revealed penetration of the aorta. To minimize bleeding, we depressed blood pressure and kept the needle in place. While vital signs were maintained, we prepared for blood transfusion and circulation monitoring and consulted a cardiothoracic surgeon and a cardiologist. Contrast medium injected via the needle revealed that a hematoma had formed to shift the aortic wall beyond the needle. Circulation was stable while pressure of the needle decreased, thus the hematoma was thought to have become coagulated and the needle was cautiously withdrawn. AB - RESULTS: After placing the patient in a supine position, aortic angiography revealed no leakage around the aorta and she was transferred to the intensive care unit. On postoperative day 1, no leakage around the aorta was confirmed on computed tomographic scans and the patient was extubated. During the 2-year follow-up period, no arterial complication was observed. AB - CONCLUSION: Conservative treatment is optional for accidental aortic penetration during a vertebroplasty when a tamponade effect is expected. In cases with circulatory collapse, when the tamponade effect seems insufficient or a free wall rupture is suspected, prompt removal of the needle and surgical repair should be considered. AB - LEVEL OF EVIDENCE: 5. IL - 0362-2436 DO - http://dx.doi.org/10.1097/BRS.0000000000000780 PT - Case Reports PT - Journal Article LG - English <97. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25803032 AU - Garcia-Carracedo D AU - Yu CC AU - Akhavan N AU - Fine SA AU - Schonleben F AU - Maehara N AU - Karg DC AU - Xie C AU - Qiu W AU - Fine RL AU - Remotti HE AU - Su GH FA - Garcia-Carracedo, Dario FA - Yu, Chih-Chieh FA - Akhavan, Nathan FA - Fine, Stuart A FA - Schonleben, Frank FA - Maehara, Naoki FA - Karg, Dillon C FA - Xie, Chuangao FA - Qiu, Wanglong FA - Fine, Robert L FA - Remotti, Helen E FA - Su, Gloria H IN - Garcia-Carracedo,Dario. The Department of Pathology, Columbia University Medical Center, New York, New York, United States of America; Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America. Yu,Chih-Chieh. The Department of Pathology, Columbia University Medical Center, New York, New York, United States of America; Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America. Akhavan,Nathan. Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America. Fine,Stuart A. Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America. Schonleben,Frank. The Department of Vascular Surgery in the Hospital of the University of Munich, Grosshadern, Germany. Maehara,Naoki. Department of Surgical Oncology and Regulation of Organ Function, Miyazaki University School of Medicine, Miyazaki, Japan. Karg,Dillon C. Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America. Xie,Chuangao. The Department of Pathology, Columbia University Medical Center, New York, New York, United States of America; Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America. Qiu,Wanglong. The Department of Pathology, Columbia University Medical Center, New York, New York, United States of America; Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America. Fine,Robert L. Department of Medicine, Columbia University Medical Center, New York, New York, United States of America; Pancreas Center, Columbia University Medical Center, New York, New York, United States of America. Remotti,Helen E. The Department of Pathology, Columbia University Medical Center, New York, New York, United States of America; Department of Surgical Oncology and Regulation of Organ Function, Miyazaki University School of Medicine, Miyazaki, Japan. Su,Gloria H. The Department of Pathology, Columbia University Medical Center, New York, New York, United States of America; Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, New York, United States of America; Pancreas Center, Columbia University Medical Center, New York, New York, United States of America; Department of Otolaryngology and Head and Neck Surgery, Columbia University Medical Center, New York, New York, United States of America. TI - Smad4 loss synergizes with TGFalpha overexpression in promoting pancreatic metaplasia, PanIN development, and fibrosis. SO - PLoS ONE [Electronic Resource]. 10(3):e0120851, 2015. AS - PLoS ONE. 10(3):e0120851, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - AIMS: While overexpression of TGFalpha has been reported in human pancreatic ductal adenocarcinoma (PDAC), mice with overexpressed TGFalpha develop premalignant pancreatic acinar-to-ductal metaplasia (ADM) but not PDAC. TGF-beta signaling pathway is pivotal to the development of PDAC and tissue fibrosis. Here we sought to investigate the interplay between TGFalpha and TGF-beta signaling in pancreatic tumorigenesis and fibrosis, namely via Smad4 inactivation. AB - METHODS: The MT-TGFalpha mouse was crossed with a new Smad4 conditional knock-out mouse (Smad4flox/flox;p48-Cre or S4) to generate Smad4flox/flox;MT-TGFalpha;p48-Cre (STP). After TGFalpha overexpression was induced with zinc sulfate water for eight months, the pancreata of the STP, MT-TGFalpha, and S4 mice were examined for tumor development and fibrotic responses. PanIN lesions and number of ducts were counted, and proliferation was measured by Ki67 immunohistochemistry (IHC). Qualitative analysis of fibrosis was analyzed by Trichrome Masson and Sirius Red staining, while vimentin was used for quantification. Expression analyses of fibrosis, pancreatitis, or desmoplasia associated markers (alpha-SMA, Shh, COX-2, Muc6, Col1a1, and Ctgf) were performed by IHC and/or qRT-PCR. AB - RESULTS: Our STP mice exhibited advanced ADM, increased fibrosis, increased numbers of PanIN lesions, overexpression of chronic pancreatitis-related marker Muc6, and elevated expression of desmoplasia-associated marker Col1A1, compared to the MT-TGFalpha mice. The inactivation of Smad4 in the exocrine compartment was responsible for both the enhanced PanIN formation and fibrosis in the pancreas. The phenotype of the STP mice represents a transient state from ADMs to PanINs, closely mimicking the interface area seen in human chronic pancreatitis associated with PDAC. AB - CONCLUSION: We have documented a novel mouse model, the STP mice, which displayed histologic presentations reminiscent to those of human chronic pancreatitis with signs of early tumorigenesis. The STP mice could be a suitable animal model for interrogating the transition of chronic pancreatitis to pancreatic cancer. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0120851 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English <98. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25799566 AU - Yang S AU - Lin S AU - Fu Q AU - Cai B AU - Kong F AU - Huang G AU - Li F AU - Wang H FA - Yang, Shiping FA - Lin, Shaomin FA - Fu, Qiang FA - Cai, Baizhen FA - Kong, Fei FA - Huang, Guang FA - Li, Fafen FA - Wang, Han IN - Yang,Shiping. Department of Radiation Oncology, Hainan Province People's Hospital, Haikou, Hainan, PR China. Lin,Shaomin. Department of Radiation Oncology, Hainan Province People's Hospital, Haikou, Hainan, PR China. Fu,Qiang. Department of Radiation Oncology, Hainan Province People's Hospital, Haikou, Hainan, PR China. Cai,Baizhen. Department of Radiation Oncology, Hainan Province People's Hospital, Haikou, Hainan, PR China. Kong,Fei. Department of Radiation Oncology, Hainan Province People's Hospital, Haikou, Hainan, PR China. Huang,Guang. Department of Radiation Oncology, Hainan Province People's Hospital, Haikou, Hainan, PR China. Li,Fafen. Department of Radiation Oncology, Hainan Province People's Hospital, Haikou, Hainan, PR China. Wang,Han. Department of Physiology, Hainan Medical college, Haikou, Hainan, PR China. TI - The effect of adjuvant chemotherapy on survival in patients with residual nasopharyngeal carcinoma after undergoing concurrent chemoradiotherapy. SO - PLoS ONE [Electronic Resource]. 10(3):e0120019, 2015. AS - PLoS ONE. 10(3):e0120019, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - BACKGROUND: Guidelines from the U.S. National Comprehensive Cancer Network have recommended use of concurrent chemoradiotherapy (CCRT), followed by a 3-cycles combination of platinum and 5-fluorouracil chemotherapy as standard treatment for nasopharyngeal carcinoma (NPC). The benefits of CCRT for treatment of locally advanced NPC have been established. Whether platinum and 5-fluorouracil chemotherapy should be routinely added to locally advanced NPC after CCRT is still open to debate. Whether adjuvant chemotherapy provides an additional survival benefit for the subgroup of patients with residual nasopharyngeal carcinoma who have undergone CCRT is also unclear. This retrospective study was initiated to determine the survival benefit of adjuvant chemotherapy (AC) in residual NPC patients who have undergone concurrent chemoradiotherapy. AB - METHODS: The retrospective study included 155 nasopharyngeal carcinoma patients who had local residual lesions after the platinum-based CCRT without or with AC. Kaplan-Meier analysis and the log-rank test were used to estimate overall survival (OS), failure-free survival (FFS), local relapse-free survival (LRFS) and distant metastasis-free survival (DMFS). AB - RESULTS: Median follow-up was 47 months. Adjuvant cisplatin or nedaplatin plus 5-fluorouracil chemotherapy did not significantly improve 3-year OS, LRFS, FFS, and DMFS for patients with residual nasopharyngeal carcinoma after undergoing CCRT. The 3-year OS rates for the no-AC group and AC group were 71.6% and 73.7%, respectively (P= 0.44). The 3-year FFS rates for no-AC group and AC group were 57.5% and 66.9%, respectively ((P= 0.19). The 3-year LRFS rates for no-AC group and AC group were 84.7% and 87.9%, respectively ((P= 0.51). The 3-year DMFS rates for no-AC group and AC group were 71.4% and 77.4%, respectively ((P= 0.23). AB - CONCLUSIONS: Since we did not find sufficient data to support significant survival in 3-year OS, LRFS, FFS, and DMFS, whether Adjuvant cisplatin or nedaplatin and 5-fluorouracil chemotherapy should be routinely added to residual nasopharyngeal carcinoma patients after undergoing CCRT remain uncertain. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0120019 PT - Journal Article LG - English <99. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25798860 AU - Chai J AU - Feng X AU - Zhang L AU - Chen S AU - Cheng Y AU - He X AU - Yang Y AU - He Y AU - Wang H AU - Wang R AU - Chen W FA - Chai, Jin FA - Feng, Xinchan FA - Zhang, Liangjun FA - Chen, Sheng FA - Cheng, Ying FA - He, Xiaochong FA - Yang, Yingxue FA - He, Yu FA - Wang, Huaizhi FA - Wang, Rongquan FA - Chen, Wensheng IN - Chai,Jin. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. Feng,Xinchan. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. Zhang,Liangjun. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. Chen,Sheng. Department of Pediatrics, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. Cheng,Ying. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. He,Xiaochong. School of Nursing, Third Military Medical University, Chongqing, P.R. China. Yang,Yingxue. Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China. He,Yu. Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. Wang,Huaizhi. Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. Wang,Rongquan. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. Chen,Wensheng. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China. TI - Hepatic expression of detoxification enzymes is decreased in human obstructive cholestasis due to gallstone biliary obstruction. SO - PLoS ONE [Electronic Resource]. 10(3):e0120055, 2015. AS - PLoS ONE. 10(3):e0120055, 2015. NJ - PloS one LM - Available online through MWHC library: 2006 - present AB - BACKGROUND & AIMS: Levels of bile acid metabolic enzymes and membrane transporters have been reported to change in cholestasis. These alterations (e.g. CYP7A1 repression and MRP4 induction) are thought to be adaptive responses that attenuate cholestatic liver injury. However, the molecular mechanisms of these adaptive responses in human obstructive cholestasis due to gallstone biliary obstruction remain unclear. AB - METHODS: We collected liver samples from cholestatic patients with biliary obstruction due to gallstones and from control patients without liver disease (n = 22 per group). The expression levels of bile acid synthetic and detoxification enzymes, membrane transporters, and the related nuclear receptors and transcriptional factors were measured. AB - RESULTS: The levels of bile acid synthetic enzymes, CYP7B1 and CYP8B1, and the detoxification enzyme CYP2B6 were increased in cholestatic livers by 2.4-fold, 2.8-fold, and 1.9-fold, respectively (p<0.05). Conversely, the expression levels of liver detoxification enzymes, UGT2B4/7, SULT2A1, GSTA1-4, and GSTM1-4, were reduced by approximately 50% (p<0.05) in human obstructive cholestasis. The levels of membrane transporters, OSTbeta and OCT1, were increased 10.4-fold and 1.8-fold, respectively, (p<0.05), whereas those of OSTalpha, ABCG2 and ABCG8 were all decreased by approximately 40%, (p<0.05) in human cholestatic livers. Hepatic nuclear receptors, VDR, HNF4alpha, RXRalpha and RARalpha, were induced (approximately 2.0-fold, (p<0.05) whereas FXR levels were markedly reduced to 44% of control, (p<0.05) in human obstructive cholestasis. There was a significantly positive correlation between the reduction in FXR mRNA and UGT2B4/7, SULT2A1, GSTA1, ABCG2/8 mRNA levels in livers of obstructive cholestatic patients (p<0.05). AB - CONCLUSIONS: The levels of hepatic detoxification enzymes were significantly decreased in human obstructive cholestasis, and these decreases were positively associated with a marked reduction of FXR levels. These findings are consistent with impaired detoxification ability in human obstructive cholestasis. IL - 1932-6203 DO - http://dx.doi.org/10.1371/journal.pone.0120055 PT - Journal Article PT - Research Support, Non-U.S. Gov't LG - English <100. > VN - Ovid Technologies DB - Ovid MEDLINE(R) UI - 25784652 AU - Shu X AU - Purdue MP AU - Ye Y AU - Wood CG AU - Chen M AU - Wang Z AU - Albanes D AU - Pu X AU - Huang M AU - Stevens VL AU - Diver WR AU - Gapstur SM AU - Virtamo J AU - Chow WH AU - Tannir NM AU - Dinney CP AU - Rothman N AU - Chanock SJ AU - Wu X FA - Shu, Xiang FA - Purdue, Mark P FA - Ye, Yuanqing FA - Wood, Christopher G FA - Chen, Meng FA - Wang, Zhaoming FA - Albanes, Demetrius FA - Pu, Xia FA - Huang, Maosheng FA - Stevens, Victoria L FA - Diver, W Ryan FA - Gapstur, Susan M FA - Virtamo, Jarmo FA - Chow, Wong-Ho FA - Tannir, Nizar M FA - Dinney, Colin P FA - Rothman, Nathaniel FA - Chanock, Stephen J FA - Wu, Xifeng IN - Shu,Xiang. Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Purdue,Mark P. Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA. Ye,Yuanqing. Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Wood,Christopher G. Urology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Chen,Meng. Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Wang,Zhaoming. Cancer Genomics Research Laboratory, SAIC-Frederick Inc., National Cancer Institute-Frederick, Frederick, Maryland, USA. Albanes,Demetrius. Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA. Pu,Xia. Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Huang,Maosheng. Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Stevens,Victoria L. Epidemiology Research Program, American Cancer Society, Atlanta, Georgia, USA. Diver,W Ryan. Epidemiology Research Program, American Cancer Society, Atlanta, Georgia, USA. Gapstur,Susan M. Epidemiology Research Program, American Cancer Society, Atlanta, Georgia, USA. Virtamo,Jarmo. Department of Chronic Disease Prevention, National Institute for Health and Welfare, Helsinki, Finland. Chow,Wong-Ho. Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Tannir,Nizar M. Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Dinney,Colin P. Urology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. Rothman,Nathaniel. Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA. Chanock,Stephen J. Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA. Wu,Xifeng. Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA. TI - Multilevel-analysis identify a cis-expression quantitative trait locus associated with risk of renal cell carcinoma. SO - Oncotarget. 6(6):4097-109, 2015 Feb 28. AS - Oncotarget. 6(6):4097-109, 2015 Feb 28. NJ - Oncotarget AB - We conducted multilevel analyses to identify potential susceptibility loci for renal cell carcinoma (RCC), which may be overlooked in traditional genome-wide association studies (GWAS). A gene set enrichment analysis was performed utilizing a GWAS dataset comprised of 894 RCC cases and 1,516 controls using GenGen, SNP ratio test, and ALIGATOR. The antigen processing and presentation pathway was consistently significant (P = 0.001, = 0.004, and < 0.001, respectively). Versatile gene-based association study approach was applied to the top-ranked pathway and identified the driven genes. By comparing the expression of the genes in RCC tumor and adjacent normal tissues, we observed significant overexpression of HLA genes in tumor tissues, which was also supported by public databases. We sought to validate genetic variants in antigen processing and presentation pathway in an independent GWAS dataset comprised of 1,311 RCC cases and 3,424 control subjects from the National Cancer Institute; one SNP, rs1063355, was significant in both populations (P(meta-analysis) = 9.15 x 10-4, P(heterogeneity) = 0.427). Strong correlation indicated that rs1063355 was a cis-expression quantitative trait loci which associated with HLA-DQB1 expression (Spearman's rank r = -0.59, p = 5.61 x 10-6). The correlation was further validated using a public dataset. Our results highlighted the role of immune-related pathway and genes in the etiology of RCC. IL - 1949-2553 PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't LG - English